Analysis of composite nanofibrous layers by confocal Raman microscopy

Raman spectroscopy based method for the evaluation of compositional consistency of nanofibrous layers

“…An SVD analysis was used to prepare the calibration curve according to the procedure described in ref. 15. Spectra of all the solutions, prepared with increasing proportions of HA, were collected in a single data le including the spectra of pure HA and PEO.…”

“…The factor scores of the most distinct differences in the chemical composition are used for this purpose. 14,15 However, the values of these factor scores are mostly both negative and positive and moreover they are always in different value intervals, which may cause complications when evaluating the results and especially when comparing them. But if properly rescaled, they can represent the percentage proportion of a polymer in a spectrum.…”

“…13,14 The aim of this article is to show the possible applications of a combination of Raman spectroscopy and SVD for the checking of production of simple or composite nanobrous layers. The article follows our earlier research, 15 which outlined the possibility of using RS and SVD to monitor the distribution of polymers in a nanobrous layer but without the possibility of expressing component percentages. To allow this, results of measurements of a number of reference solutions were included in the SVD analysis, enabling the content proportions of the individual components within the measured area to be expressed in the form of a graph or a Raman map.…”

Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering

“…For PCL-BU ( Figures 2B,D ), a distinction could be made between the fingerprint regions resulting from the PCL chains and the urea blocks. When considering the PCL bands, peaks assigned to the C-C vibrations of the C-COO group (862 and 942 cm −1 ), skeletal stretching (1,067 and 1,098 cm −1 ), deformation vibrations of the CH 2 groups (1,303 and 1,442 cm −1 ) and vibrations caused by C=O vibration (1,728 cm −1 ) were identified ( 31 ). The fingerprint regions of the urea can be assigned to NCO and NCN vibrations (606 cm −1 ) and N-H stretching at 1,615 cm −1 ( 32 ).…”

Raman spectroscopy analysis of biodegradable electrospun nanofibers prepared from polymer blends

“…This type of analysis of produced nanofibrous materials could greatly aid in the optimization of the electrospinning process. The method was discussed in general in an earlier article [6,7], which included a presentation of the basic analytical possibilities of the combination of Raman spectroscopy and SVD, demonstrated on model samples of nanofibrous layers (PCL and PEO composites). There is also a publication focused on the specific application of this method and its calibration [8].…”