Objective: To describe robot-assisted radical prostatectomy (RARP) and report the short-term outcome of a dog with prostatic cancer treated with RARP. Study design: Case report Animals: Client-owned 6-year-old male-neutered Bernese mountain dog. Methods: Robot-assisted radical prostatectomy was performed with a daVinci Si Surgical System through a transperitoneal approach. An interfascial nervesparing approach was used to preserve the neurovascular bundles and increase the likelihood of postoperative urinary continence. Urinary continence was assessed by placing white cloth bedding in the kennel during hospitalization and by owners' observation at home. Results: The console time was 120 minutes, and the estimated intraoperative blood loss was 30 mL. No intraoperative complications were observed. The urinary catheter was removed after 7 days, at which point the dog urinated normally. Peritoneal carcinomatosis was diagnosed 43 days after surgery, and the dog was euthanized. Conclusion: Robot-assisted radical prostatectomy was successfully completed in a dog with prostatic neoplasia and led to postoperative urinary continence. Clinical significance: This single-case report provides evidence to justify further evaluation of RARP in dogs with prostatic neoplasms. 1 | INTRODUCTION Prostatic cancer has a prevalence of 0.2% to 0.6% in the domestic dog population and affects 13% of dogs with prostatic disease. 1,2 No effective curative treatment modality currently exists. A much longer median survival time was described in dogs with prostatic carcinoma receiving cyclooxygenase (COX) inhibitors vs dogs not receiving any treatment (6.9 vs 0.7 months) in one retrospective study. 3 Whereas treatment effect is indisputable because of the over expression of COX-2 in canine prostatic carcinoma cells, 3 the difference in survival times in that study was most likely biased because there was no randomization in treatment groups. Information on the role of radiation therapy for canine prostatic carcinoma in the veterinary literature remains scarce. In a study in A. Schlake and P. Dell'Oglio share first authorship.