An ultrasensitive sandwich-type electrochemical aptasensor using silver nanoparticle/titanium carbide nanocomposites for the determination of Staphylococcus aureus in milk

Hui, Yuanyuan; Peng, Haishuai; Zhang, Fuxin; Zhang, Lei; Liu, Yufang; Jia, Rong; Song, Yuxuan; Wang, Bini

doi:10.1007/s00604-022-05349-8

Cited by 13 publications

(4 citation statements)

References 55 publications

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“…aureus as low as 10 CFU mL –1 could also be detected. Compared with our previous MIPs-based label-free method, , and most of the reported sandwich electrochemical sensor, ,,, this assay showed higher sensitivity in milk samples, and meanwhile avoided the complicated and time-consuming pretreatment steps such as centrifuge, filtration, etc.…”

Section: Resultsmentioning

confidence: 90%

“…The most critical component for a sensor is the recognition element (referred to as receptor), which defines its selectivity and sensitivity. Among various recognition elements (e.g., antibody, , aptamer, antimicrobial peptides, , antibiotic, molecularly imprinted polymer , and boronate-affinity compound), antibodies are the dominant receptors owing to their distinguished affinity and specificity. However, natural antibodies are still limited by their high cost, poor stability, and ethical issues in animal experiments. , Thus, developing synthetic receptors as antibody substitutes holds significant importance.…”

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confidence: 99%

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Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

et al. 2023

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Sensitive and rapid detection of pathogenic bacteria is essential for effective source control and prevention of microbial infectious diseases. However, it remains a substantial challenge to rapidly detect bacteria at the single-cell level. Herein, we present an electrochemical sandwich sensor for highly selective and ultrasensitive detection of a single bacterial cell based on dual recognition by the bacteria-imprinted polymer film (BIF) and aptamer. The BIF was used as the capture probe, which was in situ fabricated on the electrode surface within 15 min via electropolymerization. The aptamer and electroactive 6-(Ferrocenyl)hexanethiol cofunctionalized gold nanoparticles (Au@Fc-Apt) were employed as the signal probe. Once the target bacteria were anchored on the BIF-modified electrode, the Au@Fc-Apt was further specifically bound to the bacteria, generating enhanced current signals for ultrasensitive detection of Staphylococcus aureus down to a single cell in phosphate buffer solution. Even in the complex milk samples, the sensor could detect as low as 10 CFU mL–1 of S. aureus without any complicated pretreatment except for 10-fold dilution. Moreover, the current response to the target bacteria was hardly affected by the coexisting multiple interfering bacteria, whose number is 30 times higher than the target, demonstrating the excellent selectivity of the sensor. Compared with most reported sandwich-type electrochemical sensors, this assay is more sensitive and more rapid, requiring less time (1.5 h) for the sensing interface construction. By virtue of its sensitivity, rapidity, selectivity, and cost-effectiveness, the sensor can serve as a universal detection platform for monitoring pathogenic bacteria in fields of food/public safety.

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Section: Resultsmentioning

confidence: 90%

mentioning

confidence: 99%

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

et al. 2023

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“…In this electrochemical platform, HCR and Nb.BbvCI-assisted DNA walkers were used to achieve signal amplification, which demonstrated excellent analytical performance in the range of 0.01–10000 pg mL −1 , with detection limits as low as 3.3 fg mL −1 . In another study, Hui et al [ 157 ] designed a sandwich-type electrochemical sensor based on AgNPs@Ti 3 C 2 nanocomposites to detect Staphylococcus aureus in milk, where the self-assembled aptamer acts as a signal probe immobilized on CuO/GR nanocomposites by π−π stacking. The bacterial recoveries monitored by this sensor ranged between 92.64% and 109.58%, providing a new approach to the detection of pathogenic bacteria in food bioprocess monitoring.…”

Section: Nanomaterials For Enzyme Immobilizationmentioning

confidence: 99%

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Sun,

Wei,

Zhang

et al. 2023

Biosensors

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Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems, and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilization by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal–organic frameworks, carbon nanotubes, and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors.

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“…play a critical role. Various receptors, including antibodies, aptamers, phages, and carbohydrates, can be employed to target bacteria [ 19 , 20 , 21 , 22 , 23 ]. Among these, antibodies are the most commonly used recognition elements due to their exceptional selectivity and binding affinity.…”

Section: Introductionmentioning

confidence: 99%

A Novel Dual Bacteria-Imprinted Polymer Sensor for Highly Selective and Rapid Detection of Pathogenic Bacteria

Xu,

Lin,

Wang

et al. 2023

Biosensors

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The rapid, sensitive, and selective detection of pathogenic bacteria is of utmost importance in ensuring food safety and preventing the spread of infectious diseases. Here, we present a novel, reusable, and cost-effective impedimetric sensor based on a dual bacteria-imprinted polymer (DBIP) for the specific detection of Escherichia coli O157:H7 and Staphylococcus aureus. The DBIP sensor stands out with its remarkably short fabrication time of just 20 min, achieved through the efficient electro-polymerization of o-phenylenediamine monomer in the presence of dual bacterial templates, followed by in-situ template removal. The key structural feature of the DBIP sensor lies in the cavity-free imprinting sites, indicative of a thin layer of bacterial surface imprinting. This facilitates rapid rebinding of the target bacteria within a mere 15 min, while the sensing interface regenerates in just 10 min, enhancing the sensor’s overall efficiency. A notable advantage of the DBIP sensor is its exceptional selectivity, capable of distinguishing the target bacteria from closely related bacterial strains, including different serotypes. Moreover, the sensor exhibits high sensitivity, showcasing a low detection limit of approximately 9 CFU mL−1. The sensor’s reusability further enhances its cost-effectiveness, reducing the need for frequent sensor replacements. The practicality of the DBIP sensor was demonstrated in the analysis of real apple juice samples, yielding good recoveries. The integration of quick fabrication, high selectivity, rapid response, sensitivity, and reusability makes the DBIP sensor a promising solution for monitoring pathogenic bacteria, playing a crucial role in ensuring food safety and safeguarding public health.

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An ultrasensitive sandwich-type electrochemical aptasensor using silver nanoparticle/titanium carbide nanocomposites for the determination of Staphylococcus aureus in milk

Cited by 13 publications

References 55 publications

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

A Novel Dual Bacteria-Imprinted Polymer Sensor for Highly Selective and Rapid Detection of Pathogenic Bacteria

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