Anaesthesia and transport of juvenile tambaquiColossoma macropomum(Cuvier, 1818) with tricaine methane-sulphonate: Implications on secondary and oxidative stress responses

Abstract

Summary The aim of this study was to evaluate the anaesthetic efficacy and the effect of sedation with tricaine (TMS®), also known as MS‐222, on secondary and oxidative stress parameters in juvenile tambaqui, Colossoma macropomum transported in hyperoxia for 2, 6 and 10 hr. Juveniles were placed in aquaria containing six different concentrations of buffered tricaine (150, 180, 210, 240, 270 and 300 mg/L) and the times for anaesthesia induction and recovery determined. Fish transported in hyperoxic conditions were investigated for glycemia, ionic concentration (K+, Ca++, Na+) and osmolality, haematocrit (Ht) and haemoglobin concentration (Hb), partial pressure of gases (pCO2 and pO2), pH and bicarbonate concentration (HCO3−) in whole blood collected from caudal vasculature. Total antioxidant capacity against peroxyl radicals (ACAP), glutathione‐S‐transferase (GST) activity and lipid peroxidation levels (TBARS) were investigated in gills, brain and liver. All concentrations of TMS® induced deep anaesthesia in juvenile tambaqui in this study. A concentration of 240 mg/L of TMS® was sufficient to induce rapid anaesthesia (<3 min) with uneventful recovery (<5 min). In light of the secondary and oxidative stress responses of fish transported using TMS®, which were generally not significantly different compared to responses of fish transported in anaesthetic‐free water, sedation with 20 mg/L is not advantageous and therefore is dispensable for the transport of this species for up to 10 hr.

The efficacy of eugenol and tricaine methanesulphonate as anaesthetics for juvenile Chinese sea bass (Lateolabrax maculatus) during simulated transport

“…As the anaesthetic concentration increased, the induction time decreased and the recovery time increased. Although the present results of the anaesthetic concentration were slightly different compared to that of other scholars (Balamurugan et al, ; Barbas et al, ; Cooke et al, ; Kiessling, Johansson, Zahl, & Samuelsen, ; Mylonas, Cardinaletti, Sigelaki, & Polzonetti‐Magni, ; Zahl, Kiessling, Samuelsen, & Hansen, ), the reason might be related to differences in the purity, species, age, and experimental environment of the anaesthetic.…”

Secondary Stress Responses in Juvenile Brazilian Flounder, Paralichthys orbignyanus, throughout and after Exposure to Sublethal Levels of Ammonia and Nitrite

“…Beyond that, monitoring of blood parameters using i‐STAT may be a useful and practical tool for a quick evaluation of stress in animals. Many recent studies have used i‐STAT‐based analyses on fish blood (Barbas et al , ; Hvas et al ; Calabrese et al ; Pereira‐Cardona et al ).…”

The anaesthetic efficacy of Eucalyptus globulus essential oil on silver catfish ( Rhamdia quelen )

“…Therefore, essential oils (EO) are an excellent alternative to replace synthetic products. For this reason, they have recently been widely tested as an anaesthetic for fish (Barbas et al, 2017;Boijink et al, 2016;da Silva et al, 2019;Hajek, 2011;Parodi et al, 2014;Ribeiro et al, 2016;Silva et al, 2012;Weber et al, 2009;Zeppenfeld et al, 2019).…”

“…Serum levels of cortisol, glucose and lactate have been used as indicators of stress in fish subjected to anaesthesia (Barbas et al, 2017;Boijink et al, 2016;Corso et al, 2019;Cunha et al, 2010;Ribeiro et al, 2016).…”

“…At the chronic stress level, we have tertiary response characterized by immunosuppression, negatively affecting reproduction, metabolism and development (Barton & Iwama, 1991). Serum levels of cortisol, glucose and lactate have been used as indicators of stress in fish subjected to anaesthesia (Barbas et al, 2017; Boijink et al, 2016; Corso et al, 2019; Cunha et al, 2010; Ribeiro et al, 2016).…”