Analysis for Apoptosis and Necrosis on Adipocytes, Stromal Vascular Fraction, and Adipose-Derived Stem Cells in Human Lipoaspirates after Liposuction

Wang, Wei Z.; Fang, Xiaojie; Williams, Shelley; Stephenson, Linda L.; Baynosa, Richard C.; Wong, Nancy; Khiabani, Kayvan T.; Zamboni, William A.

doi:10.1097/prs.0b013e3182729ff7

Cited by 29 publications

(20 citation statements)

References 33 publications

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“…ASC viability is dependent on cell proliferation, which also was significantly reduced by Octenisept®. In contrast to apoptosis, which is a well-controlled form of cell death that may be inhibited by certain measures, Octenisept® induces necrosis, an irreversible and therefore more detrimental form of cell death (28, 29). Necrosis is followed by an inflammatory response, which may further perpetuate the critical condition of wound repair disorders (30).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Antiseptics on Adipose-Derived Stem Cells

Kim

Ott

Boecker

et al. 2017

Plastic &Amp; Reconstructive Surgery

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Background Although chemical antiseptics are the most basic measure to control wound infection and frequently come into contact with subcutaneous adipose tissue, no studies have evaluated their toxicity on adipose tissue and its cell fractions. In the present study, the effects of the following antiseptics on adipose-derived stem cells (ASC) were evaluated: Betaisodona® (povidone iodine), Lavasept®/Prontosan® (polyhexanide), Mafenide acetate, and Octenisept® (octenidinedihydrochloride). Methods Human ASCs were harvested from healthy donors. ASC viability was measured after treatment with different concentrations of antiseptics over five days. Furthermore, the effect on the proliferation, adipogenic differentiation, and apoptosis/necrosis of ASCs was analyzed. Finally, the mRNA expression of the stem cell markers CD29, CD34, CD73, CD90, and CD105 was detected. Results Octenisept® and Betaisodona® significantly reduced ASC proliferation and differentiation and led to considerable ASC necrosis. Octenisept® decreased ASC viability at the lowest concentrations tested and all stem cell markers were down-regulated by Octeniseptr® and Betaisodona®. Lavasept® and Prontosan® both led to reduced ASC viability, proliferation, differentiation, and increased apoptosis/necrosis, though the effects were less pronounced than those of Octenisept® and Betaisodona®. ASCs survived treatment with Mafenide acetate even at high concentrations, and Mafenide acetate showed minimal negative effects on their proliferation, adipogenic differentiation, cell death, and stem cell marker expression. Conclusion Mafenide acetate may be regarded as a feasible antiseptic for the treatment of wounds with exposed adipose tissue due to its low ASC toxicity. While Lavasept® and Prontosan® are possible alternatives, Octenisept® and Betaisodona® may only be used in highly diluted solutions.

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Section: Discussionmentioning

confidence: 99%

The Effect of Antiseptics on Adipose-Derived Stem Cells

Kim

Ott

Boecker

et al. 2017

Plastic &Amp; Reconstructive Surgery

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“…However, despite promising clinical potential, a detailed understanding of the putative molecular mechanisms for ADSC-mediated reversal of RTX-injury remains elusive ( 36 , 44 , 57 ). Additionally, concerns have been raised that fat grafting following cancer treatment may enhance tumorigenesis in a former cancer bed ( 58 – 60 ). If fat grafting is to become a useful and validated clinical tool, these issues must be addressed.…”

Section: Biological Properties Of Fat Graft and Adscsmentioning

confidence: 99%

“…In contrast, Butala et al suggested that introduced ADSCs may recruit further stem cells, particularly from bone marrow ( 30 , 53 , 55 , 72 ). To enhance the relative ADSC abundance within fat grafts ( 44 , 58 , 72 , 73 ), Yoshimura et al proposed “cell-assisted lipotransfer enrichment” (Figure 2 ), by supplementing lipoaspirate with additional SVF ( 23 , 43 , 60 , 62 , 64 , 74 , 75 ). The SVF [comprised 10% ADSCs ( 45 , 46 , 65 , 67 )], is obtained from a component of lipoaspirate, surplus to the volume anticipated to be required to fill a known defect ( 30 ).…”

Section: Enhancing Fat Graft Take Using Adscsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Radiotherapy Injury: A New Frontier

2015

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Radiotherapy is increasingly used to treat numerous human malignancies. In addition to the beneficial anti-cancer effects, there are a series of undesirable effects on normal host tissues surrounding the target tumor. While the early effects of radiotherapy (desquamation, erythema, and hair loss) typically resolve, the chronic effects persist as unpredictable and often troublesome sequelae of cancer treatment, long after oncological treatment has been completed. Plastic surgeons are often called upon to treat the problems subsequently arising in irradiated tissues, such as recurrent infection, impaired healing, fibrosis, contracture, and/or lymphedema. Recently, it was anecdotally noted – then validated in more robust animal and human studies – that fat grafting can ameliorate some of these chronic tissue effects. Despite the widespread usage of fat grafting, the mechanism of its action remains poorly understood. This review provides an overview of the current understanding of: (i) mechanisms of chronic radiation injury and its clinical manifestations; (ii) biological properties of fat grafts and their key constituent, adipose-derived stem cells (ADSCs); and (iii) the role of ADSCs in radiotherapy-induced soft-tissue injury.

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“…ADSCs have now become the most studied adult stem cell and are thought to play a key role in the restorative, reconstructive and sustainable qualities of transferred fat 16 34––38…”

Section: Introductionmentioning

confidence: 99%

Use of autologous fat grafting for reconstruction postmastectomy and breast conserving surgery: a systematic review protocol

2013

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IntroductionThere is growing interest in the potential use of autologous fat grafting (AFG) for the purposes of breast reconstruction. However, concerns have been raised regarding the technique's clinical effectiveness, safety and interference with screening mammography. The objective of this systematic review was to determine the oncological, clinical, aesthetic and functional, patient reported, process and radiological outcomes for AFG.Methods and analysisAll original studies, including randomised controlled trials, cohorts studies, case–control studies, case series and case reports involving women undergoing breast reconstruction. All AFG techniques performed for the purposes of reconstruction in the postmastectomy or breast conserving surgery setting will be considered. Outcomes are defined within this protocol along; oncological, clinical, aesthetic and functional, patient reported, process and radiological domains. The search strategy has been devised to find papers about ‘fat grafting and breast reconstruction’ and is outlined within the body of this protocol. The full search strategy is outlined within the body of the protocol. The following electronic databases will be searched from 1 January 1986 to 6 June 2013: PubMed, MEDLINE, EMBASE, SCOPUS, CINAHL, PsycINFO, SciELO, The Cochrane Library, including the Cochrane Database of Systematic Reviews (CDSR), Cochrane Central Register of Controlled Trials (CENTRAL), Database of Abstracts of Reviews of Effect (DARE), the Cochrane Methodology Register, Health Technology Assessment Database, the NHS Economic Evaluation Databases and Cochrane Groups, ClinicalTrials.gov, Current Controlled Trials Database, the World Health Organisation (WHO) International Clinical Trials Registry Platform, UpToDate.com, NHS Evidence and the York Centre for Reviews and Dissemination. Grey literature searches will also be conducted as detailed in our review protocol. Eligibility assessment occurred in two stages, title and abstract screening and then full text assessment. Data were extracted and stored in a database with standardised extraction fields to facilitate easy and consistent data entry.Ethics and disseminationThis systematic review will be published in a peer-reviewed journal. It will also be presented at national and international conferences in the fields of plastic, reconstructive and aesthetic surgery and at more general surgical and methodological conferences. It will be disseminated electronically and in print. Brief reports of the review findings will be disseminated directly to the appropriate audiences of surgeons and societies through email and other modes of communication. Updates of the review will be conducted to inform and guide healthcare practice and policy.Protocol RegistrationPROSPERO—National Institute of Health Research (NIHR) Prospective Register of Systematic Reviews (CRD42013005254)

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Analysis for Apoptosis and Necrosis on Adipocytes, Stromal Vascular Fraction, and Adipose-Derived Stem Cells in Human Lipoaspirates after Liposuction

Abstract: Adipose-derived stem cells had a higher rate of apoptosis and necrosis than adipocytes. However, the extent of apoptosis and necrosis was significantly different among adipose-derived stem cell subpopulations.

Cited by 29 publications

References 33 publications

The Effect of Antiseptics on Adipose-Derived Stem Cells

The Effect of Antiseptics on Adipose-Derived Stem Cells

Adipose-Derived Stem Cells in Radiotherapy Injury: A New Frontier

Use of autologous fat grafting for reconstruction postmastectomy and breast conserving surgery: a systematic review protocol

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