An Update on Biomedical Application of Nanotechnology for Alzheimer’s Disease Diagnosis and Therapy

Panahi, Yunes; Mohammadhosseini, Majid; Abadi, Azam Jafari Najaf; Akbarzadeh, Abolfazl; Mellatyar, Hassan

doi:10.1055/s-0042-112811

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…Alzheimer’s disease (AD) is a deadly chronic neurodegenerative disorder in the world, which contributes to memory loss and cognitive decline in the elderly [1,2]. It is estimated that there are millions of people suffering AD over the world [3]. Many studies reported that one of the causes of AD was the accumulation of amyloid-β (Aβ) fibrillar deposition [4].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Degradation Conditions with PRG, a Polysaccharide from Phellinus ribis, by RSM and the Neuroprotective Activity in PC12 Cells Damaged by Aβ25–35

et al. 2019

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In the previous work, we found PRG, a polysaccharide from Phellinus ribis, exhibited neurotrophic activity. To obtain an active structural unit with lower molecular weight, PRG was degraded to prepare the degraded PRG (DPRG) using ascorbic acid and H2O2. The aim of the paper was to obtain DPRG by optimizing the degradation conditions using response surface methodology (RSM) and to study its protective effects of PC12 cells induced by Aβ25–35. The optimum conditions were as follows; the concentration of H2O2-Vc was 17 mM and degradation temperature was 50 °C; when degradation time was 1.6 h, the experimental response value of PC12 cell viability was 83.4 ± 0.15%, which was in accordance with the predicted value (83.5%). We also studied the protective effects of DPRG against the Aβ25–35-induced neurotoxicity and explored the underlying mechanism. The results showed that treatment with DPRG could attenuate PC12 cells death. The mechanism was relative to the inhibition of cell apoptosis by increasing the MMP level and decreasing the protein expression of cytochrome C (Cytc) in PC12 cells. In conclusion, DPRG with lower molecular weight was obtained successfully. It possessed neuroprotective properties and might be a candidate for neurodegenerative disease treatment.

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