Análise da diversidade genética entre acessos de banco ativo de germoplasma de algodão

Carvalho, Luiz Paulo de; Lanza, Marcelo Abreu; Fallieri, Joel; Santos, J. W. dos

doi:10.1590/s0100-204x2003001000003

Cited by 30 publications

(29 citation statements)

References 6 publications

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“…The experimental area was homogeneous and each block consisted of a single 5 m row, with 0.5 m spacing between rows. This design was adopted owing to the small number of available seeds and according to the methodology used by germplasm banks, where the genotypes are planted in single rows, without repetitions (Carvalho et al, 2003;Chiorato et al, 2007). The agronomic traits were evaluated using the mean data of six plants collected at random within the block.…”

Section: Analysis Of Phenotypic Datamentioning

confidence: 99%

Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers

Villela¹,

Uneda-Trevisoli²,

Silva³

et al. 2014

Afr. J. Biotechnol.

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The aim of this study was to estimate the genetic diversity in 74 RR soybean cultivars from different Brazilian breeding programs. Analyzes were based on multivariate statistical techniques from phenotypic characteristics and microsatellite molecular markers (SSR). Ten agronomic traits were used in the analysis of the Euclidean distance, Tocher's clustering, UPGMA clustering and principal component analysis. Eighty-six of 100 SSR primer-pairs studied were selected based on their polymorphism information content, and analyzed using Jaccard Coefficient and UPGMA clustering method. The cultivars were clustered into seven groups according to the UPGMA and Tocher's methods, based on agronomic traits, while molecular analysis identified six groups. The phenotypic distances ranged from 0.46 to 9.79 and the dissimilarity measurements, based on SSR molecular markers, ranged from 0.07 to 0.73. Both results from agronomic traits and molecular markers showed that there is genetic variability among the RR cultivars and that the Monsanto breeding program has the most divergent germplasm. The analyzed agronomic traits and the chosen SSR markers were effective in assessing the genetic diversity among genotypes, besides proving to be useful for characterizing genetic variability of soybean germplasm.

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Section: Analysis Of Phenotypic Datamentioning

confidence: 99%

Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers

Villela¹,

Uneda-Trevisoli²,

Silva³

et al. 2014

Afr. J. Biotechnol.

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“…A partição da variação entre populações naturais de outras espécies tropicais, perenes e alógamas, analisadas também por marcadores RAPD, tem apresentado porcentagem próxima ou mais elevada que a encontrada neste estudo (Araújo, 2001;Carvalho et al, 2003;Fontaine et al, 2004;Wadt & Kageyama, 2004;Zucchi et al, 2003). Para Zucchi et al (2003), o alto nível de diferenciação genética entre populações de cagaiteira (φ ST =0,2703) é decorrente de efeitos da deriva genética, ocasionados pela ação antrópica e pela predominância de fluxo gênico a pequenas distâncias.…”

Section: Resultsunclassified

Diferenciação genética entre procedências de açaizeiro por marcadores RAPD e SSR

Oliveira

Damasceno‐Silva

2008

Rev. Bras. Frutic.

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RESUMO -Diferenciação genética refere-se à distribuição da variabilidade entre e dentro de populações, procedências ou outros tipos de agrupamentos. Seu conhecimento é importante para estabelecer estratégias de coleta, conservação e manejo de germoplasma de qualquer espécie. Neste trabalho, avaliou-se a diferenciação genética entre procedências de açaizeiro que compõem a coleção da Embrapa Amazônia Oriental, por meio de marcadores RAPD e SSR. Para tanto, foram utilizados DNAs de 107 acessos, representantes de 17 regiões geográficas diferentes e utilizados em PCR com 28 primers RAPD e sete primers SSR. Os dados foram submetidos à análise de variância molecular (AMOVA) com estrutura hierárquica desbalanceada. Altos níveis de diferenciação genética foram registrados entre procedências, com 0,301 para o marcador dominante e 0,242 para o co-dominante. Para os dois marcadores, a AMOVA apresentou grande variabilidade dentro das procedências (acima de 69%). O pequeno tamanho amostral das procedências do Maranhão pode ter contribuído para a diferenciação significativa entre procedências. Os dados obtidos por esses marcadores foram concordantes quanto à distribuição da variação genética entre e dentro de procedências dessa palmeira. Termos para indexação: Amazônia, germoplasma, Euterpe, palmeira,variação genética. GENETIC DIFFERENTIATION IN PROVENANCES OF AÇAÍ TREE BY RAPD AND SSR MARKERSABSTRACT -Genetic differentiation refers to the distribution of the variability among and within populations, provenances or other grouping types. This knowledge is important to establish collection strategies, conservation and germplasm management of any species. In this work, the genetic differentiation was evaluated among provenances of açaí tree from Embrapa Eastern Amazon germplasm collection using RAPD and SSR markers. DNAs of 107 accessions, representing 17 different geographic regions were used in PCR reactions with 28 RAPD primers and seven SSR primers. The obtained data was submitted to analyses of molecular variance (AMOVA) with unbalanced hierarchical structure. High levels of genetic differentiation were registered among provenances, with 0.301 for the dominant marker (RAPD) and 0.242 for the codominante (SSR). For the two markers, AMOVA showed a great variability within the provenances (above 69%). The small sampling size of Maranhão provenances might have contributed to the significant differentiation among these provenances. The data obtained by these markers were in agreement with in what concerns the distribution of the genetic variation among and within the provenances of this palm tree. Index terms: Amazon, Germplasm, Euterpe, Palm, Genetic variation. INTRODUÇÃOA diferenciação genética refere-se à distribuição da variabilidade entre e dentro de populações e subpopulações naturais, procedências ou outros tipos de agrupamentos, onde os alelos e os genótipos ocorrem de maneira heterogênea no espaço e no tempo, isto é, resultado da ação de forças evolutivas que atuam dentro do contexto de cada espécie (Hamrick, 1982). Ess...

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“…Most cotton breeding programs in Brazil make use of conventional crossing methods, employing the natural variation of genotypes to transfer desirable phenotypic traits to the progeny (CARVALHO et al, 2003). However, depending on the type of gene action, such procedures can reduce or inhibit the expression of other important characteristics in the parental strains (strength, colouration, length, chemical compatibility, water absorption, thermal properties and resistance), reducing the expectation of the development of promising cultivars (NOVAES et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of gene sequences expressed in cotton fiber

Coutinho

Guimarães

Vidal

2016

Revista Ciência Agronômica

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-Cotton fiber are tubular cells which develop from the differentiation of ovule epidermis. In addition to being one of the most important natural fiber of the textile group, cotton fiber afford an excellent experimental system for studying the cell wall. The aim of this work was to isolate and characterise the genes expressed in cotton fiber (Gossypium hirsutum L.) to be used in future work in cotton breeding. Fiber of the cotton cultivar CNPA ITA 90 II were used to extract RNA for the subsequent generation of a cDNA library. Seventeen sequences were obtained, of which 14 were already described in the NCBI database (National Centre for Biotechnology Information), such as those encoding the lipid transfer proteins (LTPs) and arabinogalactans (AGP). However, other cDNAs such as the B05 clone, which displays homology with the glycosyltransferases, have still not been described for this crop. Nevertheless, results showed that several clones obtained in this study are associated with cell wall proteins, wall-modifying enzymes and lipid transfer proteins directly involved in fiber development.Key words: Gossypium hirsutum L.. cDNA Library. Transfer proteins. Arabinogalactan. Glycosyltransferase.RESUMO -As fibras de algodão são células tubulares que se desenvolvem a partir da diferenciação da epiderme do óvulo. Além de serem uma das mais importantes fibras naturais do grupo têxtil, as fibras do algodão fornecem um excelente sistema experimental para o estudo da parede celular. Este trabalho teve por objetivo o isolamento e a caracterização de genes expressos em fibras de algodão (Gossypium hirsutum L.) para serem empregados futuramente em trabalhos de melhoramento do algodão. Fibras da cultivar de algodão CNPA ITA 90 II foram utilizadas para extração de RNA e posterior geração de uma biblioteca de cDNAs. Foram obtidas 17 sequências, dentre estas 14 já se encontram descritas em banco de dado do NCBI (National Center for Biotechonology Information), tais como as codificadoras das proteínas transportadoras de lipídios (LTPs) e das arabinogalactana (AGP). No entanto, outros cDNAs, como o clone B05, que apresenta homologia com as glicosiltransferases, ainda não foi descrito para esta cultura. Porém, os resultados indicaram que vários clones obtidos neste trabalho estão associados a proteínas da parede celular, enzimas modificadoras de parede e proteínas de transferência de lipídeo, envolvidas diretamente no desenvolvimento da fibra. Palavras-chave:Gossypium hirsutum L.. Biblioteca de cDNA. Proteínas de transferência. Arabinogalactana. Glicosiltransferase.

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Análise da diversidade genética entre acessos de banco ativo de germoplasma de algodão

Cited by 30 publications

References 6 publications

Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers

Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers

Diferenciação genética entre procedências de açaizeiro por marcadores RAPD e SSR

Isolation and characterization of gene sequences expressed in cotton fiber

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