Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in <i>Nicotiana plumbaginifolia</i> Cells

Lecourieux, David; Mazars, Christian; Pauly, Nicolas; Ranjeva, Raoul; Pugin, Alain

doi:10.1105/tpc.005579

Cited by 352 publications

(338 citation statements)

References 98 publications

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“…Indeed, CBEL triggered a bimodal increase in [Ca 2þ ] cyt when administered to aequorin-transformed BY-2 tobacco cells and not to tobacco protoplasts. Induction of biphasic increases of [Ca 2þ ] cyt have been reported for tobacco cells treated with cryptogein and oligouronide elicitors (Lecourieux et al, 2002) and Pep-13-treated parsley (Petroselinum crispum) cells (Blume et al, 2000). In these studies, the induced calcium fluxes were shown to be tightly correlated to elicitor perception and were required for defense activation.…”

Section: Discussionmentioning

confidence: 79%

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

et al. 2006

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The cellulose binding elicitor lectin (CBEL) from Phytophthora parasitica nicotianae contains two cellulose binding domains (CBDs) belonging to the Carbohydrate Binding Module1 family, which is found almost exclusively in fungi. The mechanism by which CBEL is perceived by the host plant remains unknown. The role of CBDs in eliciting activity was investigated using modified versions of the protein produced in Escherichia coli or synthesized in planta through the potato virus X expression system. Recombinant CBEL produced by E. coli elicited necrotic lesions and defense gene expression when injected into tobacco (Nicotiana tabacum) leaves. CBEL production in planta induced necrosis. Site-directed mutagenesis on aromatic amino acid residues located within the CBDs as well as leaf infiltration assays using mutated and truncated recombinant proteins confirmed the importance of intact CBDs to induce defense responses. Tobacco and Arabidopsis thaliana leaf infiltration assays using synthetic peptides showed that the CBDs of CBEL are essential and sufficient to stimulate defense responses. Moreover, CBEL elicits a transient variation of cytosolic calcium levels in tobacco cells but not in protoplasts. These results define CBDs as a novel class of molecular patterns in oomycetes that are targeted by the innate immune system of plants and might act through interaction with the cell wall.

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Section: Discussionmentioning

confidence: 79%

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

et al. 2006

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“…Plant GLRs were confirmed recently to be NSCC (Price et al, 2012;Forde, 2014). Therefore, activated by Glu, GLR may mediate stress-induced Ca 2+ uptake, with a consequent rise in cytosolic Ca 2+ , that in turn can activate the plasma membrane NADPH oxidase (Lecourieux et al, 2002), leading to elevated H 2 O 2 levels and a consequent formation of OH $ (as discussed above), leading to a massive K + efflux via OH $ -activated outward-rectified (GORK) K + channels (Demidchik et al, 2010). Thus, the strong increase in Glu levels found in the root apex (Table I) may be an additional factor exacerbating a stress-induced decrease in the cytosolic K + pool in this zone, leading to the activation of caspase-like enzymes and, finally, programmed cell death (Fig.…”

Section: Changes In Primary Metabolism Exacerbate Differential K + Rementioning

confidence: 94%

Cell-Type-Specific H⁺-ATPase Activity in Root Tissues Enables K⁺ Retention and Mediates Acclimation of Barley (Hordeum vulgare) to Salinity Stress

et al. 2016

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While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue-and treatment-specific manner. Although salinity-induced transient net Na + uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na + , suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na + exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K + efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K + retention ability are (1) an intrinsically lower H + -ATPase activity in the root apex, (2) greater saltinduced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2-to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.

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“…Ca 2+ has been proposed as important signaling molecule playing role as an intracellular secondary messenger in plants under various abiotic stresses, but few studies have explored its importance under biotic stress also (Blume et al 2000;Lecourieux et al 2002). Cyclic nucleotide-gated channels (CNGCs)-Ca 2+ influx has been studied in Arabidopsis, which showed that this influx is mediated by CNGC 2, CNGC 4, CNGC 11 and CNGC 12 after elicitor perception (Urquhart et al 2011).…”

Section: Calcium Signalingmentioning

confidence: 99%

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Swarupa

Ravishankar

Rekha

2014

Planta

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Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells

Abstract: Cell suspensions obtained from

Cited by 352 publications

References 98 publications

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

Cell-Type-Specific H⁺-ATPase Activity in Root Tissues Enables K⁺ Retention and Mediates Acclimation of Barley (Hordeum vulgare) to Salinity Stress

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

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Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells

Abstract: Cell suspensions obtained from

Cited by 352 publications

References 98 publications

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

Cell-Type-Specific H+-ATPase Activity in Root Tissues Enables K+ Retention and Mediates Acclimation of Barley (Hordeum vulgare) to Salinity Stress

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

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Cell-Type-Specific H⁺-ATPase Activity in Root Tissues Enables K⁺ Retention and Mediates Acclimation of Barley (Hordeum vulgare) to Salinity Stress