Anaerobic growth of the haloalkaliphilic denitrifying sulfur-oxidizing bacterium Thialkalivibrio thiocyanodenitrificans sp. nov. with thiocyanate

Sorokin, Dimitry Y.; Tourova, T. P.; Антипов, А. Н.; Muyzer, Gerard; Kuenen, J.G.

doi:10.1099/mic.0.27015-0

Cited by 101 publications

(78 citation statements)

References 28 publications

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“…Kinetic constants were higher in MM for the lowest initial concentrations of thiocyanate, whereas the opposite behaviour was observed for concentrations higher than 4,000 mg L -1 . Degradation rates between 1 and 87 mg (Lh) -1 were reported for the biodegradation of thiocyanate by specific microorganisms [6,18,19,24] similar to the degradation rates obtained in this work. In fact, only Acremonium strictum showed higher removal rates [19].…”

Section: Utilization Of Nitrogen and Sulphur From Thiocyanatesupporting

confidence: 73%

“…According to studies that focused on thiocyanate biodegradation, 2,500 mg L -1 of thiocyanate were biodegraded in 120 hrs with the co-culture Klebsiella pneumoniae and Ralstonia sp [6], 3,395 mg SCN -L -1 were removed after 7 days by Thiobacillus thioparus THI115 [18] and 970 mg SCN -L -1 were eliminated by Thioalkalivibrio thiocyanodenitrificans [24] after 30-40 days. Considering these results, our system is more efficient in terms of the thiocyanate biodegradation rate, especially when the medium is free of C, N and S sources.…”

Section: Utilization Of Nitrogen and Sulphur From Thiocyanatementioning

confidence: 99%

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Conditions and Mechanisms in Thiocyanate Biodegradation

Combarros¹,

Collado²,

Laca³

et al. 2015

JRST

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Presence of thiocyanate (SCN -) is a problem of considerable interest in many industrial wastewaters, where this contaminant often appears accompanied by secondary sources of carbon, nitrogen and/or sulphur. In order to understand the effect of these compounds on the biodegradability of SCN -, this work investigates how the bacterium Pararacoccus thiocyanatus utilises the three elements that form the molecule of thiocyanate and compare this behaviour with those obtained in presence of other sources of N, S and C. Result showed that the bacterium was capable of utilizing thiocyanate as the sole substrate, achieving specific biodegradation rates of approximate 1.20 mg SCN -(mg cell·h) -1 and eliminating initial thiocyanate concentrations up to 5,000 mg L -1 . Experimental data were successfully fitted to a Teisser model, assuming the existence of substrate inhibition obtaining values of μ max of 0.059 h -1 , K s of 790 mg L -1 and K i of 6,520 mg L -1 for free mineral medium. Presence of additional carbon and nitrogen sources implied catabolic repression of the biodegradation of thiocyanate. In this case, only concentrations lower than 3,500 mg L -1 could be treated, obtaining specific degradation rates of around 0.70 mg SCN -·(h·mg cell) -1 . Tessier model values showed a higher maximum specific growth rate (0.344 h -1 ), changing also the values for affinity and inhibition constants (1,150 mg L -1 and 1,730 mg L -1 , respectively).

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Section: Utilization Of Nitrogen and Sulphur From Thiocyanatesupporting

confidence: 73%

Section: Utilization Of Nitrogen and Sulphur From Thiocyanatementioning

confidence: 99%

Conditions and Mechanisms in Thiocyanate Biodegradation

Combarros¹,

Collado²,

Laca³

et al. 2015

JRST

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“…Chemical analysis of sulfur (sulfide, sulfur, thiosulfate and sulfite) and nitrogen (nitrite and ammonium) compounds, and cell protein were performed as described previously (Sorokin et al 2001(Sorokin et al , 2004b. Sulfane atom of polysulfide was analyzed in the same way as free sulfide, i.e.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Dethiobacter alkaliphilus gen. nov. sp. nov., and Desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes

et al. 2008

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“…Differential characteristics of strain XK5 T and other related genera Genera: 1, Woeseia gen. nov. (XK5 T ; data from this study); 2, Thioalkalivibrio (Banciu et al, 2004;Sorokin et al, 2001Sorokin et al, , 2002aSorokin et al, , b, 2003Sorokin et al, , 2004Sorokin et al, , 2012; 3, Natronocella (Sorokin et al, 2007); 4, Thiorhodospira (Bryantseva et al, 1999(Bryantseva et al, , 2010; 5, Thiohalospira (Sorokin et al, 2008). -, Trace amount or not detected; ND, no data available.…”

mentioning

confidence: 99%

“…The almost-complete 16S rRNA gene sequence (1476 bp) was obtained from strain XK5 T and BLAST searches revealed that the novel isolate had highest similarity to Thioalkalivibrio thiocyanodenitrificans DSM 16954 T (92.1 %; Sorokin et al 2004), followed by two other members of the same genus, Thioalkalivibrio sulfidiphilus NCCB 100376 T (91.9 %; Sorokin et al 2012) and Thioalkalivibrio denitrificans DSM 13742 T (91.8 %; Sorokin et al 2001). Except for the above three strains, other published strains showed ,91.0 % sequence similarity with strain XK5 T .…”

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confidence: 99%

Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov.

Wang

Zhao

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov. A novel Gram-stain-negative, rods or bent rods, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated XK5 T , was isolated from coastal sediment from Xiaoshi Island, Weihai, China. Optimal growth occurred at 28-35 8C (range 8-42 8C) and pH 7.0-8.0 (range pH 6.0-9.0) with 1-3 % (w/v) NaCl (range 0.5-8 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain XK5 T was 92.1 % similar to the type strain of Thioalkalivibrio thiocyanodenitrificans, 91.9 % to the type strain of Thioalkalivibrio sulfidiphilus and 91.8 % to the type strain of Thioalkalivibrio denitrificans; similarity to other species was less than 91 %. The isolate and closely related environmental clones formed a novel family level clade in the order Chromatiales. The polar lipid profile of the novel isolate consisted of phosphatidylethanolamine, phosphatidylglycerol and some other unknown phospholipids, aminolipids and lipids. Major cellular fatty acids were iso-C 17 : 1 v9c and iso-C 15 : 0 and the main respiratory lipoquinone was Q-8. The DNA G+C content of strain XK5 T was 59.3 mol%. Comparative analysis of 16S rRNA gene sequences and characterization indicated that strain XK5 T represents a novel species of a new genus within a novel family of the order Chromatiales, for which the name Woeseia oceani gen. nov., sp. nov. is proposed. The type strain of Woeseia oceani is XK5 T (5ATCC BAA-2615 T 5CICC 10905 T ). In addition, a novel family name, Woeseiaceae fam. nov., is proposed to accommodate the genus Woeseia.In the past, the taxonomy of bacteria was entirely based on simple phenotypic characteristics (Imhoff, 2005), which aroused a long-time dispute about the taxonomic position of the bacteria now classified as the Ectothiorhodospiraceae since their discovery by Pelsh (1936). Members of the Ectothiorhodospiraceae were previously treated as a genus of the Chromatiaceae. Until recently, with the application of phylogenetic taxonomy of 16S rRNA, the Ectothiorhodospiraceae was separated as a family distinct from the family Chromatiaceae (Imhoff, 1984). At the time of writing, the order Chromatiales contains five families, according to the List of Prokaryotic Names with Standing in Nomenclature (LPSN; Euzéby, 1997), and members of the order are famous for their various types of metabolism, such as photoautotrophic, photoheterotrophic, chemoautotrophic and chemoheterotrophic. Most phototrophic bacteria in the order Chromatiales can utilize reduced sulfur compounds as electron donors, which forms highly refractile globules of elemental sulfur; the family Chromatiaceae deposit the sulfur globules inside the cells while the Ectothiorhodospiraceae outside (Pfennig & Trüper, 1974). In this study, a novel chemoheterotrophic bacterium that had significant differences with the members of the Ectothiorhodospiraceae, the closest relatives of the novel isolate, is reported. Based o...

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Anaerobic growth of the haloalkaliphilic denitrifying sulfur-oxidizing bacterium Thialkalivibrio thiocyanodenitrificans sp. nov. with thiocyanate

Cited by 101 publications

References 28 publications

Conditions and Mechanisms in Thiocyanate Biodegradation

Conditions and Mechanisms in Thiocyanate Biodegradation

Dethiobacter alkaliphilus gen. nov. sp. nov., and Desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes

Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov.

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