Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis

Matsuda, Motoo; Asami, Yasushi; Miyazawa, T.; Samata, Tetsuro; Isayama, Y; Honda, Masaaki; Ide, Yu

doi:10.1007/bf01839225

Cited by 12 publications

(6 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous ¢nding clearly demonstrated that pulsed-¢eld gel electrophoresis (PFGE) pro¢les after separate digestion with ApaI and NotI of the genomic DNAs from these three strains of T. equigenitalis, namely NCTC11184 T , Kentucky188 and EQ59, were distinctly di¡erent from each other (Matsuda et al, 1994(Matsuda et al, , 1999(Matsuda et al, , 2000Kagawa et al, 2001) and con¢rmed that heterogeneous genotypes of T. equigenitalis have a global distribution . In particular, the distinct genotypes between the strain NCTC11184 T and Kentucky188 were clearly demonstrated by the PFGE pro¢les as described previously (Matsuda et al, 1998(Matsuda et al, , 2000.…”

Section: Discussionmentioning

confidence: 93%

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

et al. 2006

Self Cite

View full text Add to dashboard Cite

The primer set for 16S rDNA amplified an amplicon of about 1500 bp in length for three strains of Taylorella equigenitalis (NCTC11184(T), Kentucky188 and EQ59). Sequence differences of the 16S rDNA among the six sequences, including three reference sequences, occurred at only a few nucleotide positions and thus, an extremely high sequence similarity of the 16S rDNA was first demonstrated among the six sequences. In addition, the primer set for 16S-23S rDNA internal spacer region (ISR) amplified two amplicons about 1300 bp and 1200 bp in length for the three strains. The ISRs were estimated to be about 920 bp in length for large ISR-A and about 830 bp for small ISR-B. Sequence alignment of the ISR-A and ISR-B demonstrated about 10 base differences between NCTC11184(T) and EQ59 and between Kentucky188 and EQ59. However, only minor sequence differences were demonstrated between the ISR-A and ISR-B from NCTC11184(T) and Kentucky188, respectively. A typical order of the intercistronic tRNAs with the 29 nucleotide spacer of 5'-16S rDNA-tRNA(Ile)-tRNA(Ala)-23S rDNA-3' was demonstrated in the all ISRs. The ISRs may be useful for the discrimination amongst isolates of T. equigenitalis if sequencing is employed.

show abstract

Section: Discussionmentioning

confidence: 93%

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…Preparation of agarose blocks from these cell suspensions and techniques for PFGE were performed as previously described (7,14,16). Genomic DNA was digested via restriction enzymes ApaI, NotI, and NaeI.…”

Section: Methodsmentioning

confidence: 99%

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Aalsburg¹,

Erdman²

2011

J Clin Microbiol

View full text Add to dashboard Cite

Taylorella equigenitalis is the etiologic agent of contagious equine metritis (CEM), a venereal disease of horses. A total of 82 strains of T. equigenitalis isolated in the United States were analyzed by pulsed-field gel electrophoresis (PFGE) after digestion of genomic DNA with restriction enzyme ApaI. Twenty-eight of those strains isolated from horses in the 2009 U.S. outbreak (CEM09) were further analyzed with NotI and NaeI enzymes. When ApaI alone was used for analysis, the 82 isolates clustered into 15 different genotypes that clearly defined groups of horses with known epidemiological connections. The PFGE profiles of the CEM09 isolates were indistinguishable after digestion with ApaI, NotI, and NaeI and did not match those of isolates from previous U.S. outbreaks in 1978 and 2006 or of any other isolate from the National Veterinary Services Laboratories (NVSL) culture library. Coupled with the fact that the CEM09 isolates are epidemiologically related, these results suggest a common source for the outbreak not linked to previous occurrences of CEM in the United States.

show abstract

“…The breeding of thoroughbreds in Japan and the absence of serological differences among tested strains of T. equigenitalis have also been described previously (MIYAZAWA et al 1995). The culture of strains, the preparation of agarose blocks and the procedure for PFGE after digestion with ApaI and with NotI, as well as the reasons for the choice of these restriction enzymes, have also been described in detail (MATSUDA et al 1994, MIYAZAWA et al 1995.…”

Section: Methodsmentioning

confidence: 99%

Detection of a common genotype among strains ofTaylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996

Matsuda

Miyazawa

Anzai

1999

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

We examined whether or not the genotype J could be detected among 21 new strains of T. equigenitalis isolated between 1994 and 1996 in Japan since our previous report (MiyazawaI et al. 1995). The respective pulsed‐field gel electrophoretic profiles of the 21 Japanese strains, as well as those of an old EQ59 used as a reference strain after separate digestion with the two restriction enzymes, ApaI and NotI, were essentially identical but differed from those of T. equigenitalis NCTC11184T and Kentucky 188, respectively. Hence, the 21 strains and EQ59 appeared to have a common genotype J. Consequently, no strains of T. equigenitalis with any genotype other than genotype J may have reached Japan from 1980 to 1996 and strains with the genotype J have survived in Japan since the first invasion of contagious equine metritis into Japan.

show abstract

Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis

Cited by 12 publications

References 7 publications

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Detection of a common genotype among strains ofTaylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996

Contact Info

Product

Resources

About