Analgesic effects of mad honey (grayanotoxin) in mice models of acute pain and painful diabetic neuropathy

Gunduz, Abdülkadir; Eraydin, Ismet; Turkmen, Süha; Kalkan, Ömer Faruk; Türedi, Süleyman; Eryiğit, Umut; Ayar, Ahmet

doi:10.1177/0960327113482693

Cited by 20 publications

(16 citation statements)

References 21 publications

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“…No previous studies on chemical metabolizing enzyme and genotoxic effects of mad honey or GTX have been found. The aim of the study is to determine the potential adverse effects that can occur in tissues and organs at different dose intervals in single and repeated exposures of honey, which are frequently consumed for different purposes 63,64 Mad honey can create very different and independent effects on the biological system. This was investigated to determine the possible effects on liver and kidney tissue, which is at the forefront of metabolism and elimination.…”

Section: Introductionmentioning

confidence: 99%

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

et al. 2017

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A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg body weight (b.w.); acute stage), at a dose of 7.5 g kg b.w. for 21 days (subacute stage), and at a dose of 5 g kg b.w. for 60 days (chronic stage). Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined. Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results. Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.

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Section: Introductionmentioning

confidence: 99%

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

et al. 2017

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“…The fi eld where it is most widely used as a natural support product is to enhance sexual performance (4). Previous experimental studies have shown that GTX possesses analgesic, blood pressure lowering and heart rate lowering effects (5,6) The great majority of affected individuals in published case series and studies of mad honey intoxications consist of adult males aged 40 or over (3,4,8,9). The reason for this is probably the widespread belief that mad honey enhances male sexual performance (4).…”

Section: Introductionmentioning

confidence: 99%

The effect of mad honey on testosterone levels of male rats

Tatlı¹,

Karaca²,

Turkmen³

et al. 2017

BLL

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OBJECTIVE: We aimed to investigate the effect of mad honey on sexual performance. BACKGROUND: In traditional medicine in Turkey, mad honey is used to improve appetite, to heighten mental alertness, to reduce joint pain, to eliminate gastrointestinal system pains and to increase sexual performance. METHODS: In this experimental animal study eighteen Sprague Dawley male rats were randomized into three groups, a control group, a normal honey group and a mad honey group. Rats in the treatment groups were given a daily dose of 80 mg/kg normal honey or mad honey throughout the 30-day study period. Total testosterone, free testosterone, FSH, LH, estradiol, and progesterone levels were subsequently investigated from blood sera on day 30. RESULTS: Comparison of blood total testosterone levels among the groups revealed signifi cantly higher levels in the mad honey group compared to the normal honey and control groups (p = 0.006, p = 0.00). Free testosterone levels were also signifi cantly higher in the mad honey group than in the normal honey and control groups (p = 0.023, p = 0.01). No statistically signifi cant differences were determined for other hormonal measurements. CONCLUSIONS: This study revealed a signifi cant increase in both total and free testosterone levels in mad--honey group (Tab. 1, Fig. 2

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“…by a single dose. 6,16,17 Groups on the chronic effects. Each group included 10 animals, and GTX-III dosages were administered i.p.…”

Section: Formation Of the Experiments Groupsmentioning

confidence: 99%

The cardiotoxic effects of acute and chronic grayanotoxin-III in rats

2019

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The purpose of this study is to histologically and immunohistochemically determine the changes created by grayanotoxin-III (GTX-III), which is a sodium channel neurotoxin, on heart tissues in different dosages. Rats were randomly divided into 10 groups to determine the acute and chronic effects of GTX-III. While the rats in groups 1 and 6 were control rats, the rats in groups 2–5 (1, 2, 4, and 8 μg/kg bw GTX-III) received a single dose of intraperitoneal GTX-III, and the rats in groups 7–10 received GTX-III every day for 3 weeks. As a result of the trial, in the heart tissues, histopathological changes were determined by hematoxylin–eosin staining, interleukin-1 (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and brain natriuretic peptide (BNP) were determined by the avidin–biotin peroxidase method, and apoptosis was examined by immunohistochemistry (IHC) analysis and the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining method. In the immunohistochemistry sense, while the BNP level in the AGTX-III groups did not vary significantly, an increase in dosage significantly increased the IL-6, IL-1β, and TNF-α levels in comparison to the control groups. In their comparison to the control groups, the BNP levels increase and the IL-6 and IL-1β levels decreased in the CGTX-III groups. TUNEL analysis revealed that apoptosis increased in both the acute and chronic groups.

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Analgesic effects of mad honey (grayanotoxin) in mice models of acute pain and painful diabetic neuropathy

Cited by 20 publications

References 21 publications

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

The effect of mad honey on testosterone levels of male rats

The cardiotoxic effects of acute and chronic grayanotoxin-III in rats

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