Analysis Distribution of ³²P Radioisotope in Silicone Patch Using Autoradiography Scanner

Abstract

Silicone patch has active ingredient of radioisotope Phosphorus-32 (32P) in the form of chromic phosphate (Cr32PO4). Radioisotope 32P is a β− (beta) emitter radionuclide with an energy of 1.71 MeV, having a half-life (T½) 14.3 days. A silicone patch with radioactive content of the radioisotope 32P has been used for keloid therapy. Radioisotope 32P as an active substance releases beta emitter continuously which causes the death of fibroblast and inhibits cell proliferation from keloid. In order to provide more optimal results, the distribution of chromic phosphate in silicone patches should be spread evenly. In this case, it provides a good therapeutic effect because of the energy of beta emitter is being released uniform. A chromic phosphate can be made from chromic acid reduction (redox) and phosphoric acid-containing radioisotope 32P by reducing Chrom VI to Chrom III using sodium sulfite (Na2SO3) reducing agent. In this study, we will determine the distribution of radioisotope 32P in silicone patches using an autoradiography scanner. Data from enumeration results were calculated statistically to obtain a relative standard deviation (RSD). The result shows that silicone patch sample has RSD of 0.036% with an average value of 14009482.6 ± 5041.4DLU (digital light unit) for lane and column size (10 x 14).