An XPS study on the attachment of triethoxsilylbutyraldehyde to two titanium surfaces as a way to bond chitosan

Martin, Holly J.; Schulz, Kirk H.; Bumgardner, Joel D.; Walters, Keisha B.

doi:10.1016/j.apsusc.2008.01.066

Cited by 63 publications

(35 citation statements)

References 38 publications

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“…Untreated surfaces presented three C 1s peaks (284.8, 285.7 and 288.1 eV, Table 5) associated to hydrocarbon contamination (CHx, C-O and C=O bonds) [33][34][35]. After NaOH activation, total carbon presence was lower, and C 1s high resolution spectra was fitted to two peaks at 284.8 and 288.8 eV which can be attributed to C-H and C=O respectively [33][34][35]. These results indicate that Ti_N surfaces were cleaner after the NaOH treatment.…”

Section: Discussionmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

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Section: Discussionmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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“…Gadenne et al used also this type of covalent binding in order to graft polysaccharide via a silane onto titanium surface [54]. In the literature, chitosan grafting on Ti can be performed via imide bond formation using TESBA [22] or APTES associated with glutaraldehyde [25].…”

Section: Xps Analysismentioning

confidence: 97%

“…Various strategies such as electrodeposition of alginate/chitosan layer-by-layer on substrates [19], the use of catechol groups [20] and dopamine-glutaraldehyde system [21] have been already reported to bond chitosan to titanium. Another widespread approach consists in using organosilane as coupling agent such as triethoxysilylbutyraldehyde (TESBA) [22,23], triethoxysilylpropylsuccinic anhydride (TESPSA) [24] or 3-aminopropyltriethoxysilane (APTES) associated with glutaraldehyde [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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“…However, the attachment, stability and bioactivity maintenance of chitosan coatings at implant surfaces under drastic environments such as the oral cavity have proven to be highly influenced by different factors such as pH value variations [15,16]. To improve the strength of the covalent adhesion bindings and consequently the immobilization of chitosan molecules on the Ti surfaces, organic coupling agents such as glutaraldehyde [17,18] or silanated intermediaries such as 3-aminopropyltriethoxysilane (APTES) [19,20] or triethoxysylilbutyraldehyde (TESBA) molecules [21] have been employed. Currently, a novel chemical functionalization of Ti surfaces by peptide bonds has provided greater mechanical and immobilization properties of chitosan coatings [22].…”

Section: Introductionmentioning

confidence: 99%

Acidic pH resistance of grafted chitosan on dental implant

et al. 2014

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Over the last decade, access to dental care has increasingly become a service requested by the population, especially in the case of dental implants. However, the major cause of implant failure is an inflammatory disease: peri-implantitis. Currently, the adhesion strength of antibacterial coatings at implant surfaces remains a problem to solve. In order to propose a functionalized implant with a resistant antibacterial coating, a novel method of chitosan immobilization at implant surface has been investigated. Functionalization of the pre-active titanium (Ti) surface was performed using triethoxysilylpropyl succinic anhydride (TESPSA) as a coupling agent which forms a stable double peptide bond with chitosan. The chitosan presence and the chemical resistibility of the coating under acid pH solutions (pH 5 and pH 3) were confirmed by FTIR-ATR and XPS analyses. Furthermore, peel test results showed high adhesive resistance of the TESPSA/chitosan coating at the substrate. Cytocompatibility was evaluated by cell morphology with confocal imaging. Images showed healthy morphology of human gingival fibroblasts (HGF-1). Finally, the reported method for chitosan immobilization on Ti surface via peptide bindings allows for the improvement of its adhesive capacities and resistibility while maintaining its cytocompatibility. Surface functionalization using the TESPSA/chitosan coupling method is noncytotoxic and stable even in drastic environments as found in oral cavity, thus making it a valuable candidate for clinical implantology applications.

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An XPS study on the attachment of triethoxsilylbutyraldehyde to two titanium surfaces as a way to bond chitosan

Cited by 63 publications

References 38 publications

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Acidic pH resistance of grafted chitosan on dental implant

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