Anaerobic growth of halobacteria.

Hartmann, Rainer; Sickinger, Hans-Dieter; Oesterhelt, Dieter

doi:10.1073/pnas.77.7.3821

Cited by 189 publications

(135 citation statements)

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“…Formation of gaseous products from nitrate was detected by the presence of gas bubbles in Durham tubes. Anaerobic growth in the presence of either nitrate or arginine was tested as described previously (Hartmann et al, 1980 ;Mancinelli & Hochstein, 1986). Production of acids from sugars was examined in SG medium supplemented with 0n5 % of the sugars tested.…”

Section: Collection Of Samples and Isolation Of Halophilic Archaeamentioning

confidence: 99%

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Montalvo‐Rodríguez¹,

López‐Garriga²,

Vreeland³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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An extremely halophilic Archaeon belonging to the order Halobacteriales was isolated from the solar salterns of Cabo Rojo, Puerto Rico. The organism, designated strain PR5 T , is rod-shaped, non-motile and requires at least 12 % (w/v) NaCl to grow. The strain is highly thermotolerant : its temperature optimum is 50 SC and growth is possible up to 60 SC. Polar lipid analysis revealed the presence of the bis-sulfated glycolipid S 2 -DGD-1 as sole glycolipid and the absence of the glycerol diether analogue of phosphatidylglycerosulfate. Both C 20 ,C 20 and C 20 ,C 25 core lipids are present. The GMC content of the DNA is 63 3 mol %. According to 16S rDNA sequence data, strain PR5 T is closely related to the representatives of the genera Haloterrigena and Natrinema, but on the basis of its phenotypic properties, 16S rDNA sequence and DNA-DNA hybridization studies, strain PR5 T cannot be assigned to any of the recognized species within these genera. On the basis of its polar lipid composition, the isolate has been assigned to the genus Haloterrigena. The creation of a new species, Haloterrigena thermotolerans, is therefore proposed to accommodate this isolate. The type strain is strain PR5 T (l DSM 11552 T l ATCC 700275 T ).

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Section: Collection Of Samples and Isolation Of Halophilic Archaeamentioning

confidence: 99%

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Montalvo‐Rodríguez¹,

López‐Garriga²,

Vreeland³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…Although this capability has been demonstrated for haloarchaeal bacteriorhodopsins (18,19,26,27), PR-based photophosphorylation has not been demonstrated previously. Our data demonstrate that illumination of cells expressing a native marine bacterial PR photosystem generates a proton-motive force that does indeed drive cellular ATP synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…5A) and extent of ␤␣-to-␣␤ isomerization around C17 and C21 for C 29 and C 30 homologues (moretane/hopane; Fig. 5C and E), as well as ratios of C [27][28][29] ␣␤␤ to ␣␣␣ steranes (Fig. 8D) and epimerizaton around C20 (Fig.…”

Section: Composition Of Bitumens I and Iimentioning

confidence: 99%

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Molecular biogeochemistry of modern and ancient marine microbes

Waldbauer¹

2010

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Biological activity has shaped the surface of the earth in numerous ways, but life's most pervasive and persistent global impact has been the secular oxidation of the surface environment. Through primary production -the biochemical reduction of carbon dioxide to synthesize biomass -large amounts of oxidants such as molecular oxygen, sulfate and ferric iron have accumulated in the ocean, atmosphere and crust, fundamentally altering the chemical environment of the earth's surface. This thesis addresses aspects of the role of marine microorganisms in driving this process. In the first section of the thesis, biomarkers (hydrocarbon molecular fossils) are used to investigate the early history of microbial diversity and biogeochemistry. Molecular fossils from the Transvaal Supergroup, South Africa, document the presence in the oceans of a diverse microbiota, including eukaryotes, as well as oxygenic photosynthesis and aerobic biochemistry, by ca. 2.7Ga. Experimental study of the oxygen requirements of steroid biosynthesis suggests that sterane biomarkers in late Archean rocks are consistent with the persistence of microaerobic surface ocean environments long before the initial oxygenation of the atmosphere. In the second part, using Prochlorococcus (a marine cyanobacterium that is the most abundant primary producer on earth today) as a model system, we explored how microbes use the limited nutrient resources available in the marine environment to make the protein catalysts that enable primary production. Quantification of the Prochlorococcus proteome over the diel cell-division cycle reveals that protein abundances are distinct from transcript-level dynamics, and that small temporal shifts in enzyme levels can redirect metabolic fluxes. This thesis illustrates how molecular techniques can contribute to a systems-level understanding of biogeochemical processes, which will aid in reconstructing the past of, and predicting future change in, earth surface environments. ACKNOWLEDGMENTSI have incurred many debts of gratitude and appreciation over the course of this work. None are greater than those to my advisors, Penny Chisholm and Roger Summons, who allowed and enabled me to pursue such a wide range of scientific interests and offered their help and advice every step of the way. I have been very lucky to have had the chance to learn so much during my Ph.D., and none of that would have been possible without their guidance and support.I have also had the great fortune to know and work with all the members of the Chisholm and Summons labs, who have made this an enjoyable and rewarding place to be. I am especially grateful to Laura Sherman for her skill and dedication in analyzing biomarkers in the late Archean cores -it was truly getting blood from a stone. I was lucky to collaborate with Sébastien Rodrigue, whose expertise and collegiality made the diel experiment even more fruitful than I could have hoped. IntroductionThe coevolution of life and earth surface environments is central to the history and functionin...

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“…Therefore, it is not surprising that representatives of the Halobacteriales have developed various modes of energy generation under anaerobic conditions, including denitrification (Mancinelli & Hochstein, 1986), fermentation of arginine (Hartmann et al, 1980), and use of the lightdriven proton pump, bacteriorhodopsin.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic growth of halophilic archaeobacteria by reduction of fumarate

Oren

1991

Journal of General Microbiology

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A number of strains of halophilic archaeobacteria of the genera Halobacterium and Haloferax were able to grow anaerobically using fumarate as electron acceptor. The species showing the best anaerobic growth with fumarate were Haloferax volcanii and Haloferax denitrijicans. The two Haloarcula species tested did not show anaerobic growth enhancement with fumarate. During anaerobic growth of Haloferax trolcanii in the presence of fumarate, succinate accumulated in the medium with a stoichiometry of only 0-16-0-23 mmol succinate per mmol fumarate consumed; this can be explained by the use of succinate for assimilatory purposes. The ability to reduce fumarate to succinate did not correlate with the ability to grow anaerobically using nitrate, dimethylsulphoxide or trimethylamine N-oxide as terminal electron acceptors. Anaerobic respiration with fumarate as electron acceptor supplies the halophilic archaeobacteria with an additional mode of energy generation in the absence of molecular oxygen.

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Anaerobic growth of halobacteria.

Cited by 189 publications

References 10 publications

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Molecular biogeochemistry of modern and ancient marine microbes

Anaerobic growth of halophilic archaeobacteria by reduction of fumarate

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