Analysis of Antigenotoxicity of Lactobacillus Salivarius by High Performance Liquid Chromatography

Wang, Fang; Jiang, Lu; Liu, Aiping; Guo, Xinghua; Ren, Fazheng

doi:10.1016/s1872-2040(08)60040-7

Cited by 19 publications

(9 citation statements)

References 11 publications

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“…Coincubation was protracted under anaerobic conditions for different period of time and with agitation (200 rpm, 37 C). The supernatant was recovered by centrifugation, filtered using a 0.45 mm membrane, and analyzed by highperformance liquid chromatography (HPLC) as described in Wang and colleagues (19).…”

Section: Hplc Analysis Of 4nqo Metabolism In Vitromentioning

confidence: 99%

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Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

Zhang

Wang

Jiang

et al. 2013

Cancer Prevention Research

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Despite significant advances in cancer therapy, cancer-related mobility and mortality are still rising. Alternative strategies such as cancer prevention thus become essential. Probiotics represent an emerging option for cancer prevention, but studies are limited to colon cancers. The efficiency of probiotics in the prevention of other cancers and the correlative mechanism remains to be explored. A novel probiotics Lactobacillus salivarius REN (L. salivarius REN) was isolated from centenarians at Bama of China, which showed highly potent antigenotoxicity in an initial assay. 4-nitroquioline 1-oxide (4NQO)-induced oral cancer model was introduced to study the anticancer activity of L. salivarius REN in vivo. The results indicated that oral administration of probiotic L. salivarius REN or its secretions could effectively suppress 4NQO-induced oral carcinogenesis in the initial and postinitial stage, and the inhibition was in a dose-dependent manner. A significant decrease of neoplasm incidence (65%-0%) was detected in rats fed with the high dose of L. salivarius REN [5 Â 10 10 CFU/kg body weight (bw)/d]. In vivo evidences indicated that the probiotics inhibited 4NQO-induced oral cancer by protecting DNA against oxidative damage and downregulating COX-2 expression. L. salivarius REN treatment significantly decreased the expression of proliferating cell nuclear antigen (PCNA) and induced apoptosis in a dose-dependent manner. Our findings suggest that probiotics may act as potential agents for oral cancer prevention. This is the first report showing the inhibitory effect of the probiotics on oral carcinogenesis. Cancer Prev Res; 6(7); 686-94. Ó2013 AACR.

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Section: Hplc Analysis Of 4nqo Metabolism In Vitromentioning

confidence: 99%

“…In a previous study, it was found that L. salivarius REN could decrease the 4-nitroquioline 1-oxide (4NQO)-induced genotoxicity in vitro (19). To further verify these potential anticarcinogensis effect in vivo, inhibitory activity of L. salivarius REN on 4NQO-induced oral carcinogenesis was investigated in male F344 rats.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

Zhang

Wang

Jiang

et al. 2013

Cancer Prevention Research

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“…For instance, L. salivarius CECT 5713 was extensively studied not only in vitro assays but also in animal studies and clinical trials, demonstrating its functional benefits, particularly the antiinflammatory effects [5], and L. salivarius UCC118 could reduce Listeria infection in mice [6]. Recently, Wang et al [7] reported that L. salivarius FDB89 was able to convert the carcinogen of 4-nitroquinoline-N-oxide (4-NQO) and possesses the antigenotoxic activity against 4-NQO. Besides, Zhang et al [8] demonstrated its property of L. salivarius FDB89 in colonic microflora of rats.…”

Section: Introductionmentioning

confidence: 99%

Cell surface properties of Lactobacillus salivarius under osmotic stress

Gong

Chen

et al. 2012

Eur Food Res Technol

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The modifications of cell morphology and surface properties of Lactobacillus salivarius under osmotic stress were investigated. When deMan-RogosaSharpe (MRS) medium was supplemented with NaCl (0.8 mol l -1 ), changes in cell size and shape of L. salivarius cells occurred. A higher autoaggregation percentage and a lower hydrophobicity of L. salivarius determined by microbial adhesion to hydrocarbons were observed under osmotic stress. Moreover, coaggregation was strain specific and depended on incubation time as well as growth medium. On the other hand, fatty acid analysis by gas chromatograph-mass spectrometer revealed increases in the ratio of saturated/unsaturated fatty acids and cyclopropaneoctanoic acid, which is a useful way to enhance their osmotic resistance for maintaining the structural and functional integrity of cell membranes.

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“…LsR was isolated from the fecal of a healthy centenarian living in Bama, Guangxi, China. This strain decreases 4-NQO-induced genotoxicity in vitro (Wang et al, 2008). Moreover, it suppresses 4-NQO-induced oral carcinogenesis and DMH-induced colorectal carcinogenesis in vivo (Zhang et al, 2013b;Zhu et al, 2014).…”

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confidence: 84%

“…Our previous study showed that LsR inhibited 4-NQO genotoxicity by degrading its metabolin, 4-hydroxyaminoquinoline 1-oxide (4-HAQO), which could damage DNA (Wang et al, 2008). Hydrogen peroxide (H 2 O 2 ) secreted by LsR plays an essential role in degrading 4-HAQO (unpublished data).…”

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confidence: 99%

Complete genome sequence of Lactobacillus salivarius Ren, a probiotic strain with anti-tumor activity

Sun

Ren

Liu

et al. 2015

Journal of Biotechnology

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Analysis of Antigenotoxicity of Lactobacillus Salivarius by High Performance Liquid Chromatography

Cited by 19 publications

References 11 publications

Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

Cell surface properties of Lactobacillus salivarius under osmotic stress

Complete genome sequence of Lactobacillus salivarius Ren, a probiotic strain with anti-tumor activity

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