Analysis of Anisodine and Identification of Twenty of Its Metabolites in Rat Urine by Liquid Chromatography–Tandem Mass Spectrometry

Structural elucidation of in vivo and in vitro metabolites of anisodine by liquid chromatography–tandem mass spectrometry

“…Based on the above discussion and combining the results we have reported [5], the proposed metabolic pathway of anisodine in rats is presented in Fig. 2. …”

“…Despite its important therapeutic value, its in vivo or in vitro metabolism is not clear yet. Up till now, the works mainly focused on the quantitative determination of anisodine in plant [3,4] and only two recent publications from our group deal with the metabolism of anisodine [5,6].…”

“…In the course of our recent study [5,6] 20 metabolites were detected and identified by LC-MS n in rat urine and four metabolites in rat plasma. The major metabolic pathway of anisodine in rat was hydrolysis, demethylation, dehydration, hydroxylation, and sulfate and glucuronide conjugation.…”

Discovery of new muscarinic acetylcholine receptor antagonists from Scopolia tangutica

“…Furthermore, six tropane compounds and three cinnamic acid amides were discovered from S. tangutica for the first time. They are characterized as dihydroanisodamine ( 5 ), dihydroanisodine ( 6 ), noranisodine31 ( 7 ), noranisodamine ( 9 )32, deepoxyanisodine33 ( 10 ), norhyoscyamine ( 11 )3334, N-trans-p- Coumaroylputrescine3536 ( 13 ), N-trans-isoferuloylputrescine3738 ( 15 ) and N-cis- isoferuloylputrescine38 ( 16 ), based on their mass fragment and NMR spectrum. The elucidation and specific assignments of these compounds were in supporting information.…”

Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase

“…anisodine [1] and hydrophilic synthetic intermediates of basic drugs, e.g. cytosine and serotonin [2], receive much attention due to their biomedical applications.…”