An ultrasensitive lateral flow immunoassay platform for foodborne biotoxins and pathogenic bacteria based on carbon-dots embedded mesoporous silicon nanoparticles fluorescent reporter probes

Wang, Yuhui; Deng, Chen; Qian, S.‐B.; Li, Hui; Fu, Pan; Zhou, Hualan; Jian, Zheng

doi:10.1016/j.foodchem.2022.133970

Cited by 45 publications

(21 citation statements)

References 39 publications

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“…( d ) Illustration of lateral flow immunoassay platform based on carbon−dots embedded mesoporous silicon nanoparticles fluorescent reporter probes. Adapted with permission from [ 10 , 12 , 23 , 24 ]. Copyright 2018 and 2020, American Chemical Society.…”

Section: Figurementioning

confidence: 99%

Nanosphere Structures Using Various Materials: A Strategy for Signal Amplification for Virus Sensing

Muttaqien

Khoris

Pambudi³

et al. 2022

Sensors

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Nanomaterials have been explored in the sensing research field in the last decades. Mainly, 3D nanomaterials have played a vital role in advancing biomedical applications, and less attention was given to their application in the field of biosensors for pathogenic virus detection. The versatility and tunability of a wide range of nanomaterials contributed to the development of a rapid, portable biosensor platform. In this review, we discuss 3D nanospheres, one of the classes of nanostructured materials with a homogeneous and dense matrix wherein a guest substance is carried within the matrix or on its surface. This review is segmented based on the type of nanosphere and their elaborative application in various sensing techniques. We emphasize the concept of signal amplification strategies using different nanosphere structures constructed from a polymer, carbon, silica, and metal–organic framework (MOF) for rendering high-level sensitivity of virus detection. We also briefly elaborate on some challenges related to the further development of nanosphere-based biosensors, including the toxicity issue of the used nanomaterial and the commercialization hurdle.

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Section: Figurementioning

confidence: 99%

Nanosphere Structures Using Various Materials: A Strategy for Signal Amplification for Virus Sensing

Muttaqien

Khoris

Pambudi³

et al. 2022

Sensors

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“…The development of MSNs with intrinsic luminescent centers has become a controversial topic . Fluorescent carbon dots (CDs), as inorganic fluorophores with a zero-carbon microstructure, offer better chemical stability, excellent water solubility, and minimal cytotoxicity. − With the use of fluorescent CD loading, it is anticipated that stable pesticide absorption, transport, and distribution monitoring will be possible in soybean plants.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Wang

Zhao

et al. 2023

J. Agric. Food Chem.

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Prothioconazole (PTC) has been widely utilized for plant fungal disease control, but its metabolite prothioconazoledesthio (PTC-d) exhibits reproductive toxicity. In the present study, carbon quantum dot (CQD)-modified fluorescent doublehollow shelled mesoporous silica nanoparticles (FL-MSNs) loaded with PTC, referred to as PTC@FL-MSNs, were constructed with an average size of 369 nm and a loading capacity of 28.1 wt %, which could increase the antifungal efficiency of PTC. In addition, upright fluorescence microscope and UPLC-MS/MS studies showed that PTC@FL-MSNs could be effectively transported via root uptake and foliar spray in soybean plants. Compared to a 30% PTC dispersible oil suspension agent, the PTC@FL-MSN treatment group showed higher concentrations (leaves: 0.50 > 0.48 mg/kg), longer half-lives for degradation (leaves: 3.62 > 3.21 d; roots: 3.39 > 2.82 d), and fewer metabolites. These findings suggest that sustained pesticide release and toxicity reduction are potential applications for PTC nanofungicide delivery technology.

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“…The carbon dot-based material is considered to be promising and competitive label probe candidates. However, few reports have used carbon dots alone as LFA applications due to the weak fluorescence intensity of single carbon dots ( Wang et al, 2022a ). Many researchers have utilized silicon spheres as matrix to obtain the stable luminescent composites ( Wang et al, 2022a ).…”

Section: Introductionmentioning

confidence: 99%

“…However, few reports have used carbon dots alone as LFA applications due to the weak fluorescence intensity of single carbon dots ( Wang et al, 2022a ). Many researchers have utilized silicon spheres as matrix to obtain the stable luminescent composites ( Wang et al, 2022a ). Unfortunately, the integration of carbon dots into the nanosphere process usually requires precise control of the reaction conditions to avoid the aggregation of carbon dots, which may affect their fluorescence properties due to aggregation-caused quenching (ACQ) ( Xu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform for the detection of SARS-CoV-2-specific IgM and IgG by the aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein (SSP). The aggregation-induced emission carbon dots (AIE-CDs) are one of the best prospect fluorescent probe materials for exhibiting high emission efficiency in both aggregate and solid states. The AIE-CDs were synthesized and displayed dual fluorescence emission, which provides a new perspective for the design of a high sensitivity testing system. In this work, the novel LFA platform adopted the AIE carbon dots, which are used to detect SARS-CoV-2-specific IgM and IgG conveniently. Furthermore, this sensor had a low LOD of 100 pg/ml. Therefore, this newly developed strategy has potential applications in the areas of public health for the advancement of clinical research.

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An ultrasensitive lateral flow immunoassay platform for foodborne biotoxins and pathogenic bacteria based on carbon-dots embedded mesoporous silicon nanoparticles fluorescent reporter probes

Cited by 45 publications

References 39 publications

Nanosphere Structures Using Various Materials: A Strategy for Signal Amplification for Virus Sensing

Nanosphere Structures Using Various Materials: A Strategy for Signal Amplification for Virus Sensing

Bioactivity, Uptake, and Distribution of Prothioconazole Loaded on Fluorescent Double-Hollow Shelled Mesoporous Silica in Soybean Plants

Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots

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