Challenges in lipid production from lignocellulosic biomass using <i>Rhodosporidium</i> sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González, Carlos S.; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Kermanshahi-pour, Azadeh; Avalos‐Ramírez, Antonio

doi:10.1002/bbb.1954

Cited by 43 publications

(16 citation statements)

References 95 publications

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“…However, the foremost challenge is the consumption of sugars in the presence of inhibitors that generally release into hydrolysate after the lignocellulose pretreatment. These compounds generally hinder the use of substrates by interfering in the metabolic pathways of microorganisms [5]. Hence, it is very important to use a robust strain that should be capable of consuming a wide variety of carbon sources as well as have high inhibitor tolerances.…”

Section: Introductionmentioning

confidence: 99%

“…toruloides, a basidiomycetes yeast, has been widely studied over the past years and reported to co-metabolize pentose and hexose sugars. R. toruloides was found to yield 100 g/L biomass and accumulate 50-70% lipid of its total DCW [5]. It is also known as red yeast because of the production of carotenoids, such as astaxanthin, β-carotene, and torularhodin.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

et al. 2020

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The study aims to explore microbial lipid production using an abundant and low-cost lignocellulosic biomass derived from forestry residues. Sugar-rich undetoxified hydrolysate was prepared using hardwood and softwood sawdust and used for lipid production as a carbon source from an oleaginous yeast, Rhodosporidium toruloides-1588. The maximum biomass obtained was 17.09 and 19.56 g/L in hardwood and softwood hydrolysate, respectively. Sugar consumption in both hydrolysates was >95%, with a maximum lipid accumulation of 36.68% at 104 h and 35.24% at 96 h. Moreover, R. toruloides-1588 exhibited tolerance to several toxic compounds such as phenols, organic acids and furans present in hydrolysates. The lipid characterization showed several monosaturated and polyunsaturated fatty acids, making it a potential feedstock for biofuels and oleochemicals production. This study confirms the credibility of R. toruloides-1588 as a suitable lipid producer using hydrolysates from forestry residues as a substrate. Additionally, lipids obtained from R. toruloides-1588 could be a potential feedstock for advanced biofuel production as well as for food and pharmaceutical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

et al. 2020

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“…Notably, to scale up the production of those intracellular products, it is essential to operate with high cell density cultures, which means that the processes are subjected to restriction due to inefficient oxygen supply. Also, lipid production has been well-known as an oxygen-demanding process [39], and oxygen availability has been identified as a major limiting factor for lipid production from lignocellulosic biomass [40]. Therefore, co-expression of the VHb gene with those functional genes may provide advanced strains.…”

Section: Discussionmentioning

confidence: 99%

Expression of VHb Improved Lipid Production in Rhodosporidium toruloides

et al. 2020

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The oleaginous yeast Rhodosporidium toruloides has emerged as a robust host for production of microbial lipids as alternative biofuel feedstocks. Oxygen supply is a limiting factor for microbial lipid production, as lipid biosynthesis is highly oxygen-demanding. Vitreoscilla hemoglobin (VHb) is a protein capable of promoting oxygen delivery for anabolism. In this study, we developed R. toruloides with VHb expression for improved lipid production. The VHb expression cassette was integrated into the R. toruloides chromosome via the Agrobacterium-mediated transformation. In shake flask cultures, the engineered strain 4#-13 produced 34% more lipids than the parental strain did. Results obtained under reduced aeration conditions in 3 L bioreactor showed that lipid titer and lipid yield of the engineered strain 4#-13 were 116% and 71%, respectively, higher than those of the parental strain. Under high cell density culture conditions, the engineered strain 4#-13 grew faster and produced 72% more lipids. Our results demonstrated that the VHb gene is functional in R. toruloides for promoting lipid production. The strains described here may be further engineered by integrating extra genetic parts to attain robust producers for more valuable products. This should improve the economics of microbial lipids to facilitate a sustainable production of biodiesel and other lipid-based biofuels.

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“…Apparently, R. toruloides has attracted major interests in academia and industry due to its unique physiological traits and vast imbedded potentials (Xu and Liu 2017 ; Park, Nicaud and Ledesma-Amaro 2018 ; Osorio-González et al . 2019a ; Saini et al . 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond

Wen

Zhang

Odoh

et al. 2020

FEMS Yeast Research

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ABSTRACT The red yeast Rhodosporidium toruloides naturally produces microbial lipids and carotenoids. In the past decade or so, many studies demonstrated R. toruloides as a promising platform for lipid production owing to its diverse substrate appetites, robust stress resistance and other favorable features. Also, significant progresses have been made in genome sequencing, multi-omic analysis and genome-scale modeling, thus illuminating the molecular basis behind its physiology, metabolism and response to environmental stresses. At the same time, genetic parts and tools are continuously being developed to manipulate this distinctive organism. Engineered R. toruloides strains are emerging for enhanced production of conventional lipids, functional lipids as well as other interesting metabolites. This review updates those progresses and highlights future directions for advanced biotechnological applications.

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Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Cited by 43 publications

References 95 publications

Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

Expression of VHb Improved Lipid Production in Rhodosporidium toruloides

Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond

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