Analyses of pea necrotic yellow dwarf virus-encoded proteins

Krenz, Björn; Schießl, Ingrid; Greiner, Eva; Krapp, Susanna

doi:10.1007/s11262-017-1439-x

Cited by 13 publications

(18 citation statements)

References 47 publications

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“…Importantly, this TMD-containing protein ( Table S6 ) is able to re-locate the NSP protein to the cell periphery. Thus, these results indicate that nanoviruses may utilize a system functionally similar to that of the bipartite geminiviruses, where the NSP protein acts to transfer ssDNA from nucleus to cytoplasm while the small hydrophobic MP protein transports the NSP-DNA complexes to the cell periphery for intercellular transport [204] – [206] .…”

Section: Dna-containing Plant Virusesmentioning

confidence: 89%

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Morozov

Solovyev

2020

AIMS Microbiology

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Most plant viruses code for movement proteins (MPs) targeting plasmodesmata to enable cell-to-cell and systemic spread in infected plants. Small membrane-embedded MPs have been first identified in two viral transport gene modules, triple gene block (TGB) coding for an RNA-binding helicase TGB1 and two small hydrophobic proteins TGB2 and TGB3 and double gene block (DGB) encoding two small polypeptides representing an RNA-binding protein and a membrane protein. These findings indicated that movement gene modules composed of two or more cistrons may encode the nucleic acid-binding protein and at least one membrane-bound movement protein. The same rule was revealed for small DNA-containing plant viruses, namely, viruses belonging to genus Mastrevirus (family Geminiviridae ) and the family Nanoviridae . In multi-component transport modules the nucleic acid-binding MP can be viral capsid protein(s), as in RNA-containing viruses of the families Closteroviridae and Potyviridae . However, membrane proteins are always found among MPs of these multicomponent viral transport systems. Moreover, it was found that small membrane MPs encoded by many viruses can be involved in coupling viral replication and cell-to-cell movement. Currently, the studies of evolutionary origin and functioning of small membrane MPs is regarded as an important pre-requisite for understanding of the evolution of the existing plant virus transport systems. This paper represents the first comprehensive review which describes the whole diversity of small membrane MPs and presents the current views on their role in plant virus movement.

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Section: Dna-containing Plant Virusesmentioning

confidence: 89%

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Morozov

Solovyev

2020

AIMS Microbiology

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“…SCD2 (XP_004235837) fragments were PCR amplified, first inserted into the vector pENTR-D/TOPO (Invitrogen), sequenced, and then transferred into vectors pK7FWG2.0 or pK7RWG2 [ 33 ] with L/R-Clonase TM II enzyme mix (Invitrogen). Gateway ® compatible vectors pRB- C -VenusN173 and pRB- C -VenusC155 [ 39 ] were used for bimolecular fluorescence complementation (BiFC) experiments. MP AbMV , MP AbMV aa1-180 , A.t.Pin4, and S.l.SCD2 pENTR-D/TOPO clones were recombined into these BiFC destination vectors as above.…”

Section: Methodsmentioning

confidence: 99%

Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners

Krapp

Schuy

Greiner

et al. 2017

Viruses

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Geminiviral single-stranded circular DNA genomes replicate in nuclei so that the progeny DNA has to cross both the nuclear envelope and the plasmodesmata for systemic spread within plant tissues. For intra- and intercellular transport, two proteins are required: a nuclear shuttle protein (NSP) and a movement protein (MP). New characteristics of ectopically produced Abutilon mosaic virus (AbMV) MP (MPAbMV), either authentically expressed or fused to a yellow fluorescent protein or epitope tags, respectively, were determined by localization studies in mammalian cell lines in comparison to plant cells. Wild-type MPAbMV and the distinct MPAbMV: reporter protein fusions appeared as curled threads throughout mammalian cells. Co-staining with cytoskeleton markers for actin, intermediate filaments, or microtubules identified these threads as re-organized microtubules. These were, however, not stabilized by the viral MP, as demonstrated by nocodazole treatment. The MP of a related bipartite New World begomovirus, Cleome leaf crumple virus (ClLCrV), resulted in the same intensified microtubule bundling, whereas that of a nanovirus did not. The C-terminal section of MPAbMV, i.e., the protein’s oligomerization domain, was dispensable for the effect. However, MP expression in plant cells did not affect the microtubules network. Since plant epidermal cells are quiescent whilst mammalian cells are proliferating, the replication-associated protein RepAbMV protein was then co-expressed with MPAbMV to induce cell progression into S-phase, thereby inducing distinct microtubule bundling without MP recruitment to the newly formed threads. Co-immunoprecipitation of MPAbMV in the presence of RepAbMV, followed by mass spectrometry identified potential novel MPAbMV-host interaction partners: the peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (Pin4) and stomatal cytokinesis defective 2 (SCD2) proteins. Possible roles of these putative interaction partners in the begomoviral life cycle and cytoskeletal association modes are discussed.

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“…However, the function of this viral protein is conserved, as the NSP of FBNSV could complement that of the closely related Faba bean necrotic yellows virus and the distantly related Pea necrotic yellow dwarf virus (Grigoras et al, 2018). Moreover, the mechanism may be distinct from the role of helper components in non-circulative viruses, as NSP was found to not directly bind the CP in yeast two-hybrid and bimolecular fluorescence complementation assays (Krenz et al, 2017). To date, aphid proteins involved in nanovirus transmission have not been reported.…”

Section: Virus-vector Protein Interactions Regulating Nanovirus Acquisition and Transmissionmentioning

confidence: 99%

Looking Through the Lens of 'Omics Technologies: Insights Into the Transmission of Insect Vector-borne Plant Viruses

Wilson¹,

DeBlasio²,

Alexander³

et al. 2020

Current Issues in Molecular Biology

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Insects in the orders Hemiptera and Thysanoptera transmit viruses and other pathogens associated with the most serious diseases of plants. Plant viruses transmitted by these insects target similar tissues, genes, and proteins within the insect to facilitate plant-to-plant transmission with some degree of specificity at the molecular level. 'Omics experiments are becoming increasingly important and practical for vector biologists to use towards better understanding the molecular mechanisms and biochemistry underlying transmission of these insect-borne diseases. These discoveries are being used to develop novel means to obstruct virus transmission into and between plants. In this chapter, we summarize 'omics technologies commonly applied in vector biology and the important discoveries that have been made using these methods, including virus and insect proteins involved in transmission, as well as the tri-trophic interactions involved in host and vector manipulation. Finally, we critically examine the limitations and new horizons in this area of research, including the role of endosymbionts and insect viruses in virus-vector interactions, and the development of novel control strategies. Advances in whole genome sequencing of insect vectors of plant viruses fuels discoveryMajor advances in next generation sequencing technologies and their increased affordability has led to an explosion in the availability of whole-genome sequence data for each biological player in the virus-vector-host relationship: virus, vector and plant (Fig. 6.1A). To date, draft reference genome sequences have been completed and/or published for nearly all the major types of agricultural vectors including whiteflies (Chen et al., 2016), planthoppers (Zhu et al., 2017), aphids (Consortium, 2010Wenger et al., 2017), and psyllids (Saha et al., 2017). Whole genome studies have shed light on the gene families in these insects that are critical for plant adaptation, insecticide resistance, and virus transmission (

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Analyses of pea necrotic yellow dwarf virus-encoded proteins

Cited by 13 publications

References 47 publications

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners

Looking Through the Lens of 'Omics Technologies: Insights Into the Transmission of Insect Vector-borne Plant Viruses

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