An unidentified lipid prevalent in tumors

Abstract

An unidentified lipid was found in five different tumor sources. It was not found in liver, bone marrow or plasma from tumor-bearing animals, nor in the extracellular fluid supporting growth of Ehrlich ascites cells. The polarity of the unidentified component was similar to that of a glyceryl ether diester, and it was isolated in milligram amounts by preparative thinlayer chromatography. Neither methyl esters of fatty acids, vinyl ether diesters nor quinones were found in the structural makeup of this lipid. Thin-layer chromatography of the purified unidentified tumor lipid on Ag-impregnated silica layers revealed two main components of intermediary unsaturation. Saponification of the unidentified tumor lipid, when water washing was omitted, yielded two components that migrated at R(f)'s identical to those of free fatty acids and dihydroxy glyceryl monoethers. Neither acetate-1-(14)C nor palmitic-1-(14)C acid (single injections) was found to be incorporated into the unidentified lipid of a mature rat tumor.

A continuous assay forO‐alkylglycerol monooxygenase (E.C. 1.14.16.5)

“…The antitumor activity of alkyl lysophospholipids {ALP} has been ascribed to their ability to stimulate macrc~ phages (1) and to their cytotoxicity (2-4}. One of the possible explanations for the cytotoxicity is that the tumor cells cannot degrade and therefore they accumulate (5) these lytic compounds because they lack a key enzyme (6}. It is likely that the enzyme(s) cleaving O-alkyl bonds are not present in these cells {6,7}.…”

Tumor lipids: Carbon number distribution of triglycerides and glyceryl ether diesters

“…The occurrence of an unidentified neutral lipid in tumor tissue was first reported by Bollinger (1) and Cheng et al (2) and later confirmed (3,4). The lipid, isolated from Ehrlich ascites cells, was subsequently identified as a glyceryl ether diester (GEDE) (5).…”

The isolation and tentative identification of diacylglyceryl ethers from the walker 256 carcinoma of the rat and a human lymphosarcoma

“…(b) Gas-liquid chromatographic analysis of trimethylsilyl derivatives of the unknown humanlymphosarcoma nonsaponifiable lipid on a QF1 column. Retention times were exactly the same as chimyl (1) and batyl alcohol (2) TMS derivatives.…”