Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

Abstract

Despite significant advances in cancer therapy, cancer-related mobility and mortality are still rising. Alternative strategies such as cancer prevention thus become essential. Probiotics represent an emerging option for cancer prevention, but studies are limited to colon cancers. The efficiency of probiotics in the prevention of other cancers and the correlative mechanism remains to be explored. A novel probiotics Lactobacillus salivarius REN (L. salivarius REN) was isolated from centenarians at Bama of China, which showed highly potent antigenotoxicity in an initial assay. 4-nitroquioline 1-oxide (4NQO)-induced oral cancer model was introduced to study the anticancer activity of L. salivarius REN in vivo. The results indicated that oral administration of probiotic L. salivarius REN or its secretions could effectively suppress 4NQO-induced oral carcinogenesis in the initial and postinitial stage, and the inhibition was in a dose-dependent manner. A significant decrease of neoplasm incidence (65%-0%) was detected in rats fed with the high dose of L. salivarius REN [5 Â 10 10 CFU/kg body weight (bw)/d]. In vivo evidences indicated that the probiotics inhibited 4NQO-induced oral cancer by protecting DNA against oxidative damage and downregulating COX-2 expression. L. salivarius REN treatment significantly decreased the expression of proliferating cell nuclear antigen (PCNA) and induced apoptosis in a dose-dependent manner. Our findings suggest that probiotics may act as potential agents for oral cancer prevention. This is the first report showing the inhibitory effect of the probiotics on oral carcinogenesis. Cancer Prev Res; 6(7); 686-94. Ó2013 AACR.

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

“…32 Therefore, combining the knowledge that the presence of host CD24 modulates microbial activity in the oral cavity, and that patients with oral cancer have dysbiosis, it is possible that disruption of the CD24-oral microbiome axis alters cancer development. 33,34 Zhang et al 35 demonstrated that probiotic L. salivarius REN treatment significantly damped 4-NQO-induced oral cancer development in rats; however, the significance of the oral microbiome in 4-NQO-induced oral carcinogenesis has never been addressed in mouse models. Further, due to the known role of bacteria-produced sialidase in disrupting the CD24-Siglec-G/10 connection, we investigated if CD24 deficiency alters intra-oral microflora in a manner that may contribute to oral carcinogenesis.…”

Probiotikler ve Diş Hekimliğinde Kullanımı

“…It also significantly decreased the expression of proliferating cell nuclear antigen (PCNA), and induced apoptosis in a dose-dependent manner. The findings of this study suggest that probiotics may act as potential agents for oral cancer prevention 55 .…”

Modification in the diet can induce beneficial effects against breast cancer

“…This effect was attributed to an increased antioxidant status and decreased expression of oncogenes [98] . The beneficial effects of LAB were also reported in animal models of oral cancer [99] , and skin carcinogenesis [100] .…”