An updated review of the H19 lncRNA in human cancer: molecular mechanism and diagnostic and therapeutic importance

Alipoor, Behnam; Parvar, Seyedeh Nasrin; Sabati, Zolfaghar; Ghaedi, Hamid; Ghasemi, Hassan

doi:10.1007/s11033-020-05695-x

Cited by 58 publications

(47 citation statements)

References 202 publications

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“…LncRNA, as a highly active RNA, has been proved to participate in the genesis and development of a number of tumors [4]. Among them, lncRNA H19 has been found to be abnormally expressed in human malignant tumors and regulates cell proliferation, migration, invasion, antiapoptosis, and epithelial-mesenchymal transition (EMT) through various mechanisms, thus playing a carcinogenic or anticancer role [5][6][7]. H19 has been found to act as a microRNA sponge to indirectly regulate the expression of microRNA downstream target genes thus mediating cancer progression in several cancer types [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

Ding

Yang

2021

BioMed Research International

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Objective. The abnormal expression of LncRNA H19 and miR-140-5p has been linked to ovarian cancer (OC). Whether H19 directly regulates miR-140-5p in ovarian cancer cells has been unclear. In this study, we deeply explored the relationship between H19 and miR-140-5p in ovarian cancer and the mechanism of action in regulating OC progression. Methods. A total of 66 patients with OC admitted to the hospital from June 2017 to June 2019 were selected as the research group (RG), and meanwhile, 60 cases of healthy subjects were selected as the control group (CG). In addition, OC cells and normal ovarian epithelial cells were used to detect H19 and miR-140-5p expression levels and to analyze the effect of H19 on OC cells. The activation of the PI3K/AKT pathway and downstream proteins were analyzed by western blot. Results. H19 was highly expressed while miR-140-5p was lowly expressed in OC patients and cell lines ( P < 0.050 ). The proliferation, invasion, migration ability, and epithelial-mesenchymal transition (EMT) of OC cells were reduced after inhibiting H19 expression, and the apoptosis rate was increased. Transfection of cells with miR-140-5p mimics brought opposite effects. Online prediction and dual-luciferase reporter (DLR) confirmed that H19 directly binds miR-140-5p. Western blot assay indicated overexpression activated the PI3K/AKT signaling pathway in OC cells. Moreover, overexpression promoted tumor growth in nude mice and was suppressed by PI3K inhibitor. Conclusion. LncRNA H19 downregulation of miR-140-5p to activate the PI3K/AKT signaling pathway and promote the proliferation, invasion, migration and EMT of OC.

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Section: Introductionmentioning

confidence: 99%

Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

Ding

Yang

2021

BioMed Research International

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“…McCollough et al [ 3 ] also examined the association between methylation changes at MEG-3 in cord blood in offspring of mothers consuming a pro-inflammatory diet during pregnancy, but no association between maternal intake of a pro-inflammatory dietary pattern and methylation of the DMRs could be found. Miyaso et al [ 16 ] focused on another tumor suppressor gene, namely H19 [ 32 ]. As the authors state, changes in the methylation of the H19 DMR are related to human health, but little is known about the factors that regulate the methylation of H19 DMR.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Lifestyle, Diet and Physical Activity on Epigenetic Changes in the Offspring—A Systematic Review

et al. 2021

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Aims: This systematic review examines the association between maternal lifestyle, diet and physical activity, and epigenetic changes in the offspring. Methods: A literature search was conducted using multiple science databases: PubMed, Embase and Cochrane Library, on 10 March 2021. RCT and Cohort studies in English or Scandinavian languages were included. Exposure variables included diet, lifestyle, meal patterns or physical activity. Studies using dietary supplements as exposure variables were excluded. Outcome variables included were DNA methylation, microRNA or histone changes in placenta, cord blood or offspring. Two independent authors screened, read and extracted data from the included papers. The Cochrane risk-of-bias tool for randomized trials (RoB2) and The Critical Appraisal Skills Program (CASP) Cohort Study Checklist were used to assess risk of bias in the included studies. A qualitative approach was employed due to heterogeneity of exposures and results of the studies. Results: 16 studies and 3617 participants were included in the final analysis. The exposure variables included physical activity, carbohydrate, low glycemic index diet, added sugar, fat, Mediterranean diet and pro-inflammatory diet. The outcome variables identified were differences in DNA methylation and microRNA. Most studies described epigenetic changes in either placenta or cord blood. Genes reported to be methylated were GR, HSD2, IGF-2, PLAG1, MEG-3, H19 and RXRA. However, not all studies found epigenetic changes strong enough to pass multiple testing, and the study quality varied. Conclusion: Despite the variable quality of the included studies, the results in this review suggest that there may be an association between the mother’s lifestyle, diet and level of physical activity during pregnancy and epigenetic changes in the offspring.

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“…Of course, there is a considerable body of existing researches regarding the modulation of H19 to cell cycle and apoptosis through other pathways. H19 inhibits the apoptosis process via the downregulation of proapoptotic factor Bax as well as tumor suppressor factor p53 [26]. Besides, H19 contacts with the 7 BioMed Research International apoptosis process and cell cycle depending on the regulation to miR-138 and SOX4 [27], miR-675 [28], miRNA-107 [29], YAP1 [30], and Ras/Raf/MEK/ERK cascade [31].…”

Section: Discussionmentioning

confidence: 99%

LncRNA H19 Upregulation Participates in the Response of Glioma Cells to Radiation

Bing

Jin

2021

BioMed Research International

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Previous studies have indicated that radiation resistance of glioma is one of the leading causes of radiotherapy failure. Mounting evidence suggests that long non-coding RNA (lncRNA) plays an important role in regulating radiosensitivity of cancer cells via implicating in various cell processes. However, the underlying mechanisms remain unclear and need further study, especially at the molecular level. We found that the expression level of lncRNA H19 was elevated by radiation, and then, the modulation of H19 affected the resistant of glioma cells to X-rays. Dual-luciferase reporter analyses showed that H19 was transcriptionally activated by CREB1 in glioma cells after irradiation. In addition, both flow cytometry and 5-ethynyl-2 ′ -deoxyuridine (EdU) assay suggested that H19 was involved in the cell cycle arrest, apoptosis, and DNA synthesis to modulate the radiation response of glioma cells and influenced their radioresistance. Therefore, H19 might play a crucial role in enhancing the radioresistance of glioma.

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An updated review of the H19 lncRNA in human cancer: molecular mechanism and diagnostic and therapeutic importance

Cited by 58 publications

References 202 publications

Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

The Impact of Lifestyle, Diet and Physical Activity on Epigenetic Changes in the Offspring—A Systematic Review

LncRNA H19 Upregulation Participates in the Response of Glioma Cells to Radiation

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