Analysis of carbofuran, carbosulfan, isoprocarb, 3‐hydroxycarbofuran, and 3‐ketocarbofuran by micellar electrokinetic chromatography

Abstract

We developed an analytical method for the detection and quantitation of five pesticides and some of their metabolites - 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, carbosulfan, and isoprocarb - using micellar electrokinetic chromatography coupled with a UV-Vis detector. The optimum separation conditions were 20 mM phosphate buffer (pH 8.0) containing 15 mM sodium dodecyl sulfate. The detection wavelength was set at 200 nm and the applied voltage was 12.5 kV. Under these conditions, baseline separation of five pesticides was achieved in 15 min, and the detection limits (S/N = 3) of 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, carbosulfan, and isoprocarb were 0.3, 0.3, 0.3, 4.0, and 0.3 μM, respectively. The linear ranges for 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, and isoprocarb were between 1.0 and 50.0 μM and that for carbosulfan was between 10.0 and 100.0 μM, with R(2) larger than 0.995. When applied to the analysis of a carbofuran-spiked rice sample, this approach yielded results with excellent repeatability (3.3%, n = 5), reproducibility (4.5%, n = 5), separation efficiency (>2.1 × 10(4) theoretical plates), and recovery (95.5 ± 1.4%, n = 5).

Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography

“…All experiments were performed using an in‐house instrument, similar to that described previously , which consisted of a −20 kV high‐voltage power supply (ES20N‐20W/DAM, Gamma High Voltage Research Inc., Ormond Beach, FL, USA) and UV–Vis detector (SAPPHIRE 600 detector, ECOM, Prague, Czech Republic). Electropherograms were recorded and processed using DataApex Software (DataApex, Prague, Czech Republic).…”

Analysis of Seven Biogenic Amines and Two Amino Acids in Wines Using Micellar Electrokinetic Chromatography

“…To evaluate the effect of SDS concentration on the separation of analytes, the SDS concentration was varied from 15 to 30 mM in 5 mM phosphate buffer at pH 3.7. The CMC of SDS in water is ~8.2 mM [30]. The positive charged analytes interacted with negative charged SDS micelles to form ion pairs.…”

Metabolic Activation of Pesticide Isoprocarb Mediated by CYP3A4 and the Possible Correlation with Its Cytotoxicity

“…2,3 These pesticides are derived from carbamic acid with the introduction of different substituents. 4,5 Studies have shown that exposure to carbamate pesticides can cause endocrine disorders, cancer, deformities, and other serious diseases. 6,7 Isoprocarb (IPC) is one of the most important carbamate pesticides and works by contact and stomach poisoning of pests.…”