Analysis of biodegradation performance of furfural and 5-hydroxymethylfurfural by Amorphotheca resinae ZN1

Ran, Hong; Zhang, Jian; Gao, Qinghai; Lin, Zhanglin; Bao, Jie

doi:10.1186/1754-6834-7-51

Cited by 109 publications

(84 citation statements)

References 44 publications

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“…Aerobic and anaerobic microorganisms ( Coniochaeta ligniaria, Ureibacillus thermosphaericus, Issatchenkia occidentalis, Enterobacter sp.) have been used for the removal of furfural and 5‐HMF from hydrolysates obtained from lignocellulosic biomass . These microorganisms use the metabolic pathway of Trudgill, which consists of various stages of oxidation and reduction catalyzed by enzymes such as aldehyde dehydrogenase, furoyl‐CoA synthetase or furoyl‐CoA dehydrogenase for the degradation of these compounds.…”

Section: Technologies For Inhibitor Removal From Hydrolysatesmentioning

confidence: 99%

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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The use of lignocellulosic biomass biofuels is an attractive alternative because they do not put food safety at risk, they are a renewable source, and their use is limited. The use of microorganisms has now become more widespread to take advantage of the carbohydrates present in this raw material (cellulose and hemicellulose) and the products of its hydrolysis (glucose, xylose, arabinose, galactose, and mannose). The fatty acids obtained from oleaginous microorganisms are potential sources for the production of drop‐in biofuels. Rhodosporidium sp. is an oleaginous yeast that accumulates up to 70% of its biomass in the form of lipids and is highly adaptable to different types of substrates. This review discusses the different factors and challenges (genetic modification of strains, pretreatments, and inhibitor effects) in obtaining lipid from lignocellulosic biomass using Rhodosporidium sp. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Technologies For Inhibitor Removal From Hydrolysatesmentioning

confidence: 99%

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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“…Recent research showed that overexpression of alcohol dehydrogenases (encoded by ADH ), transcription activator Msn2, oxidative stress regulator Yap1, and glucose-6-phosphate dehydrogenase, which were confirmed by transcriptional analysis, proteomic analysis, and disruption library screening, could improve furfural tolerance in yeast [19–22]. In S. cerevisiae , furfural could be converted into furfuryl alcohol by NADH-dependent alcohol dehydrogenase 1 [23–25]. Co-expression of transaldolase 1 and alcohol dehydrogenase 1 in recombinant xylose-fermenting S. cerevisiae improves the production of ethanol and xylitol in xylose medium in the presence of furfural [26].…”

Section: Introductionmentioning

confidence: 99%

Furfural tolerance and detoxification mechanism in Candida tropicalis

Wang

Cheng

Joshua

et al. 2016

Biotechnol Biofuels

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BackgroundCurrent biomass pretreatment by hydrothermal treatment (including acid hydrolysis, steam explosion, and high-temperature steaming) and ionic liquids generally generate inhibitors to the following fermentation process. Furfural is one of the typical inhibitors generated in hydrothermal treatment of biomass. Furfural could inhibit cell growth rate and decrease biofuel productivity of microbes. Candida tropicalis is a promising microbe for the production of biofuels and value-added chemicals using hemicellulose hydrolysate as carbon source. In this study, C. tropicalis showed a comparable ability of furfural tolerance during fermentation. We investigated the mechanism of C. tropicalis’s robust tolerance to furfural and relevant metabolic responses to obtain more information for metabolic engineering of microbes for efficient lignocellulose fermentation.Results Candida tropicalis showed comparable intrinsic tolerance to furfural and a fast rate of furfural detoxification. C. tropicalis’s half maximal inhibitory concentration for furfural with xylose as the sole carbon source was 3.69 g/L, which was higher than that of most wild-type microbes reported in the literature to our knowledge. Even though furfural prolonged the lag phase of C. tropicalis, the final biomass in the groups treated with 1 g/L furfural was slightly greater than that in the control groups. By real-time PCR analysis, we found that the expression of ADH1 in C. tropicalis (ctADH1) was induced by furfural and repressed by ethanol after furfural depletion. The expression of ctADH1 could be regulated by both furfural and ethanol. After the disruption of gene ctADH1, we found that C. tropicalis’s furfural tolerance was weakened. To further confirm the function of ctADH1 and enhance Escherichia coli’s furfural tolerance, ctADH1 was overexpressed in E. coli BL21 (DE3). The rate of furfural degradation in E. coli BL21 (DE3) with pET-ADH1 (high-copy plasmid) and pCS-ADH1 (medium-copy plasmid) was increased by 1.59-fold and 1.28-fold, respectively.Conclusions Candida tropicalis was a robust strain with intrinsic tolerance to inhibitor furfural. The mechanism of furfural detoxification and metabolic responses were identified by multiple analyses. Alcohol dehydrogenase 1 was confirmed to be responsible for furfural detoxification. C. tropicalis showed a complex regulation system during furfural detoxification to minimize adverse effects caused by furfural. Furthermore, the mechanism we uncovered in this work was successfully applied to enhance E. coli’s furfural tolerance by heterologous expression of ctADH1. The study provides deeper insights into strain modification for biofuel production by efficient lignocellulose fermentation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0668-x) contains supplementary material, which is available to authorized users.

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“…Furthermore, during most studies on biodegradation of furanic compounds, it was found that the reduction reaction of FAL and hydroxymethyfurfural (HMF) was often reversible. FOL can be oxidized to FAL again by alcohol dehydrogenase or aldehyde oxidase and then to FA by aldehyde oxidase from the cells . In this study, more FA produced at higher cell loading suggested that the decrease of FOL selectivity might be owing to the proceeding oxidization of produced FOL to FA.…”

Section: Resultsmentioning

confidence: 58%

“…In the case of glucose and maltose, the higher conversion was due to the much greater ability of S. cerevisiae to consume glucose and maltose than ethanol. However, ethanol is often an inhibitor for cell growth . To our knowledge, glucose is a broad‐spectrum carbon source and is less expensive than maltose.…”

Section: Resultsmentioning

confidence: 99%

“…Some high‐resistance strains, including Escherichia coli , Saccharomyces cerevisiae , and Pichia stipitis , have been selected and constructed . Furthermore, biochemical routes for the metabolism of FAL have been postulated; FAL is oxidized to furoic acid, which then enters the tricarboxylic acid cycle . But the biotransformation of FAL to highly valuable products has received little attention.…”

Section: Introductionmentioning

confidence: 99%

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Efficient whole‐cell biotransformation of furfural to furfuryl alcohol by Saccharomyces cerevisiae NL22

Yan

Xi-yue

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Furfuryl alcohol (FOL) is an upgraded product from biomass‐based furfural (FAL), which has wide application in fine chemical and polymer industries. FOL can be produced by chemical approaches, but harsh reaction conditions and subsequent safety problems often pose considerable challenges for chemical methods. In contrast to chemical methods that often do not meet the requirement for green chemistry, bio‐catalysis is an attractive alternative to replace chemical methods due to its mild reaction conditions and environmental friendliness. However, FAL is a well‐known inhibitor of many microorganisms. In this study, four different wild‐type cells were screened and employed for the bioreduction of FAL to FOL using a whole‐cell system. RESULTS Among these strains, Saccharomyces cerevisiae NL22 exhibited the best transformation ability from FAL into FOL. In order to improve the conversion of FAL and selectivity of FOL, optimization of whole‐cell biotransformation using S. cerevisiae NL22 was carried out. A maximal conversion of 98% and a high selectivity of 87.9% were obtained at 30 °C and 62 mmol L−1 FAL in the presence of 1.3 mol glucose/mol FAL within 8 h. A total of 122 mmol L−1 FOL was synthesized within 24 h reaction by a fed‐batch strategy. CONCLUSION The bioprocess developed in this study is a highly efficient method for FOL synthesis. The wild‐type strain S. cerevisiae NL22 might serve as a candidate for the production of FAL to FOL as an efficient biocatalyst. © 2019 Society of Chemical Industry

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Analysis of biodegradation performance of furfural and 5-hydroxymethylfurfural by Amorphotheca resinae ZN1

Cited by 109 publications

References 44 publications

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Furfural tolerance and detoxification mechanism in Candida tropicalis

Efficient whole‐cell biotransformation of furfural to furfuryl alcohol by Saccharomyces cerevisiae NL22

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