Analysis of Biochemical and Clinical Pregnancy Loss Between Frozen-Thawed Embryo Transfer of Blastocysts and Day 3 Cleavage Embryos in Young Women: A Comprehensive Comparison

Dai, Xiuliang; Gao, Tingting; Xia, Xiyang; Cao, Fang; Yu, Chunmei; Li, Tianfu; Wang, Yufeng; Chen, Li

doi:10.3389/fendo.2021.785658

Cited by 10 publications

(4 citation statements)

References 46 publications

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“…With the constant advancements of the level of assisted reproductive technology, high-quality embryo obtainment is no longer a clinical problem. Nevertheless, the pregnancy rate of patients remains less than satisfactory, mainly due to poor endometrial receptivity [ 3 , 4 ]. Since the success of frozen-thawed human embryo transfer in Australia in 1983, frozen-thawed embryo transfer (FET) technology has been widely used in the field of human Assisted Reproductive Technology (ART) due to its high cumulative pregnancy rate.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Relevant Factors Affecting the Pregnancy Rate of Frozen-Thawed Embryo Transfer Cycle

Liu

Wang

Lü

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The aim of this study is to explore the relevant factors affecting the pregnancy rate of frozen-thawed embryo transfer cycle. Methods. The clinical data of 931 patients who underwent artificial cycle preparation for endometrial FET from April 2017 to November 2020 in the reproductive center of our hospital were retrospectively analyzed. Results. According to the pregnancy situation, the patients were divided into 450 cases of pregnancy and 481 cases of biochemical pregnancy. The univariate analysis of FET biochemical pregnancy showed that there were statistically significant differences between pregnancy and biochemical pregnancy in terms of years of infertility, age, endometrial thickness, P level, E2/P, and the number of high-quality embryos ( P < 0.05 ). Multivariate analysis of pregnancy showed that age <30 years was a protective factor for biochemical pregnancy and endometrial thickness <8 mm and E2/P < 0.3 were risk factors ( P < 0.05 ). Conclusion. The regulation of endometrial thickness and E2/P serves as the key of treatment for patients undergoing FET using artificial cycle preparation for endometrial transfer, and it contributes to improve the pregnancy rate; also, the patient's age is an important indicator influencing the pregnancy rate.

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Section: Introductionmentioning

confidence: 99%

Analysis of Relevant Factors Affecting the Pregnancy Rate of Frozen-Thawed Embryo Transfer Cycle

Liu

Wang

Lü

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…However, blastocysts could have better quality and higher euploidy because of a re-selection procedure that spans developmental blocks and eliminates embryos with poor developmental potential compared to that of cleavage embryos (52-54). These results indicate that the difference in pregnancy outcomes between the transfer of blastocysts and day 3 cleavage embryos may largely depend on the quality of the embryos transferred (59).…”

Section: Discussionmentioning

confidence: 87%

Association between duration of progesterone supplementation and clinical outcomes in artificial frozen-thawed embryo transfer cycles

Liu¹,

Zhou²,

Hu³

et al. 2023

Front. Endocrinol.

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ObjectiveThe administration of progesterone before transfer in hormone replacement treatment (HRT) is crucial for the clinical outcomes of frozen-thawed embryo transfer (FET), but the optimal duration of progesterone remains controversial. This study aimed to investigate the effect of the duration of progesterone administration on the clinical outcomes of FET cycles.MethodsThis prospective cohort study included 353 artificial FET cycles conducted at a reproductive medicine center between April and October 2021. The FET cycles were stratified into four groups based on the duration of progesterone supplementation before the procedure and the embryonic development stage: group P3 (73 patients) received intramuscular progesterone for 3 days and group P4 (87 patients) for 4 days before Day 3 frozen embryo transfer, group P5 (70 patients) for 5 days and group P6 (123 patients) for 6 days before frozen blastocyst transfer. This trial was performed using one or two vitrified embryo(s) when the endometrial thickness reached 7 mm after estrogen supplementation in an artificial cycle. The primary outcome was clinical pregnancy, and secondary outcomes included biochemical pregnancy, implantation, early pregnancy loss, and live births.ResultsThere were no significant differences in the demographic and clinical characteristics between the groups. No significant difference was observed in the clinical pregnancy rates between groups: 23/73 (31.5%) in group P3 vs 28/87 (32.2%) in group P4 (P = 0.927). Compared to group P5 (41/70, 58.6%), the clinical pregnancy rate was not significantly different in group P6 (77/123, 62.6%, P = 0.753). There was no significant difference in the implantation rates between groups: 33/136 (24.3%) in group P3 vs 34/166 (20.5%) in group P4 (P = 0.431), and 62/133 (46.6%) in group P5 vs 107/231 (46.3%) in group P6 (P = 0.956). The duration of progesterone supplementation (mean: 3.5 ± 0.5 days; range:3–4 days) before Day 3 frozen embryo transfer did not impact clinical pregnancy (odds ratio [OR] 1.048; 95% confidence interval [CI], 0.518–2.119). The duration of progesterone administration (mean: 5.6 ± 0.5 days; range:5–6 days) before frozen blastocyst transfer may not affect clinical pregnancy (OR 1.339; 95% CI, 0.717–2.497).ConclusionThere may be no significant correlation between the duration of progesterone supplementation and pregnancy outcomes in artificial FET cycles, although the clinical pregnancy rate was higher when progesterone supplementation was extended for one day before FET.

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“…Several studies [15,26] have reported that the miscarriage rates have no statistically signi cant differences between > 10-and 8-cell-derived blastocysts in the frozen embryo transfer group; however, comparable reduced miscarriage rates (4.3% vs. 13.5%; p = 0.04) were observed in the ≥ 10-cell and 8-cell groups during ET cycles [14]. Furthermore, some studies have reported that blastocysts arising from good-quality D3 embryos with high cell numbers are associated with lower miscarriage rates [28,29]. These observations to some extent agree with the results of our study.…”

Section: Discussionmentioning

confidence: 95%

Increased blastomere number in D3 embryos is associated with higher live birth rates in vitrified– thawed single blastocyst transfer cycles

Zheng,

Lin,

et al. 2024

Preprint

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Background The number of D3 embryo blastomeres affects pregnancy outcomes in patients undergoing cleavage-stage embryo transfer. However, the association between blastomere number in D3 embryos and pregnancy outcomes after vitrified–thawed single blastocyst transfer remains unknown.Methods This retrospective cohort follow-up study included 2,274 cases of vitrified–thawed single blastocyst transfer,all patients were divided into six groups according to blastomere number of D3 embryos: ≤5, 6, 7, 8, 9, and ≥ 10 cells. The primary outcome was the live birth rate (LBR). The secondary outcomes were the clinical pregnancy rate, miscarriage rate, and neonatal outcomes. Statistical analyses were performed using a multivariate logistic regression model to explore the association between blastomere number in D3 embryos and LBR.Results The LBR significantly increased with the number of blastomeres in D3 embryos (28.4%, 36.4%, 42.5%, 46.1%, 45.2%, and 58.1%; p < 0.001). Furthermore, the results of the high- and low-quality blastocyst subgroup analyses showed significant differences in the LBR among the groups (p < 0.01). As the number of blastomeres in D3 embryos increased, the miscarriage rate significantly decreased (23.3%, 18.6%, 14.0%, 15.9%, 13.6%, and 8.9%; p < 0.05). However, the number of blastomeres did not affect perinatal outcomes. Multivariate logistic regression analysis after adjusting for confounding factors revealed significantly decreased LBR in the ≤ 5-cell group (adjusted odds ratio [aOR]: 0.627, 95% confidence interval [CI]: 0.442–0.891; p < 0.01) and significantly increased LBR in the ≥ 10-cell group (aOR: 1.612, 95% CI: 1.230–2.112; p < 0.01) compared with that in the 8-cell group.Conclusions The number of blastomeres in D3 embryos may be an important factor in selecting blastocysts during vitrified–thawed single blastocyst transfer cycles. The transfer of a single blastocyst arising from ≥ 10-blastomere D3 embryo may reduce the miscarriage rate and improve LBR.

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Analysis of Biochemical and Clinical Pregnancy Loss Between Frozen-Thawed Embryo Transfer of Blastocysts and Day 3 Cleavage Embryos in Young Women: A Comprehensive Comparison

Cited by 10 publications

References 46 publications

Analysis of Relevant Factors Affecting the Pregnancy Rate of Frozen-Thawed Embryo Transfer Cycle

Analysis of Relevant Factors Affecting the Pregnancy Rate of Frozen-Thawed Embryo Transfer Cycle

Association between duration of progesterone supplementation and clinical outcomes in artificial frozen-thawed embryo transfer cycles

Increased blastomere number in D3 embryos is associated with higher live birth rates in vitrified– thawed single blastocyst transfer cycles

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