Analysis of Chromosomes 8 and 17 Aneuploidies in Laryngeal Squamous Cell Carcinoma by Fluorescence In Situ Hybridization

Öztürk, Kayhan; Acar, Hasan; Durmuş, Ercan; Öztürk, Adnan; Mutlu, Necip

doi:10.1097/00005537-200406000-00009

Cited by 3 publications

(3 citation statements)

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“…In our experiments, loss of P53 in most cases appeared to be related to deletion of chromosome 17, a frequent finding in squamous cell carcinoma 27 , rather than to a mutation. Others have observed this non-random deletion of chromosome 17 in cells aneuploid for other chromosomes 38 .…”

Section: Discussionsupporting

confidence: 49%

“…Even greater numbers of polyploid cells were observed following proliferation of these keratinocytes, suggesting that repeated cell division played a role in alteration of chromosome number. Keratinocytes exposed to allogeneic lymphocytes exhibited some of the chromosomal abnormalities frequently found in patients with squamous cell carcinoma 27 : aneuploidy for chromosomes 8, 7, 17 and 11, and tetraploidy. We observed loss of p53 in many of the anueploid cells-a factor which may be responsible for their continued growth and survival.…”

Section: Discussionmentioning

confidence: 99%

“…Tetraploidy and aneuploidy are present in most cases of SCC and complex chromosomal abnormalities correlate with poor prognosis 27–29 . Tetraploidy results from chromosome non-disjunction in late mitosis SCC 28, 30–34 and precedes the development of aneuploidy in some models 26 .…”

Section: Introductionmentioning

confidence: 99%

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Graft-versus-Host Disease: Role of Inflammation in the Development of Chromosomal Abnormalities of Keratinocytes

Sloand

Pfannes

Ling

et al. 2010

Biology of Blood and Marrow Transplantation

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Graft-versus-host disease (GVHD) is a major risk factor for secondary malignancy after hematopoietic stem cell transplantation. Squamous cell carcinoma (SCC) of the skin and mucous membranes are especially frequent in this setting where aneuploidy and tetraploidy are associated with aggressive disease. The current study is directed to the mechanism of neoplasia in this setting. Un-manipulated keratinocytes from areas of oral GVHD in 9 patients showed tetraploidy in 10–46% of cells when examined by florescence in situ hybridization (FISH). Keratinocytes isolated from biopsy sites of GVHD but not from normal tissue showed even greater numbers of tetraploid cells (mean 78%, range 15–85%; N=9) after culture. To mimic the inflammatory process in GVHD, allogeneic HLA- mismatched lymphocytes were mixed with normal keratinocytes. After two weeks, substantial numbers of aneuploid and tetraploid cells were evident in cultures with lymphocytes and with purified CD8 but not CD4 cells. Telomere length was substantially decreased in the lymphocyte-treated sample. No mutations were present in p53 gene, although haploinsufficiency for p53 due to loss of chromosome 17 was common in cells exposed to lymphocytes. These findings suggest that in GVHD, inflammation and repeated cell division correlates with the development of karyotypic abnormalities.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%