Raman spectroscopy analysis of biodegradable electrospun nanofibers prepared from polymer blends

Abstract

The electrospinning method was used for the preparation of polymeric nanofibrous layers made of polymers with possible use in the biomedical field. Various samples were prepared using combinations of polycaprolactone or poly(ethylene)oxide and hyaluronic acid. In both cases, nanofibrous layers were spun solely from a water solution. Using confocal Raman spectroscopy we were able to distinguish substantial variations in the distribution of the polymers caused by various preparation parameters and to characterize the prepared samples. Moreover, combining Raman spectroscopy with a mathematical method-singular value decomposition-enabled us to determine the ratios between the polymers used and thus to show the distribution of the present chemical constituents within a localized sample area. This may prove useful for the checking of production of nanofibrous products. Graphical abstract

Analysis of Chemisorbed Tribo-Film for Ceramic-on-Ceramic Hip Joint Prostheses by Raman Spectroscopy

“…There is a double peak near 1366 cm −1 and 1416 cm −1 , which is due to C–H bending [ 44 ] and due to C–N stretching and C–H deformation, respectively. Additionally, there is a peak at 1080 cm −1 due to C–OH bending and acetyl group [ 44 , 45 ]. The prominent peak at 992 cm −1 is due to ring breathing vibration [ 46 ] (p. 482).…”

Raman analysis of chemisorbed tribofilm for metal‐on‐polyethylene hip joint prostheses

“…The double peak near 1400 cm −1 is caused by C-N stretching and C-H deformation. The peak at 1090 cm −1 before testing and at 1079 cm −1 after the test is due to the C-OH bend in the acetyl group [38,40]. The sharp HA peak at 992 cm −1 is due to ring breathing [37], and the additional peak at 879 cm −1 is observable in other literature [38].…”

Siloxane-inorganic chemical crosslinking of hyaluronic acid – based hybrid hydrogels: Structural characterization

“…Fig.4B. The identified bands were assigned according to literature data(Alkrad, Mrestani, Stroehl, Wartewig, & Neubert, 2003;Cai, Neyer, Kuckuk, & Heise, 2010;Jayes, Hard, Séné, Parker, & Jayasooriya, 2003;Kotzianová, Rebícek, Pokorný, Hrbác, & Velebný, 2016;Qi et al, 2016;Sánchez-Téllez, Téllez-Jurado, Rodríguez-Lorenzo, Mazo et al, 2017; Sun, Yanagisawa, Kunimoto, Nakamura, & Homma, 2016;Sun, Yanagisawa, Kunimoto, Nakamura, & Homma, 2017;Tamayo & Rubio, 2010). Several Raman bands related to sodium hyaluronate hydrogels and silica-based hybrid materials are observed in the HA-based hybrid hydrogels Raman spectra.…”

“…Several Raman bands related to sodium hyaluronate hydrogels and silica-based hybrid materials are observed in the HA-based hybrid hydrogels Raman spectra. The most important Raman scattering due to different bond vibrations related to sodium hyaluronate (HA)(Alkrad et al, 2003;Kotzianová et al, 2016), chemical modification of HA with APTES(Sun et al, 2016(Sun et al, , 2017 and PDMS-modified SiO 2 crosslinked matrix(Qi et al, 2016; Sun et al, 2016) in the HA-based hybrid hydrogels are: 1) the band at 820 cm -1 is due to stretching vibrations of − − C O C bonds from the hyaluronic acid repetitive units (D-glucuronic acid and N-acetyl-D-glucosamine, linked via alternating β-(1→ 4) and β-(1→3) glycosidic bonds); 2) the band around 1415 cm -1 may be assigned to the bending vibration of CH 2 group bonded to the Si atom − Si CH ( ) 2 ; 3) the band at 1135 cm -1 is attributed to the − − Si O Si bond-bending vibration; 4) the bands around 1036 cm -1 and 945 cm -1 are assigned to − − O Si O bonds; 5) the band around 980 cm -1 is due to − Si OH bonds. Likewise, the following bands show the correct incorporation of PDMS into the SiO 2 network(Cai et al, 2010;Jayes et al, 2003;Sánchez-Téllez, Téllez-Jurado, Rodríguez-Lorenzo, Mazo et al, 2017;Tamayo & Rubio, 2010): 1) the bands around 1415 cm -1 and 1265 cm -1 are assigned to the asymmetric and symmetric bending vibration of CH 3 group from PDMS, respectively; 2) the band at 865 cm -1 is attributed to the symmetric rocking of CH 3 from PDMS; 3) the band at 790 cm -1 is due to − − C Si C asymmetric stretching vibration and to the rocking vibration of CH 3 group from PDMS; 4) the band at 707 cm -1 is due to − − C Si C symmetric stretching vibration; 5) the band at 689 cm -1 is attributed to − Si CH 3 symmetric rocking; 6) the band at 495 cm -1 is assigned to − − O Si O bonds.…”

“…4B. The identified bands were assigned according to literature data(Alkrad, Mrestani, Stroehl, Wartewig, & Neubert, 2003;Cai, Neyer, Kuckuk, & Heise, 2010;Jayes, Hard, Séné, Parker, & Jayasooriya, 2003;Kotzianová, Rebícek, Pokorný, Hrbác, & Velebný, 2016;Qi et al, 2016;…”