Anaerobic Biodegradation of Hydrocarbons: Metagenomics and Metabolomics

Gieg, Lisa M.; Toth, Courtney R. A.

doi:10.1007/978-3-319-50433-9_16

Cited by 2 publications

(2 citation statements)

References 166 publications

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“…Anaerobic biodegradation of hydrocarbons is a significant process that occurs in many environments (Wawrik et al, 2012; Gründger et al, 2015; Gieg and Toth, 2016; Laso-Pérez et al, 2016) and may be an important process in the deep-water communities of the Caspian Sea. Anaerobic hydrocarbon degradation is most frequently reported to be slower than aerobic hydrocarbon degradation (Widdel et al, 2010), with biodegradation coefficients of 0.445/d and 0.522/d, respectively (Suarez and Rifai, 1999).…”

Section: Introductionmentioning

confidence: 99%

Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

et al. 2019

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The Caspian Sea, which is the largest landlocked body of water on the planet, receives substantial annual hydrocarbon input from anthropogenic sources (e.g., industry, agriculture, oil exploration, and extraction) and natural sources (e.g., mud volcanoes and oil seeps). The Caspian Sea also receives substantial amounts of runoff from agricultural and municipal sources, containing nutrients that have caused eutrophication and subsequent hypoxia in the deep, cold waters. The effect of decreasing oxygen saturation and cold temperatures on oil hydrocarbon biodegradation by a microbial community is not well characterized. The purpose of this study was to investigate the effect of oxic and anoxic conditions on oil hydrocarbon biodegradation at cold temperatures by microbial communities derived from the Caspian Sea. Water samples were collected from the Caspian Sea for study in experimental microcosms. Major taxonomic orders observed in the ambient water samples included Flavobacteriales , Actinomycetales , and Oceanospirillales . Microcosms were inoculated with microbial communities from the deepest waters and amended with oil hydrocarbons for 17 days. Hydrocarbon degradation and shifts in microbial community structure were measured. Surprisingly, oil hydrocarbon biodegradation under anoxic conditions exceeded that under oxic conditions; this was particularly evident in the degradation of aromatic hydrocarbons. Important microbial taxa associated with the anoxic microcosms included known oil degraders such as Oceanospirillaceae . This study provides knowledge about the ambient community structure of the Caspian Sea, which serves as an important reference point for future studies. Furthermore, this may be the first report in which anaerobic biodegradation of oil hydrocarbons exceeds aerobic biodegradation.

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Section: Introductionmentioning

confidence: 99%

Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

et al. 2019

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“…Several genera known to be implicated in biodegradation of PHCs and various TPH constituents are found above 2% relative abundance in samples from source area (primarily W-15R, W-1, and MW-A1) and downgradient plume (MW-A2) monitoring wells that are absent from the background sample. Genera detected in source area and plume area monitoring wells above background relative abundances and linked to PHC biodegradation include Dechloromonas (Chakraborty et al, 2005), Deinococcus (Liang et al, 2011), Clostridium (Gieg et al, 2014), Geobacillus (Elumalai et al, 2019), Syntrophus (Gieg and Toth, 2019), Zoogloea (Farkas et al, 2015), Enterobacter (Ramasamy et al, 2017), and Petrotoga (Purwasena et al, 2014). In addition to the presence of PHC degraders in the source and plume area, methanogenic microorganisms including those belonging to the Classes Methanobacteria and Methanomicrobia were also found in higher relative abundance in the source and downgradient plume areas above the background suggesting hydrocarbon degradation to methane by methanogens is likely occurring.…”

Section: Assessment Stagementioning

confidence: 99%

Increasing in situ bioremediation effectiveness through field-scale application of molecular biological tools

et al. 2023

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Leveraging the capabilities of microorganisms to reduce (degrade or transform) concentrations of pollutants in soil and groundwater can be a cost-effective, natural remedial approach to manage contaminated sites. Traditional design and implementation of bioremediation strategies consist of lab-scale biodegradation studies or collection of field-scale geochemical data to infer associated biological processes. While both lab-scale biodegradation studies and field-scale geochemical data are useful for remedial decision-making, additional insights can be gained through the application of Molecular Biological Tools (MBTs) to directly measure contaminant-degrading microorganisms and associated bioremediation processes. Field-scale application of a standardized framework pairing MBTs with traditional contaminant and geochemical analyses was successfully performed at two contaminated sites. At a site with trichloroethene (TCE) impacted groundwater, framework application informed design of an enhanced bioremediation approach. Baseline abundances of 16S rRNA genes for a genus of obligate organohalide-respiring bacteria (i.e., Dehalococcoides) were measured at low abundances (101–102 cells/mL) within the TCE source and plume areas. In combination with geochemical analyses, these data suggested that intrinsic biodegradation (i.e., reductive dechlorination) may be occurring, but activities were limited by electron donor availability. The framework was utilized to support development of a full-scale enhanced bioremediation design (i.e., electron donor addition) and to monitor remedial performance. Additionally, the framework was applied at a second site with residual petroleum hydrocarbon (PHC) impacted soils and groundwater. MBTs, specifically qPCR and 16S gene amplicon rRNA sequencing, were used to characterize intrinsic bioremediation mechanisms. Functional genes associated with anaerobic biodegradation of diesel components (e.g., naphthyl-2-methyl-succinate synthase, naphthalene carboxylase, alkylsuccinate synthase, and benzoyl coenzyme A reductase) were measured to be 2–3 orders of magnitude greater than unimpacted, background samples. Intrinsic bioremediation mechanisms were determined to be sufficient to achieve groundwater remediation objectives. Nonetheless, the framework was further utilized to assess that an enhanced bioremediation could be a successful remedial alternative or complement to source area treatment. While bioremediation of chlorinated solvents, PHCs, and other contaminants has been demonstrated to successfully reduce environmental risk and reach site goals, the application of field-scale MBT data in combination with contaminant and geochemical data analyses to design, implement, and monitor a site-specific bioremediation approach can result in more consistent remedy effectiveness.

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Anaerobic Biodegradation of Hydrocarbons: Metagenomics and Metabolomics

Cited by 2 publications

References 166 publications

Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

Increasing in situ bioremediation effectiveness through field-scale application of molecular biological tools

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