An ultrasensitive bioluminogenic probe of γ-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis

Li, Shuang; Hu, Rui; Yang, Chenlin; Zhang, Xin; Zeng, Yi; Wang, Shuangqing; Guo, Xudong; Li, Yang; Cai, Xiaopin; Li, Shirui; Han, Chengwu; Yang, Guoqiang

doi:10.1016/j.bios.2017.06.059

Cited by 26 publications

(19 citation statements)

References 28 publications

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“…directly employed d-luciferin and designed another BL probe (24)t hrough the introductiono fg-Glu and as elf-immolativel inker,P ABA. [49] A3 70-fold increment of bioluminescence intensity was observed after incubation with GGT for 80 min, which was highly sensitivet od etect the GGT level in solution, with ad etection limit as low as 0.66 mU L À1 ( Figure 9D). Through BL of GGT activity in cells, probe 24 was amenable to differentiate ES-2-luc ovarian cancer cells from NIH-3T3-luc cells.…”

Section: Bioluminescencei Maging Probes For Ggtmentioning

confidence: 96%

“…In addition to the use of aminoluciferin as a bioluminescence reporter, Li et al. directly employed d ‐luciferin and designed another BL probe ( 24 ) through the introduction of γ‐Glu and a self‐immolative linker, PABA . A 370‐fold increment of bioluminescence intensity was observed after incubation with GGT for 80 min, which was highly sensitive to detect the GGT level in solution, with a detection limit as low as 0.66 mU L −1 (Figure D).…”

Section: Bioluminescence Imaging Probes For Ggtmentioning

confidence: 99%

“….Copyright: 2017, American Chemical Society. D) Bioluminescence images and the corresponding bioluminescencei ntensity of 24 (20 mm)i ncubated with various concentrationso fGGT.R eprinted with permissionf rom ref [49]…”

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confidence: 99%

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Recent Advances in the Development of Optical Imaging Probes for γ‐Glutamyltranspeptidase

Luo

2018

ChemBioChem

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γ-Glutamyltranspeptidase (GGT) is a cell-membrane-bound protease that participates in cellular glutathione and cysteine homeostasis, which are closely related to many physiological and pathological processes. The accurate measurement of GGT activity is useful for the early diagnosis of diseases. In the past few years, many efforts have been made to build optical imaging probes for the detection of GGT activity both in vitro and in vivo. In this Minireview, recent advances in the development of various optical imaging probes for GGT, including activatable fluorescence probes, ratiometric fluorescence probes, and activatable bioluminescence probes, are summarized. This review starts from the instruction of the GGT enzyme and its biological functions, followed by a discussion of activatable fluorescence probes that show off-on fluorescence in response to GGT. GGT-activatable two-photon fluorescence imaging probes with improved imaging depth and spatial resolution are also discussed. Ratiometric fluorescence probes capable of accurately reporting on GGT levels through a self-calibration mechanism are discussed, followed by describing GGT-activatable bioluminescence probes that can offer a high signal-to-background ratio to detect GGT in living mice. Finally, current challenges and further perspectives for the development of molecular imaging probes for GGT are addressed.

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Section: Bioluminescencei Maging Probes For Ggtmentioning

confidence: 96%

Section: Bioluminescence Imaging Probes For Ggtmentioning

confidence: 99%

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Recent Advances in the Development of Optical Imaging Probes for γ‐Glutamyltranspeptidase

Luo

2018

ChemBioChem

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“…† The HPLC purity of compound 2 to compound (GCPA) 2 are 97%, 97%, 98%, 95% and 97%, respectively. The chemical structures of all the new compounds were further veried by 1 H NMR, 13 C NMR and 19 F NMR (see Fig. S11-S21 in ESI †).…”

Section: Chemistry and Radiochemistrymentioning

confidence: 99%

A fluorine-18 labeled radiotracer for PET imaging of γ-glutamyltranspeptidase in living subjects

Gao

Miao

et al. 2021

RSC Adv.

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“…The earlier cancer is discovered, the greater the likelihood of successful treatment. Therefore, effective diagnosis for early cancer is of great importance [ 5 ]. Despite recent advances in traditional clinical diagnostic techniques (including magnetic resonance imaging, ultrasound imaging, computed tomography and positron emission tomography), the accuracy and sensitivity of diagnosis are still poor during the early stages of cancer, when the tumor is only a few cells in size [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Homologous Gold Nanoparticles and Nanoclusters Composites with Enhanced Surface Raman Scattering and Metal Fluorescence for Cancer Imaging

Yan

Duan

et al. 2018

Nanomaterials

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A large number of deaths from cancer can be attributed to the lack of effective early-stage diagnostic techniques. Thus, accurate and effective early diagnosis is a major research goal worldwide. With the unique phenomenon of localized surface plasmon resonance (LSPR), plasmonic nanomaterials have attracted considerable attention for applications in surface-enhanced Raman scattering (SERS) and metal-enhanced fluorescence (MEF). Both SERS and MEF are ultra-sensitive methods for the detection and identification of early tumor at molecular level. To combine the merits of the fast and accurate imaging of MEF and the stable and clear imaging of SERS, we propose a novel dual functional imaging nanoprobe based on gold nanoparticles and gold nanocluster composites (denoted AuNPC-RGD). The gold nanoparticles are used as LSPR substrates to realized enhancement of Raman or fluorescence signal, while the gold nanoclusters serve as a fluorophore for MEF imaging, and exhibit better biocompatibility and stability. Furthermore, target molecule of cyclic Arg-Gly-Asp (cRGD) is incorporated into the composite to improve delivery efficiency, selectivity and imaging accuracy. These integrated properties endow AuNPC-RGD composites with outstanding biocompatibility and excellent imaging abilities, which could be used to achieve accurate and effective diagnosis for early cancer.

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An ultrasensitive bioluminogenic probe of γ-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis

Cited by 26 publications

References 28 publications

Recent Advances in the Development of Optical Imaging Probes for γ‐Glutamyltranspeptidase

Recent Advances in the Development of Optical Imaging Probes for γ‐Glutamyltranspeptidase

A fluorine-18 labeled radiotracer for PET imaging of γ-glutamyltranspeptidase in living subjects

Homologous Gold Nanoparticles and Nanoclusters Composites with Enhanced Surface Raman Scattering and Metal Fluorescence for Cancer Imaging

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