Analysis of antimicrobial susceptibility pattern of ocular infections at Regional Ophthalmic Institute in India

Nuzhat, Qudsia; Bandyopadhyay, Arkapal; Chaurasia, Rajan; Singh, Satya Prakash; Singh, Monica

doi:10.18203/2319-2003.ijbcp20201192

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…This may be attributed to their outdoor activities as people within this age range make up majority of the labor force. Similar result was seen in the study conducted in India [13]. More so, patients of low socio-economic group like farmers, stone quarriers, metal miners had a highest prevalence of ocular infections of (62.2%), (73.8%) and (71%) respectively.…”

Section: Discussionsupporting

confidence: 87%

Fungal Organisms Associated with Ocular Infections in a Tertiary Care Hospital

2022

OAJMMS

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Background: Ocular infections in man are the contamination and invasion of ocular tissues by micro-organisms leading to the breakdown of the natural defense mechanisms of the eyes. The areas in the eye that are frequently infected are the conjunctiva, lid and cornea. Aim: This study was undertaken to determine the fungal organisms associated with Ocular Infections in Tertiary Care Hospitals in Abia State. Methods: Ocular specimens were collected from 500 patients and investigated using culture techniques. Results: The five hundred (500) patients, thirty-seven 37(7.4%) had fungal contamination. There was a statistical significance (p=0.006) in sex as males had 22(8.4%) more fungal contamination while females had 15(6.3%). Age played a significant role (P=0.001) as Individuals between 30-45 years were more affected (13.1%) while the least was in the age group 0-15 years (1.4%). The occupation of the patients significantly influenced (P=0.002) the prevalence of the fungal contamination of the eyes as individuals who were engaged in farming activities had the highest contamination 11(12.2%) followed by stone quarrying activities 9(13.8%). The least came from the civil servants 2(2.5%). Aspergillus niger 40(8.0%) was the most prevalent, followed by Candida albicans 32(6.4%) while Aspergillus fumigatus had the least 22(4.4%). Aspergillus flavus and Fusarium spp prevalence were 28(5.6%) and 27(5.4%) respectively. Conclusions: The culture positivity in this study was very high. Fungal infections of the eye were mostly caused by Aspergillus Niger, Aspergillus flavus, Aspergillus fumigatus, C. albicans and Fusarium spp.

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Section: Discussionsupporting

confidence: 87%

Fungal Organisms Associated with Ocular Infections in a Tertiary Care Hospital

2022

OAJMMS

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Bacteriological Profile and Antimicrobial Susceptibility Pattern of Isolates From Ocular Infections Among the Patients Attending Ophthalmology Department of a Tertiary Care Hospital.

asasmy

Shambhavi

Rao

2021

IJAR

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Background: Ocular infections are one of the major causes of visual impairment around the world. Infections can either be due to single microbe or polymicrobial. it is essential to know the specic etiology of the infection for the effective management of ocular infections. This study aims to identify microbes responsible for common ocular infections and to determine its antibiotic susceptibility to commonly used antibiotics used in clinical practice. Clinical Methods: A hospital based cross sectional study included 290 patients attending the ophthalmology department of a tertiary care hospital. After a thorough clinical examination samples were taken and were subjected to Grams' stain, culture methods, biochemical tests for the identication of the microbe. Antibacterial susceptibility test was performed using Kirby Bauer disc diffusion method. Results: Among 290 patients, the common clinical conditions include conjunctivitis 108 (37.24%), keratitis 37 (12.76%), dacryocytitis 53 (18.28%), blepharitis 52 (17.93%), trauma 24 (8.28%), infective uveitis 10(3.45%) and endophthalmitis 6(2.07%). Among the total ocular infections 146(50.34%) were positive for culture. Among the total isolated, 110(75.34%) were Gram positive and 36(24. 66%) were Gram negative. The Gram positive bacteria isolated showed highest sensitivity to ciprooxacin (86.36%) followed by gentamycin (82.72%) and clindamycin (80%). The Gram negative bacteria showed high resistance to tetracycline 7(19.44%), followed by amoxicillin-clavulanic acid (30.56%). Conclusion: The most common ocular infection is conjunctivitis followed by dacryocystitis which is most commonly caused by Staphylococcus aureus, which showed high resistance to Amoxcillin, Tetracycline and Erythromycin. To prevent the emergence of antimicrobial resistance, it is necessary to perform antimicrobial susceptibility testing before initiating antibiotics in clinical practice.

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Antibiotic Susceptibility Profile and Plasmid Analysis of Micro-Organisms Associated with Ocular Infections

Unegbu

Anyamene

Odibo

2022

Biomedical and Biotechnology Research Journal (BBRJ)

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Background: This study was undertaken to determine the antimicrobial susceptibility profile and plasmid analysis of micro-organisms associated with ocular infections. Methods: Ocular specimens were collected from 500 patients. Subsequent identification was done based on morphology and biochemical tests. Susceptibility pattern of the isolates was done using the disk diffusion method. The presence of plasmids was determined using the agar gel electrophoresis technique. Plasmid curing was carried out by treating the MAR isolates with sodium dodecyl sulfate at concentration of 10%. Results: Conjunctivitis was the most prevalent ocular infection of 105 (39.8%). S. aureus was the most prevalent pathogen 63 (23.9%) followed by CoNS 36 (13.6%). S. aureus was 100% sensitive to vancomycin and chloramphenicol. CoNS was also 100% sensitive to ciprofloxacin, vancomycin, and chloramphenicol. K pneumoniae was 100% sensitive to gentamicin and amoxicillin-clavulanic acid while N. gonorrhoeae was 100% sensitive to gentamicin, ciprofloxacin, ceftriaxone, amoxicillin-clavulanic acid, and cefotaxime. The overall MAR bacteria was 38 (16.2%). Plasmid analysis revealed the presence of 1–3 plasmid bands of sizes 6.21–16.22 Kbp from the MAR isolates. Plasmid curing revealed that the gene coding for resistant seen in this study was plasmid mediated. Conclusions: The prevalence of ocular infection was high with conjunctivitis being the dominant. The dominant bacteria species were S. aureus and CoNS. The overall MAR bacteria proportion was relatively high. The presence of plasmids was responsible for the resistance of the isolates to antibiotics.

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Analysis of antimicrobial susceptibility pattern of ocular infections at Regional Ophthalmic Institute in India

Cited by 3 publications

References 10 publications

Fungal Organisms Associated with Ocular Infections in a Tertiary Care Hospital

Fungal Organisms Associated with Ocular Infections in a Tertiary Care Hospital

Bacteriological Profile and Antimicrobial Susceptibility Pattern of Isolates From Ocular Infections Among the Patients Attending Ophthalmology Department of a Tertiary Care Hospital.

Antibiotic Susceptibility Profile and Plasmid Analysis of Micro-Organisms Associated with Ocular Infections

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