Analysis of absorption-enhancing mechanisms for combinatorial use of spermine with sodium taurocholate in Caco-2 cells

Maruyama, Masato; Nishida, Yohei; Tanaka, Hironori; Minami, Takako; Ogawara, Ken Ichi; Miyake, Masaaki; Takamura, Yuta; Kakuta, Hiroki; Higaki, Kazutaka

doi:10.1016/j.ejpb.2022.10.020

Cited by 7 publications

(3 citation statements)

References 52 publications

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“…111 A recent report has shown an increase in fluidity and tight junction opening of Caco-2 cells for labeled dextran when a combination of spermine and sodium taurocholate was used. 112 Several reports have suggested a maximum opening of tight junction space of up to 20 nm, including the use of enhancers. 113 Thus, the transport of larger particles through the paracellular pathway is still debatable.…”

Section: Can Orally Delivered Nanoparticles Bypass Cellular Uptake?mentioning

confidence: 99%

“…High bioavailability of oral cephalexin was observed under conditions of excessive 5-hydroxy-triptamine serotonin via the expanded paracellular route . A recent report has shown an increase in fluidity and tight junction opening of Caco-2 cells for labeled dextran when a combination of spermine and sodium taurocholate was used …”

Section: Can Orally Delivered Nanoparticles Bypass Cellular Uptake?mentioning

confidence: 99%

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Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

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Oral drug administration has been a popular choice due to patient compliance and limited clinical resources. Orally delivered drugs must circumvent the harsh gastrointestinal (GI) environment to effectively enter the systemic circulation.The GI tract has a number of structural and physiological barriers that limit drug bioavailability including mucus, the tightly regulated epithelial layer, immune cells, and associated vasculature. Nanoparticles have been used to enhance oral bioavailability of drugs, as they can act as a shield to the harsh GI environment and prevent early degradation while also increasing uptake and transport of drugs across the intestinal epithelium. Evidence suggests that different nanoparticle formulations may be transported via different intracellular mechanisms to cross the intestinal epithelium. Despite the existence of a significant body of work on intestinal transport of nanoparticles, many key questions remain: What causes the poor bioavailability of the oral drugs? What factors contribute to the ability of a nanoparticle to cross different intestinal barriers? Do nanoparticle properties such as size and charge influence the type of endocytic pathways taken? In this Review, we summarize the different components of intestinal barriers and the types of nanoparticles developed for oral delivery. In particular, we focus on the various intracellular pathways used in nanoparticle internalization and nanoparticle or cargo translocation across the epithelium. Understanding the gut barrier, nanoparticle characteristics, and transport pathways may lead to the development of more therapeutically useful nanoparticles as drug carriers.

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Section: Can Orally Delivered Nanoparticles Bypass Cellular Uptake?mentioning

confidence: 99%

Section: Can Orally Delivered Nanoparticles Bypass Cellular Uptake?mentioning

confidence: 99%

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

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“…Results of our own studies also suggest that the ‘normalization’ of arginase activity was associated with a decrease in MLCK protein [ 84 ]. It has been shown that MLCK activity may be induced in cells treated with spermine [ 94 ], the latter being a downstream substrate of arginase-mediated formation of ornithine. However, if the lower arginase activity found in small intestinal tissue exposed to fructose alters spermine bioavailability, and hereby, MLCK activity remains to be determined.…”

Section: Fructose Intestinal Microbiota and Intestinal Barriermentioning

confidence: 99%

Fructose: a modulator of intestinal barrier function and hepatic health?

Staltner,

Burger,

Baumann

et al. 2023

Eur J Nutr

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Purpose Consumption of fructose has repeatedly been discussed to be a key factor in the development of health disturbances such as hypertension, diabetes type 2, and non-alcoholic fatty liver disease. Despite intense research efforts, the question if and how high dietary fructose intake interferes with human health has not yet been fully answered. Results Studies suggest that besides its insulin-independent metabolism dietary fructose may also impact intestinal homeostasis and barrier function. Indeed, it has been suggested by the results of human and animal as well as in vitro studies that fructose enriched diets may alter intestinal microbiota composition. Furthermore, studies have also shown that both acute and chronic intake of fructose may lead to an increased formation of nitric oxide and a loss of tight junction proteins in small intestinal tissue. These alterations have been related to an increased translocation of pathogen-associated molecular patterns (PAMPs) like bacterial endotoxin and an induction of dependent signaling cascades in the liver but also other tissues. Conclusion In the present narrative review, results of studies assessing the effects of fructose on intestinal barrier function and their impact on the development of health disturbances with a particular focus on the liver are summarized and discussed.

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Absorption enhancement of peach kernel oil on hydroxysafflor yellow A in safflower extracts and its mechanisms

Ye,

Tang,

Tao

et al. 2024

Food Chemistry

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Analysis of absorption-enhancing mechanisms for combinatorial use of spermine with sodium taurocholate in Caco-2 cells

Cited by 7 publications

References 52 publications

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

Fructose: a modulator of intestinal barrier function and hepatic health?

Absorption enhancement of peach kernel oil on hydroxysafflor yellow A in safflower extracts and its mechanisms

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