2021
DOI: 10.3390/ijms22031163
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Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

Abstract: Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important ro… Show more

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Cited by 2 publications
(2 citation statements)
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References 114 publications
(179 reference statements)
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“…The most frequently used CSC isolation technique in osteosarcoma was Fluorescent-Activated Cell Sorter (FACS) combined with subsequent culture of the isolated and sorted cells in induction media for maintenance [25,27,31,37,46]. Four articles carried out the sphere formation assay using an induction culture medium [24,28,35,36], one article used FACS only without induction medium [33], two articles used the Magnetic-Activated Cell Sorting (MACS) technique and then induction medium [26,29], one article used MACS without induction medium [34], and finally, the Side Population (SP) technique with subsequent induction medium was used in three articles [13,30,32].…”
Section: Bone Sarcomamentioning
confidence: 99%
See 1 more Smart Citation
“…The most frequently used CSC isolation technique in osteosarcoma was Fluorescent-Activated Cell Sorter (FACS) combined with subsequent culture of the isolated and sorted cells in induction media for maintenance [25,27,31,37,46]. Four articles carried out the sphere formation assay using an induction culture medium [24,28,35,36], one article used FACS only without induction medium [33], two articles used the Magnetic-Activated Cell Sorting (MACS) technique and then induction medium [26,29], one article used MACS without induction medium [34], and finally, the Side Population (SP) technique with subsequent induction medium was used in three articles [13,30,32].…”
Section: Bone Sarcomamentioning
confidence: 99%
“…The most frequently used base medium was DMEM/F12, with a total of nine articles. One article used Ham's F12 [24], while DMEM [27], RPMI [35], and N2B27 [37] were used depending on the cell line.…”
Section: Bone Sarcomamentioning
confidence: 99%