Analysis of a 103 kbp cluster homology region from the left end ofSaccharomyces cerevisiaechromosome I

Abstract

The DNA sequence and preliminary functional analysis of a 103-kbp section of the left arm of yeast chromosome I is presented. This region, from the left telomere to the LTE1 gene, can be divided into two distinct portions. One portion, the telomeric 29 kbp, has a very low gene density (only five potential genes and 21 kbp of noncoding sequence), does not encode any "functionally important" genes, and is rich in sequences repeated several times within the yeast genome. The other portion, with 37 genes and only 14.5 kbp of noncoding sequence, is gene rich and codes for at least 16 "functionally important" genes. The entire gene-rich portion is apparently duplicated on chromosome XV as an extensive region of partial gene synteney called a cluster homology region. A function can be assigned with varying degrees of precision to 23 of the 42 potential genes in this region; however, the precise function is know for only eight genes. Nineteen genes encode products presently novel to yeast, although five of these have homologs elsewhere in the yeast genome.

The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting

“…Saccharomyces has undergone a genome duplication sometime after it diverged from another Ascomycete, Kluyveromyces (Wolfe and Shields, 1997), and MYO2 and MYO4 lie in an unambiguously duplicated region ( Fig. 8 A) (Parle-McDermott et al, 1996;Pearson et al, 1996;Storms et al, 1997). In support of a recent duplication, the Myo2p and Myo4p tails are more similar to each other than to any other myosin V. Furthermore, phylogenetic analysis of motor domain sequences (Fig.…”

Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

“…In several cases, haploid null mutants were reported to have slow-growth phenotypes [ ymr308c (PSE1), yol022c, ypl243c (srp68), ypl210c (srp72), and ydr353w (trr1) (25)] or to be temperature sensitive [ ydr113c ( pds1) and ygr216c ( gpi1) (26)]. In some cases, haploid deletions strains were constructed for genes previously determined to be essential by others [HKR1, RNR1, and FUN9 (27)]. (B) Distribution of functional classes of essential (inner circle) and nonessential (outer circle) ORFs using criteria from the Munich Information Centre for Protein Sequences (MIPS) (10).…”