Analysis and Monitoring of Oxidative Stress in Exercise and Training by FTIR Spectrometry

Petibois, Cyril; Déléris, Gérard

doi:10.1123/ijspp.3.2.119

Cited by 15 publications

(12 citation statements)

References 43 publications

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“…A detailed analysis of the spectral range below 1300 cm −1 indicates several specific changes of bands intensities assigned mainly to the C─O vibrations of saccharides (eg, glucose, lactate, glycerol) and RNA . As shown in Figures C and III, we can distinguish three characteristic trends of alterations of the bands intensities associated probably with different metabolic pathways of sugars and nucleic acids activated in the distinctive phases of cancer development.…”

Section: Resultsmentioning

confidence: 80%

“… Owen and co‐workers already showed the importance of the increased absorbance of this band in recognition of ovarian cancer in plasma of patients. In our study, we observe an increase of this absorbance in the macrometastatic phase (week 4), whereas the Spearman coefficient calculated for all phases of the tumor growth is high (ca. 0.76) and statistically significant on contrary to metastasis development, see Figure IIIA and Table .…”

Section: Resultsmentioning

confidence: 98%

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Fourier transform infrared spectroscopic signature of blood plasma in the progression of breast cancer with simultaneous metastasis to lungs

Blat

Wiercigroch

Smęda

et al. 2019

Journal of Biophotonics

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Despite advanced diagnostic techniques used for detecting cancer, this disease still remains a leading cause of death in the developed world. What is more, the greatest danger for patients is not related with growing of tumor but rather with metastasis of cancer cells to the distant organs. In this study, Fourier transform infrared (FTIR) spectroscopy was used to track chemical changes in blood plasma to find spectral markers of metastatic breast cancer during the disease progression. Plasma samples were taken 1‐5 weeks after orthotropic inoculation of 4T1 metastatic breast cancer cells to mice. The earliest changes detected by FTIR spectroscopy in plasma were correlated with unsaturation of phospholipids and secondary structures of proteins that appeared 2 and 3 weeks, respectively, after 4T1 cells inoculation (micrometastatic phase). Significant alternations in the content and structure of lipids and carbohydrates were identified in plasma at the later stages (macrometastatic phase). When large primary tumors in breast and macrometastases in lung were developed, all bands in FTIR spectra significantly differed from those at earlier phases of the cancer progression. In conclusion, we showed that each phase of the breast cancer progression and its pulmonary metastasis can be characterized by a specific panel of spectral markers.

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Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 98%

Fourier transform infrared spectroscopic signature of blood plasma in the progression of breast cancer with simultaneous metastasis to lungs

Blat

Wiercigroch

Smęda

et al. 2019

Journal of Biophotonics

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“…It has been directly shown that free radicals flow out from an active muscle bed in an intensity-dependent manner (Bailey et al, 2004). There are also some data to indicate that erythrocytes and plasma molecules are influenced by exercise-induced oxidative stress (Petibois & Déléris, 2008).…”

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confidence: 99%

Effects of Oral Supplementation With Plant Superoxide Dismutase Extract on Selected Redox Parameters and an Inflammatory Marker in a 2,000-m Rowing-Ergometer Test

Skarpańska-Stejnborn¹,

Basta²,

Deskur-Śmielecka³

et al. 2011

International Journal of Sport Nutrition and Exercise Metabolism

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The aim of this study was to investigate the effect of plant superoxide dismutase extract (GliSODin) supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. The double-blinded study included 19 members of the Polish rowing team who were participating in a preparatory camp. Subjects were randomly assigned to the supplemented group (n = 10), who received 2 capsules (500 mg) of GliSODin extract once daily for 6 weeks, or the placebo group (n = 9). At the beginning and end of the study, subjects performed a 2,000-m maximum-effort test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase (SOD) activity, glutathione peroxidase activity, and concentrations of thiobarbituric-acid-reactive substances. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and C-reactive protein and lactate dehydrogenase concentrations were measured in serum. After supplementation, SOD activity was significantly higher (p = .0037) in the supplemented group than the placebo group, and C-reactive protein was significantly (p = .00001) lower in athletes receiving GliSODin than those in the placebo group. In conclusion, supplementation with an extract rich in SOD activity promoted antioxidant status and protected against increased inflammation in the serum of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

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“…Thus, the aim of the study was to compare the erythrocyte PLs in T2DM to those of healthy subjects after ingestion of a high-fat meal. In the present study, Fourier Transform Infrared spectroscopy (FTIR) was used to rapidly screen any changes in C-H stretching vibrations that exclusively arise from PLs in the erythrocyte [13]. Phosphatidylcholine (PC) is the predominant molecular species in erythrocyte PLs and is primarily involved in the PL remodelling process [14].…”

Section: Introductionmentioning

confidence: 99%

The Alterations of Erythrocyte Phospholipids in Type 2 Diabetes Observed after Oral High-Fat Meal Loading: The FTIR Spectroscopic and Mass Spectrometric Studies

Sirikwanpong

Dahlan

Ngamukote

et al. 2010

J. Clin. Biochem. Nutr.

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Little is known about the postprandial remodelling of erythrocytes phospholipids (PLs) in type 2 diabetics (T2DM). Therefore, this study aims to compare the alterations of erythrocyte PLs in T2DM to those of healthy subjects after ingestion of a high-fat meal. Eleven T2DM and ten healthy subjects underwent a high-fat meal loading. Erythrocytes were isolated from blood obtained after fasting and 4 h after the meal. Fourier Transform Infrared (FTIR) spectroscopy was initially used to screen erythrocyte PLs by monitoring C-H stretching vibrations. Phosphatidylcholine (PC) molecular species were further investigated by Liquid Chromatography-Electrospray Ionisation-Mass Spectrometry (LC-ESI-MS). For the control group, FTIR revealed postprandial changes in C-H stretching vibrations, particularly of the olefinic band. These findings were supported by LC-ESI-MS data, showing marked changes in PC molecular species, especially of the PC34:1 (where 34 and 1 mean the summed number of carbons and double bonds, respectively). However, similar changes of those were not apparent in the T2DM group. Our results reveal marked postprandial alterations of erythrocyte PC species in healthy subjects whereas only mild alterations are observed in T2DM. The discrepant effects of high-fat meal loading suggest abnormal PC remodelling in the diabetic erythrocyte that may affect its membrane fluidity and integrity.

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Analysis and Monitoring of Oxidative Stress in Exercise and Training by FTIR Spectrometry

Cited by 15 publications

References 43 publications

Fourier transform infrared spectroscopic signature of blood plasma in the progression of breast cancer with simultaneous metastasis to lungs

Fourier transform infrared spectroscopic signature of blood plasma in the progression of breast cancer with simultaneous metastasis to lungs

Effects of Oral Supplementation With Plant Superoxide Dismutase Extract on Selected Redox Parameters and an Inflammatory Marker in a 2,000-m Rowing-Ergometer Test

The Alterations of Erythrocyte Phospholipids in Type 2 Diabetes Observed after Oral High-Fat Meal Loading: The FTIR Spectroscopic and Mass Spectrometric Studies

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