Analysis of 41 kb of the DNA sequence from the right arm of chromosome II of Schizosaccharomyces pombe

Abstract

We report the complete sequence of cosmid c18A7 (41 046 bp insert), located on the right arm of chromosome II of the Schizosaccharomyces pombe genome. The sequence, which partially overlaps with cosmids SPBC4F6 and SPBC336, contains 16 open reading frames (ORFs) capable of coding for proteins of at least 100 amino acid residues in length (one partial) and one small nucleolar RNA (snoRNA). Four known genes were found: swi10 (encoding a mating-type switching protein also involved in nucleotide excision repair); dim1 (encoding a dimethyladenosine transferase); arf1 (encoding ADP-ribosylation factor 1); and pol3 (cdc6) the partial fragment, encoding the 125 kDa catalytic subunit of the DNA polymerase type B. Six ORFs similar to known proteins were found. They include a transporter of the major facilitator superfamily class, a vacuolar sorting protein, an asparagine synthase, a nuclear protein, a reticulum oxidoreductin and a heat shock protein.Each protein product of the other six ORFs has conserved domains and can be assigned a molecular, but not a biological, function. The sequence has been submitted to the EMBL database under Accession No. AL080287.

Identification and validation of reference genes for gene expression analysis in Aphidius gifuensis (Hymenoptera: Aphidiidae)

“…Collectively, the results of our study have validated the findings of earlier reports that no single reference gene is stably expressed in all treatments [ 8 , 27 , 30 , 43 ]. The novel reference gene, DIMT , which encodes a dimethyladenosine transferase that, specifically dimethylates two adjacent adenosines and is situated within a conserved hairpin loop near the 3′-end of the 18S rRNA of the 40S ribosomal subunit[ 44 ], is differentially expressed among different developmental stages and sexes. The present study has also determined that RPII3 should be excluded as a stably expressed reference gene, similar to the findings of a previous study involving Raphanus sativus L.[ 45 ].…”

Characterization of a chaperone ClpB homologue ofParacoccidioides brasiliensis

“…It is shown a high number of identical and conserved amino acids among the considered sequences of the ClpB from Escherichia coli (Gottesman et al, 1990), Synechococcus sp. (Eriksson and Clarke, 1996), Schizosaccharomyces pombe (Sanches et al, 2001) and Saccharomyces cerevisiae (Parsell et al, 1991). Of special note is the conservation of the ATPbinding motifs 1 and 2, which is typical of ClpB sequences.…”

“…The sequences were: Escherichia coli (Gottesman et al, 1990), Synechococcus sp. (Eriksson and Clarke, 1996), Schizosaccharomyces pombe (Sanches et al, 2001) and Saccharomyces cerevisiae (Parsell et al,1991). Asterisks indicate amino acid identity and dots represent conserved substitutions.…”

Quantitative proteomic analysis of chromatin-bound proteins in the fission yeast Schizosaccharomyces pombe

“…Previous studies suggested that Sec6 is present in SPB and crucial for cytokinetic cell separation in S. pombe (490). An unexpected finding was Tpi1, which is a triosephosphate isomerase involving in various biosynthetic events (491). Other findings were more unexpected, including Cc736.03c, Psd3 and Cc1183.11.…”