Analysis of bovine sexed sperm for IVF from sorting to the embryo

Blondin, Patrick; Beaulieu, Michaël; Fournier, Valérie; Morin, Nathalie; Crawford, Lisa M.; Madan, Pavneesh; King, W. A.

doi:10.1016/j.theriogenology.2008.09.017

Cited by 86 publications

(89 citation statements)

References 28 publications

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“…Comparing the efficiency of embryo sexing versus sperm sexing method, recent publications report worse results with sexed sperm compared to unsexed sperm, when X chromosome-bearing fractions of sorted sperm were used for IVF (Blondin et al, 2009), for insemination (Schenk et al, 2009) and in multiple ovulation and embryo transfer (MOET) programs (Hayakawa et al, 2009;Peippo et al, 2009). The use of low-dose X-sorted spermatozoa for the insemination of superovulated embryo donors can improve the proportion of transferable female embryos produced but this potential may be not realised in commercial bovine practice, due to reduced fertilization rates using X-sorted spermatozoa (Peippo et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…However, recent results show that the capacitation of sorted freezethawed fractions of sperm and the developmental capacity of IVP embryos with desired sex is very low (Zhang et al, 2005;Katska-Ksiazkiewicz et al, 2006;Blondin et al, 2009). The pregnancy rates with low doses of sexed sperm drop about 10-40% compared to conventional doses of unsexed sperm (Andersson et al, 2006;Schenk et al, 2006Schenk et al, , 2009Seidel Jr., 2007;DeJarnette et al, 2009).…”

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confidence: 99%

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Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D 7 ) bovine embryos and to compare it with transfer of fresh female embryos. High quality embryos obtained from superovulated donors were biopsied and the embryonic cells (5-10) were analysed by PCR using specific primers for the Y-chromosome determinant. Fresh embryos were blade biopsied (n = 172) and subsequently transferred (ET) ipsilaterally to synchronized recipients. Selected embryos (n = 112) were biopsied by cell aspiration and cryopreserved for later transfer. Sex determination was successfully completed in 91.3% of fresh group embryos (44% female) and in 87.5% of freeze-thawed group embryos (45.9% female). The achieved pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. After transfer of freeze-thawed sexed (female) embryos, pregnancy was established in 48.8% of recipients. A similar conception rate was found in intact embryos (50.7%, control 2). The results clearly demonstrate that the microsurgical technique used and subsequent PCR sex analysis allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in embryo transfer programs.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…The use of sexed semen for in vivo (ET) (Baruseli et al, 2007;Hayakawa et al, 2009) or in vitro embryo production (IVP) (Lu et al, 1999;Wilson et al, 2006;Wheeler et al, 2006;Blondin et al, 2009;Xu et al, 2009) indicates a fundamental interaction between the variables and the produced results. Therefore, blastocyst production and/ or pregnancy rates must generate a substantial economic return.…”

Section: Introductionmentioning

confidence: 99%

“…Several papers have been written on both ET (Baruseli et al, 2007;Hayakawa et al, 2009) and IVP (Lu et al, 1999;Wilson et al, 2006;Wheeler et al, 2006;Blondin et al, 2009;Xu et al, 2009). These studies primarily describe the reproductive benefits of these technologies, which encompass strategies exhibiting positive correlation in synchronization methods, strategies for obtaining sexed semen and differentiated treatment of the donor that produces higher blastocyst numbers and/or pregnancy rates.…”

Section: Introductionmentioning

confidence: 99%

Simulation and economic analysis of bovine sex selection

et al. 2013

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-A simulation model implemented in the programming software Delphi XE ® was applied to evaluate sex selection in bovine. The hypothesis under investigation was that a dynamic model with stochastic and deterministic elements could detect the sexed semen technique to minimize pregnancy cost and to determine the adequate number of recipients required for in vivo (ET) and in vitro embryo production (IVP) in the proposed scenarios. Sex selection was compared through semen sexed using flow cytometry (C1) and density gradient centrifugation techniques (C2) in ET and IVP. Sensibility analyses were used to identify the adequate number of recipients for each scenario. This number was reinserted into the model to determine the biological and financial values that maximized ET and IVP using sexed semen (C1M and C2M). New scenarios showed that the density gradient technique minimized pregnancy cost based on the proposed scenarios. In addition, the adequate number of recipients (ET -C1M -115 and C2M -105)/(IVP -C1M -145 and C2M -140) per donor used was determined to minimize the pregnancy cost in all scenarios.

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“…Na produção de embriões os resultados são controversos encontrando menores taxas de clivagem (MORTON et al, 2007;BERMEJO-ALVAREZ, 2008) ou não encontrando diferenças entre o sêmen sexado e o sêmen convencional congelado com embriões produzidos in vitro (ZHANG et al, 2003;BLONDIN et al, 2009). Estes resultados expõem a idéia de que os espermatozóides sexados são afetados por algum procedimento dentro do processo da sexagem e/ou congelação.…”

Section: N T R O D U ç ã O | 24unclassified

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

Gallego¹

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A verdade é que, quando sua mente estava completamente alheia, ele tinha o pensamento mais estranho que qualquer lunático no mundo jamais tivera, pois lhe parecia razoável e necessário, tanto por uma questão de honra quanto para servir a nação, se tornar um cavaleiro errante e viajar pelo mundo em sua armadura e seu cavalo para buscar aventuras e envolver-se em tudo que ele havia lido sobre como os cavaleiros errantes se envolviam, aproveitando as oportunidades, colocando-se em perigo e corrigindo todo tipo de mal.-Miguel de Cervantes, Don Quixote. DEDICO.A Dios.A mis Papas Ramiro y Alba Lucia, su amor es la base de todas mis logros, Dios me bendijo al poder tenerlos a mi lado.A mi hermanita María del Pilar, eres la otra faz de una obra de arte, tu alegría y emotividad me llenan de felicidad. No te imaginas como te admiro.A mis abuelitas Ruth y Mary, Las abuelitas más dulces y tiernas que puedan existir, siempre las llevo en mi corazón.A mis Abuelos Libardo y Jorge (In memoriam), Sus vidas son una inspiración para mi. Es un honor llevar el apellido Mejia Gallego. Los Extraño A mi novia Dunia, Porque nuestros sueños se hagan realidad. LOS AMO !!!!! Agradecimientos.A mis tias Angela, Nohora y Liliana: Siempre han creido en mis capacidades, ello me ha llenado de confianza para llegar a donde estoy ahora, de todas he aprendido cosas importantes en la vida.Las llevo y las llevare en mi corazón siempre.A mis tios

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Analysis of bovine sexed sperm for IVF from sorting to the embryo

Cited by 86 publications

References 28 publications

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Simulation and economic analysis of bovine sex selection

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

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