Analysis of amlodipine in human plasma by gas chromatography

Beresford, Alan; Macrae, P.V.; Stopher, D. A.; Wood, Brian

doi:10.1016/0378-4347(87)80170-6

Cited by 72 publications

(24 citation statements)

References 4 publications

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“…They had no history of serious ill-health and none was taking any regular medication including overthe-counter preparations during 1 Blood samples for the measurement of plasma concentrations of drugs were taken before and after each infusion study. Plasma concentrations of amlodipine were measured by gas chromatography with electron capture [14], with inter-and intra-assay coefficients of variation of 6.7 and 5.6%, respectively, and a limit of detection of 0.5 ng ml-'. Plasma concentrations of nifedipine were measured by modified high performance liquid chromatography [15] using ultraviolet detection, with inter-and intra-assay coefficients of variation of 7.3% and 6.1%, respectively, and a limit of detection of 3.0 ng ml-'.…”

Section: Subjectsmentioning

confidence: 99%

A comparative assessment of the duration of action of amlodipine and nifedipine GITS in normotensive subjects

Ueda

Meredith

Howie

et al. 1993

Brit J Clinical Pharma

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1 This study in normotensive subjects compared the duration and consistency of action of amlodipine (5 mg) and nifedipine GITS (60 mg) by assessment of the attenuation of pressor responses to noradrenaline and angiotensin II. 2 Both drugs significantly attenuated pressor responses to both vasoconstrictors at 6and 24 h post-dose with rightward shifts of up to 2.3-fold in the dose-response curves. 3 There was significantly less pharmacokinetic variability with amlodipine: for example, intra-subject variability was 33% with amlodipine and 59% with nifedipine GITS. 4 There were no significant differences in the pressor dose ratios up to 48 h post-dose with amlodipine whereas there was a significant and progressive reduction in the pressor dose ratios with nifedipine. 5 These results suggest that both drugs are broadly comparable as once daily treatments but amlodipine displayed less intra-and inter-subject variability and provided a significantly more sustained effect with a reserve of pharmacological activity up to 48 h post-dose.

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Section: Subjectsmentioning

confidence: 99%

A comparative assessment of the duration of action of amlodipine and nifedipine GITS in normotensive subjects

Ueda

Meredith

Howie

et al. 1993

Brit J Clinical Pharma

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“…ADB is a calcium channel blocker with a vasodilatory activity similar to that of nifedipine and is mainly used for its antianginal, antihypertensive and antiarrhythmic activities [2][3][4] . ADB in biological fluids has been determined mostly by chromatographic techniques such as gas chromatography, 5 liquid chromatography-tandem mass spectrometry (LC-TMS) [6][7][8][9] , high performance liquid chromatography (HPLC) [10][11][12][13] and high performance thin layer chromatography (HPTLC) 14 . A limited number of methods have been reported for the quantitation of this drug when present alone in dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Basavaiah¹,

Chandrashekar²,

Nagegowda³

2006

ScienceAsia

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ABSTRACT:One direct titrimetric method and two indirect spectrophotometric methods for the determination of amlodipine besylate (ADB) are described. The methods use bromate-bromide mixture and two-dyes, namely Methyl Orange and Indigo Carmine, as reagents. In titrimetry (Method A), an acidified solution of ADB is titrated directly with bromate-bromide mixture using methyl orange as an indicator. Spectrophotometry involves the addition of known excess of bromate-bromide mixture to an acidified solution of ADB and the determination of the residual bromine based on its ability to bleach the dyes Methyl Orange (Method B) or Indigo Carmine (Method C) quantitatively. Titrimetry allows the determination of 1-10 mg of ADB, whereas spectrophotometry is applicable over the concentration ranges of 0.5-3.0 µg ml -1 and 1.25-12.50 µg ml -1 for method B, and method C, respectively. Method B with a calculated molar absorptivity of 6.56 x 10 4 l mol -1 cm -1 , is the most sensitive method ever developed for ADB. The limits of detection and quantification are reported for both the spectrophotometric methods. The methods described could usefully be applied to routine quality control of tablets containing ADB. No interference was observed from common pharmaceutical adjuvants. Statistical comparison of the results with the reference method shows an excellent agreement, and indicates no significant difference in accuracy and precision. The reliability of the methods has been ascertained by recovery studies.

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“…4 A detailed literature survey for AB revealed that several analytical methods are reported for the determination of amlodipine with atorvastatine by HPLC, 5 amlodipine with atenolol by HPLC 6 and by HPTLC, 7 amlodipine with metaprolol by HPLC 8 and amlodipine alone by HPLC, 9 HPTLC, 10 LC-MS 11 and GC. 12 Similarly, a literature survey for BH revealed that few techniques have been reported for the determination of BH alone by HPLC, 13 HPTLC, 14 CE, 15 GC-MS, 16 LC-MS, 17 spectrophotometric detection 18 and by voltammetrically 19 from pharmaceutical dosage form. Only two methods have been reported for the simultaneous determination of AB and BH; one describes the RP-HPLC procedure 20 and the other describes HPTLC procedure, 21 but both these methods are time-consuming.…”

Section: Benazepril 2-[(3s)-3-[[(2s)-1-ethoxy-1-oxo-4-phenylbutan-2-mentioning

confidence: 99%

Simultaneous Determination of Amlodipine Besylate and Benazepril Hydrochloride in Pharmaceutical Dosage Form by LC

Kasawar

Farooqui

2009

ANAL. SCI.

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Analysis of amlodipine in human plasma by gas chromatography

Cited by 72 publications

References 4 publications

A comparative assessment of the duration of action of amlodipine and nifedipine GITS in normotensive subjects

A comparative assessment of the duration of action of amlodipine and nifedipine GITS in normotensive subjects

Untitled

Simultaneous Determination of Amlodipine Besylate and Benazepril Hydrochloride in Pharmaceutical Dosage Form by LC

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