Analgesia, anesthesia, and euthanasia of aquatic animals

Gressler, Luciane Tourem; Heinzmann, Berta Maria; Baldisserotto, Bernardo

doi:10.1016/b978-0-12-821339-1.00007-6

Cited by 5 publications

(5 citation statements)

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“…30 In this respect, insufficient branchial ventilation is a well-documented undesirable side effect of euthanasia. 31 Furthermore, Dong et al 32 reported that exposure to MS-222 and eugenol…”

Section: Discussionmentioning

confidence: 99%

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Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

et al. 2023

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In experimental procedures inevitably leading to the sacrifice of animals, suitable measures should be taken to minimise their pain and suffering as much as possible, as well as to prevent any modification or masking in the experimental results obtained. An overdose of anaesthetic is the method of euthanasia most employed in fish, since it is effective and easy to apply. Our objectives were to compare the efficacy of eugenol and of tricaine methanesulfonate (MS-222) as euthanasia agents in zebrafish, and to make a histological evaluation of the possible effects derived from their application. The concentrations established for eugenol were 0.25 and 0.35 mg/mL, and those for MS-222 were 0.25 and 0.50 mg/mL, for both the buffered solution and the non-buffered one. Eugenol turned out to be a stronger euthanasia agent than MS-222 in zebrafish, presenting with significantly shorter euthanasia times. However, the exposure of the fish to euthanasia doses of eugenol triggered branchial alterations, in addition to serious lesions and changes in their nerve tissue. The results obtained with MS-222 also revealed a marked branchial alteration derived from its use. In this respect, the addition of a buffer to the MS-222 solution enhanced the effectiveness of the drug, with significantly shorter euthanasia times being achieved than with the non-buffered solution, and diminished the severity of the lesions described. We therefore determined that the buffered MS-222 solution is the most effective, reliable and safest method of euthanasia for use in research on zebrafish.

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Section: Discussionmentioning

confidence: 99%

“…30 In this respect, insufficient branchial ventilation is a well-documented undesirable side effect of euthanasia. 31 Furthermore, Dong et al. 32 reported that exposure to MS-222 and eugenol regulated the metabolic pathways and triggered an immunoreaction in the gills of Asian sea bass ( Lateolabrax maculatus ).…”

Section: Discussionmentioning

confidence: 99%

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

et al. 2023

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“…While it is uncertain whether they are appropriate or not, it is preferable to grant the benefit of the doubt than to cause discomfort or pain to the animal. For example, hypothermia does not provide analgesia, but only immobilization and torpor, that is, even if the animal is immobile, sensation and responses to painful stimuli are still present ( Birch et al., 2021 ; Gressler et al., 2021 ; Pellet et al., 2020 ). Hypothermia should never be used or be acceptable for any painful or invasive procedure ( Cooper, 2004 ).…”

Section: Anaesthesiamentioning

confidence: 99%

Anaesthesia of decapod crustaceans

Valente

2022

Veterinary and Animal Science

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“…A literatura não há muitos artigos relatando sobre os efeitos tóxicos em humanos. Contudo, devido a este componente ser utilizado em larga escala como anestésico em animais (Gressler, Heinzmann, Baldisserotto, 2021), a maior parte de estudos envolvendo sobre sua toxicidade é relatada em peixes ou animais aquáticos.…”

Section: Efeitos Tóxicos Do Eugenolunclassified

Estudos In silico sobre as atividades anticancerígenas do Eugenol presente no Cravo Da Índia (Syzygium aromaticum)

Oliveira

Arruda

2021

RSD

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Objetivo: Investigar as propriedades anticancerígenas do eugenol usando métodos In silico. Metodologia: é um artigo original utilizando a metodologia In silico atraves dos softwares PASS (Prediction of activity spectra for substances) online e GUSAR disponíveis de forma gratuita e livre. Resultados: Os resultados do sistema operacional online PASS demonstraram diversas atividades biológicas positiva, tais quais efeito antimutagênico, estimulante de caspase, tratamento pré neoplásico e agonista de apoptose tendo uma probabilidade de atividade (PA) bem superior a 0,700. Tais achados reforçam aos descritos na literatura que evidenciam um potencial antineoplásico devido o componente ter efeitos antioxidante, e estar vinculado a expressão de genes envolvidos na apoptose e inflamação. Tal como efeitos na regulação negativa de Bcl-2, COX-2 e IL-1ß, e ações na regulação positiva de P53, Bax e expressão ativa de Caspase-3, caspase-9 além de induzir a via da apoptose mitocondrial. Já pelo software GUSAR, foi observado a toxicidade, demonstrando que na categoria 4, estão as vias de administração intravenosa, oral, e subcutânea, indicando que é pouco tóxico. Já na categoria 5 enquadra-se a via intraperitoneal é relativamente atóxico. Tal achado ainda é obscuro e possui poucos artigos científicos para o embasamento. Conclusão: O Eugenol possui efeitos favoráveis para o tratamento e prevenção de diversos canceres e células mutagênicas, porém ainda há pouco conhecimento sobre a toxicidade deste componente. Assim, necessita de um maior aprofundamento sobre a temática.

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Analgesia, anesthesia, and euthanasia of aquatic animals

Cited by 5 publications

References 293 publications

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

Anaesthesia of decapod crustaceans

Estudos In silico sobre as atividades anticancerígenas do Eugenol presente no Cravo Da Índia (Syzygium aromaticum)

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