We demonstrate fluorescence microscopy of individual fermionic potassium atoms in a 527-nmperiod optical lattice. Using electromagnetically induced transparency (EIT) cooling on the 770.1-nm D1 transition of 40 K, we find that atoms remain at individual sites of a 0.3-mK-deep lattice, with a 1/e pinning lifetime of 67(9) s, while scattering ∼ 10 3 photons per second. The plane to be imaged is isolated using microwave spectroscopy in a magnetic field gradient, and can be chosen at any depth within the three-dimensional lattice. With a similar protocol, we also demonstrate patterned selection within a single lattice plane. High resolution images are acquired using a microscope objective with 0.8 numerical aperture, from which we determine the occupation of lattice sites in the imaging plane with 94(2)% fidelity per atom. Imaging with single-atom sensitivity and addressing with single-site accuracy are key steps towards the search for unconventional superfluidity of fermions in optical lattices, the initialization and characterization of transport and non-equilibrium dynamics, and the observation of magnetic domains.Ultracold fermionic atoms in an optical lattice realize an impurity-free analog of electrons in crystalline materials, with full control of parameters such as interaction strength, dimensionality, and tunneling [1,2]. Furthermore, ultracold systems can study many-body physics in scenarios currently inaccessible to materials, such as gauge fields equivalent to thousands of Tesla [3][4][5], interactions at the unitary limit [6], and quantum manybody physics far from equilibrium [7]. With sufficient control and probes, these experiments can be considered analog quantum simulations [8,9]. However, two important tools have been lacking: imaging and addressing fermionic atoms at the single-site and single-atom level [9]. When applied to bosonic atoms, these tools have already been dramatically successful [10][11][12][13][14][15][16][17][18][19][20].High-resolution imaging and manipulation of ultracold fermions solves several outstanding problems at once. First, in-situ spatial probes directly reveal the order parameter of insulating phases, magnetic domain formation, and other correlations inaccessible in time-of-flight imaging [13,14,19]. Second, an ensemble of density distributions provides a direct measure of entropy [13,14], extending thermometry of lattice fermions [21]. Third, manipulation of atoms with single-site precision can initiate dynamics [15,16], project or remove disorder [14], and selectively remove high entropy atoms to perform in-situ cooling [22,23].This year, five research groups have succeeded in imaging single fermions in an optical lattice: three using Raman sideband cooling [24][25][26] and two using EIT cooling [27], including the results reported in this Article. Our approach is distinguished by a unique imaging configuration, and takes a further step by implementing threedimensional spatial addressing, which is used here for selective removal of atoms from the lattice. Figure 1 ill...