An ultrastructural study of connective tissue in mollusc integument: II. Gastropoda

Bairati, A; Comazzi, M.; Gioria, M

doi:10.1054/tice.2001.0197

Cited by 13 publications

(17 citation statements)

References 9 publications

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“…The basic cellular composition of all skin epithelia in M. crenulata follows the general pattern for gastropods (reviewed by Davies & Hawkins ), and the basal laminae are like those described previously in other molluscs (Pedersen ; Bairati et al. ; Corbetta et al. ).…”

Section: Discussionsupporting

confidence: 67%

“…The biochemical analysis of the secreted mucous coatings may progress faster than our understanding of the role of the cells secreting these gels. The basic cellular composition of all skin epithelia in M. crenulata follows the general pattern for gastropods (reviewed by Davies & Hawkins 1998), and the basal laminae are like those described previously in other molluscs (Pedersen 1991;Bairati et al 2001;Corbetta et al 2002). However, understanding the role of the various types of secretory cells in each epithelium is currently an impossible challenge.…”

Section: Sample Originmentioning

confidence: 61%

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Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

Martin

Bailey

Cohen

et al. 2018

Invertebrate Biology

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Mucus‐secreting epithelia protect marine gastropods from abrasive particles and microbes in seawater. We studied the morphology and function of the epithelium in the giant keyhole limpet Megathura crenulata. All exposed surfaces of the limpets were covered by a mucus‐secreting, simple columnar epithelium, in which most cells on the sole of the foot bore cilia, while the majority of cells on the side of the foot and three mantle regions bore microvilli. None of the coatings had antibacterial properties. The epithelium of the foot was distinct from that of other regions, in that the mucus secreted was used for locomotion and was left behind as the limpet moved. The glycocalyx bound to the microvilli of the mantle cells appeared to be clean in visual and SEM examinations, and attempts to enhance the binding of inert particles and bacteria were unsuccessful. Because studies have shown that standard tissue processing may cause artifactual shrinkage of mucous layers, cryostat sections and additives to standard fixatives were tested, but we found no change in the thickness of the mucous layers of the limpets compared to routinely processed tissues. Sloughing of the glycocalyx in vertebrate systems removes bound microbes, and alterations of the glycocalyx layers are associated with disease conditions. Sloughing of the glycocalyx on limpets was rarely observed. In one case, the outer mantle of the limpet was covered with silt and the glycocalyx appeared to be detaching. This process could be experimentally induced by dousing the outer mantle with talc particles. The types of secretory cells producing the mucus in each region of the skin were characterized using standard histological stains and lectin staining. Because analysis was hampered by their small size, classification of the types of secretory cells was based on TEM descriptions of granule morphology, which allowed for comparison to the secretory cells described from skin of the abalone. The possible roles of the numerous secretions, and the mechanisms of mucus production and loss, are compared to what is known in vertebrate systems and to our relative lack of knowledge regarding invertebrate systems.

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Section: Discussionsupporting

confidence: 67%

Section: Sample Originmentioning

confidence: 61%

Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

Martin

Bailey

Cohen

et al. 2018

Invertebrate Biology

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“…Regarding the gastropod foot some research has focused on histological studies in muscular [8–11] and connective tissues [12]. However, although the epithelium of the foot has received much more attention [13–18], histochemical and ultrarstructural studies on the vestigatropod pedal epithelium are scarce [19, 20].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the AbaloneHaliotis tuberculata(Linnaeus, 1758) (Gastropoda, Haliotidae)

Bravo

Martínez-Zorzano²,

Pérez³

et al. 2012

The Scientific World Journal

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The foot epithelium of the gastropod Haliotis tuberculata is studied by light and electron microscopy in order to contribute to the understanding of the anatomy and functional morphology of the mollusks integument. Study of the external surface by scanning electron microscopy reveals that the side foot epithelium is characterized by a microvillus border with a very scant presence of small ciliary tufts, but the sole foot epithelium bears a dense field of long cilia. Ultrastructural examination by transmission electron microscopy of the side epithelial cells shows deeply pigmented cells with high electron-dense granular content which are not observed in the epithelial sole cells. Along the pedal epithelium, seven types of secretory cells are present; furthermore, two types of subepithelial glands are located just in the sole foot. The presence and composition of glycoconjugates in the secretory cells and subepithelial glands are analyzed by conventional and lectin histochemistry. Subepithelial glands contain mainly N-glycoproteins rich in fucose and mannose whereas secretory cells present mostly acidic sulphated glycoconjugates such as glycosaminoglycans and mucins, which are rich in galactose, N-acetyl-galactosamine, and N-acetyl-glucosamine. No sialic acid is present in the foot epithelium.

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“…These junctions are vital elements of the dermo-epidermal junction whose detailed nature varies between the three molluscan classes studies (Bairati et al, 2000(Bairati et al, , 2001(Bairati et al, , 2003 and overall differs clearly from the dermoepidermal junction of mammals.…”

Section: Discussionmentioning

confidence: 99%

“…The ultrastructure of the epithelial cells is variable in different species of mollusc (Bairati et al, 2000(Bairati et al, , 2001(Bairati et al, , 2003. We carried out a comparative study of the morphology of these cells and, particularly, of their junctional apparatus in the epidermis of species from the three main molluscan classes.…”

mentioning

confidence: 99%

An ultrastructural study of cell junctions and the cytoskeleton in epithelial cells of the molluscan integument

Bairati

Gioria

2007

Journal of Morphology

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Cell junctions and the cytoskeleton of integumental epidermal cells from six bivalves, four gastropods, and two cephalopods were studied by transmission electron microscopy. In all species examined, the junctions in supporting cells presented the following similar pattern: an apical-lateral adhesion belt (occluding junctions were not observed); (b) a lateral complex of septate junctions and smooth septate junctions, with interdigitations between adjacent cells while the gap junctions were not constantly present, and a basal complex with hemidesmosomes, focal contacts, and sometimes basolateral adherent junctions. Desmosomes were never observed. Microfilamentous and microgranular material were present throughout the cells, as bundles of microfilaments within microvilli and the terminal web, within interdigitations, and as cytoplasmic plaques forming part of the adherent junctions, hemidesmosomes, and focal contacts. Bundles of intermediate filaments that originated from basal hemidesmosomes were located close to and oriented parallel with the lateral plasma membrane and terminated within the terminal web. In cells of Aplysia depilans, intermediate filaments converged apically to terminate in hemidesmosome-like structures at the bases of the microvilli. In the cephalopods, hemidesmosomes were never observed and intermediate filaments made direct contact with the basal cell membrane. Some functional interpretations and hypotheses were also discussed.

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An ultrastructural study of connective tissue in mollusc integument: II. Gastropoda

Cited by 13 publications

References 9 publications

Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the AbaloneHaliotis tuberculata(Linnaeus, 1758) (Gastropoda, Haliotidae)

An ultrastructural study of cell junctions and the cytoskeleton in epithelial cells of the molluscan integument

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