Analyse Des Conditions De La Fécondation in Vitro De l'ŒUF De La Lapine

Thibault, Christelle; Dauzier, L; Gérard, Micheline; Goussopoulos, Éliane

doi:10.1051/rnd:19610301

Cited by 48 publications

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“…The thinking in early investigations of fertilization in Eutheria was based, to a considerable degree, on principles established in invertebrate models. Thus, in pioneering experiments on in vitro fertilization, Thibault & Dauzier (1960) first washed rabbit oocytes to avoid supposed fertilizin/antifertilizin issues raised by invertebrate studies. In a similar vein, fissures ahead of sperm heads penetrating the rabbit, pig and sheep zona pellucida (see Dziuk & Dickman (1965)) were thought to be acrosomal filaments comparable to those in Arbacia and Saccoglossus.…”

Section: Introductionmentioning

confidence: 99%

Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Bedford

2014

Reproduction

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Therian (marsupial and eutherian) mammals have evolved a suite of novel reproductive features -seen variously in their gametes, the steps of fertilization and the male reproductive tract -whose adaptive significance remains unclear. Present evidence for the betterunderstood eutherian mammals suggests that the 'prime mover' in their evolution has been the character of the egg coat, with other such features being adaptations to the consequences of this. Its elastic thickness allows the zona pellucida to stretch to a variable degree and yet remain around the blastocyst during much or all of its expansion before implantation, but its character represents an unusual challenge for spermatozoa. Novel aspects of the acrosome related to this challenge enable it to maintain a relatively prolonged binding after the onset of the acrosome reaction, and the structure, shape and behaviour of the sperm head point to physical thrust as a major element of zona penetration -with the unique configuration of gamete fusion as a sequela of this strategy. In the male, such adaptations are reflected in sperm head formation in the testis and in sperm maturation in the epididymis involving at least the sperm head's structure, plasmalemma and acrosome. This complexity allied to a slow epididymal sperm transport, a relatively modest sperm production and the brief life span of mature spermatozoa kept above the cauda epididymidis could account for the evolution of the sperm storage function -a development seemingly linked, in turn, to the need for sperm capacitation and scrotal evolution.Reproduction (2014) 147 R43-R52

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Section: Introductionmentioning

confidence: 99%

Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Bedford

2014

Reproduction

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“…Attempts to fertilize mammalian eggs in vitro go back to 1870s when Schenk [42] first reported cleavage of guinea pig eggs after in vitro insemination using epididymal spermatozoa. Although Pincus and Enzmann [43] and Venge [44] reported the birth of IVFgenerated rabbit pups, it was not until 1954 when Thibault et al [45] and Chang [46] presented unequivocal evidence of successful IVF, again using the rabbit. The use of "capacitated" spermatozoa collected from the uterus of mated females was the key to their success (for early history of IVF, see Austin [2]; Chang [47]).…”

Section: In Vitro Fertilization (Ivf): History and Todaymentioning

confidence: 99%

“…Because invertebrate animals such as sea urchins and starfish produce millions of mature eggs at one time and their fertilization and embryo development can be followed readily in a dish of seawater, most of the early and important investigations of fertilization were done using gametes of aquatic invertebrate animals. In mammals, even after induction of superovulation, only a few eggs (usually up to [40][41][42][43][44][45][46][47][48][49][50] can be obtained from a single female. Furthermore, eggs are normally fertilized and develop within the female's body, which makes observations and experiments of eggs cumbersome.…”

Section: Studies Of Mammalian Fertilizationmentioning

confidence: 99%

Fertilization Studies and Assisted Fertilization in Mammals: Their Development and Future

Yanagimachi

2012

Journal of Reproduction and Development

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Abstract. Studies of mammalian fertilization progressed very slowly in the beginning because of difficulties in obtaining a large quantity of fully mature eggs at one time. With progression of techniques to collect and handle eggs and spermatozoa, research in mammalian fertilization advanced rapidly. Today, far more papers are published on mammalian gametes and fertilization than those of all other animals combined. The development of assisted fertilization and related technologies revolutionized basic research as well as human reproductive medicine and animal husbandry. Reproduction is fundamental to human and animal lives. The author lists a few subjects of his personal interest for further development of basic and applied research of gametes and fertilization. Each reader will probably have more exciting subjects of future investigation. Key words: Assisted fertilization, Egg, Fertilization, ICSI, Sperm Discovery of Mammalian Eggs, Spermatozoa and Fertilization Although it was known from ancient times that the eggs of birds and frogs were the start of new life, no one was certain whether this was true for mammals until Karl von Bare 1792-1876 (1827) discovered tiny eggs within dog and human ovaries. Interestingly, spermatozoa had been discovered 150 years earlier by Anton van Leeuwenhoek 1632-1723 (~1676)* and a medical student of Leiden, Johan Ham who saw many tiny tadpole-like creatures ("animacules") within human seminal plasma. Leewenhoek later saw same creatures in the semen of the rabbit, dog and fish. He thought that they were normal components of semen originating from the testis. Obviously, Leeuwenhoek saw spermatozoa without knowing their exact biological role. It was not until 1840s-1870s when it became clear that spermatozoa and eggs of all animals, large or small, are cells [Albert von Koelliker 1817-1905 (1840) ; Karl Gegenbaur (1861)] and that sperm entry into the egg -in particular the union of sperm and egg nuclei (sygamy) -is the essence of fertilization and provides cytological evidence of biparental inheritance [Edouard van Beneden 1846-1910 (1875) Studies of Mammalian FertilizationThe analysis of animal fertilization began from the start of the 20 th century. Because invertebrate animals such as sea urchins and starfish produce millions of mature eggs at one time and their fertilization and embryo development can be followed readily in a dish of seawater, most of the early and important investigations of fertilization were done using gametes of aquatic invertebrate animals. In mammals, even after induction of superovulation, only a few eggs (usually up to 40-50) can be obtained from a single female. Furthermore, eggs are normally fertilized and develop within the female's body, which makes observations and experiments of eggs cumbersome. Because most investigators of mammalian eggs and fertilization used fixed and stained materials, the study of mammalian fertilization progressed very slowly. It was Colin "Bunny" Austin who disclosed many details of mammalian fertilization using the...

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“…It also gave me the opportunity to inseminate them in vitro when they were mature and ready for fertilization. I was moving straight back to work done previously by Thibault, Dauzier, Austin, Chang and Yanagimachi [1][2][3][4][5][6][7][8][9].…”

Section: Working With Embryo Stem Cells and Contemplating Human Studiesmentioning

confidence: 99%

An astonishing journey into reproductive genetics since the 1950’s

Edwards¹

2005

Reprod. Nutr. Dev.

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-Training in genetics in Edinburgh in the 1950s led to a PhD on the developmental biology of mouse embryos with unusual chromosomal complements. Fundamental aspects of reproduction under study included ovulation induction, oocyte maturation and embryonic growth to blastocysts. It led to the introduction of embryo stem cells, preimplantation genetic diagnosis, the exact timing of human oocyte maturation in vitro and studies on fertilising human eggs in vitro to alleviate human infertility. My work was helped by studies on sperm capacitation and the physiology of fertilization in domestic and laboratory species by Thibault, Dauzier, Austin, Chang, Yanagimachi and others. I met Charles Thibault at a meeting in Cambridge U.K. where he criticised the work of Moricard, and then frequently on lecture circuits. Impressed by his grandeur but not his doubts about human IVF, Steptoe and I initiated human embryo transfers and the birth of Louise Brown. Details of her pregnancy had to be confidential to reduce the risks of abortion associated with the intrusion of numerous newsmen chasing the story. I was compelled to withold this information at a meeting in Paris in the late 1960s when I had to leave early to return to UK. This omission annoyed Thibault and led to our celebrated quarrel. I felt he failed to appreciate the complexity, the implications of this pregnancy and an astonishing future. So much was at stake, including IVF, stem cells and preimplantation diagnosis to help millions of patients. Some months later, our dispute was ended even if somewhat formally. Nevertheless it is a pleasure to recall how we shared so much in common. I still admire him as an inspiration to many colleagues and students, and a father figure in French agricultural research. human / embryo / reproductive genetics / stem cells / in vitro fertilizationI am honoured to be at this meeting to salute the life of Charles Thibault since I knew him over a period of 40 or 50 years. Ours was not an entirely smooth relationship, but I think we both had mutual respect for each other. I also believe we each recognized a worthy opponent or colleague when difficult times arrived. In a sense, although I never formally told him this, it was his work, and that of Chang [1], and of Austin [2], on the capacitation of spermatozoa which set me off on my travels into human IVF.

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Analyse Des Conditions De La Fécondation in Vitro De l'ŒUF De La Lapine

Abstract: SOMMAIRECe n'est qu'en ig 5 i après la découverte par CII ANG et AusTirl Ces mécanismes n'ont pu être retrouvés avec les oeufs de Brebis et de Truie.

Cited by 48 publications

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Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Fertilization Studies and Assisted Fertilization in Mammals: Their Development and Future

An astonishing journey into reproductive genetics since the 1950’s

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