Analysis of differentially accumulated proteins associated with graft union formation in pecan ( Carya illinoensis )

Mo, Zhenghai; He, Haiyang; Su, Wenchuan; Peng, Fei

doi:10.1016/j.scienta.2017.06.005

Cited by 33 publications

(19 citation statements)

References 47 publications

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“…Expression of PM ATPase protein was differentially up‐regulated in the graft union 30 days after grafting of pecan ( Carya illinoensis ; Mo et al . ), together with up‐regulation of PM ATPase genes at 8, 15 and 30 day after grafting (Mo et al . ).…”

Section: Discussionmentioning

confidence: 99%

Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Zhou

Underhill

2018

Plant Biol J

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Breadfruit (Artocarpus altilis) is primarily grown as a staple tree crop for food security in Oceania. Significant wind damage has driven interest in developing its dwarfing rootstocks. Due to the predominantly vegetative propagation of the species, grafting onto interspecific seedlings is an approach to identifying dwarfing rootstocks. However, grafting of breadfruit onto unrelated Artocarpus species has not been investigated. Here we first report the success of breadfruit grafting onto interspecific rootstocks, marang (A. odoratissimus) and pedalai (A. sericicarpus). To address the low graft survival, we investigated the relationship of plasma membrane (PM) H -ATPase activity to graft success. We provide the first evidence for a positive correlation between PM H -ATPase activity and graft survival. The graft unions of successful grafts had higher PM H -ATPase activity compared to those of failed grafts. Rootstocks with low PM H -ATPase activity in leaf microsomes before grafting had lower graft survival than those with high enzyme activity, with graft success of 10% versus 60% and 0% versus 30% for marang and pedalai rootstocks, respectively. There was a positive correlation between graft success and the PM H -ATPase activity measured from the rootstock stem microsomes 2 months after grafting [marang, r(7) = 0.9203, P = 0.0004; pedalai (r(7) = 0. 8820, P = 0.0017]. Removal of scion's own roots decreased the leaf PM H -ATPase activity of grafted plants regardless of the final graft outcome. Recovery of the enzyme activity was only found in the successful grafts. The function of PM H -ATPase in graft union development and graft success improvement is discussed.

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Section: Discussionmentioning

confidence: 99%

Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Zhou

Underhill

2018

Plant Biol J

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“…The phloem at the base of the prepared cuttings mentioned previously was selected as the sample for the differential protein analysis. Protein extraction of the phloem was performed using a modified trichloroacetic acid-acetone precipitation method (Zhou et al, 2016), and the twodimensional gel electrophoresis procedure was referenced from differential protein analysis of pecan graft union formation (Mo et al, 2017). The two-dimensional gel electrophoresis procedure of the different cutting materials was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

“…The two-dimensional gel electrophoresis procedure of the different cutting materials was repeated three times. The gel-nitric acid was stained with silver and then scanned using an image scanner (BIO-RAD GS-800) (Mo et al, 2017). The scanned images were analyzed using PDQuest software to calculate the relative volume of different gels.…”

Section: Methodsmentioning

confidence: 99%

Differential Protein Analysis of Pecan Hardwood Cuttings

Fan¹,

Wang²,

Liu³

et al. 2019

horts

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Pecan cuttings are difficult for rooting. This study describes the pecan hardwood rooting process based on anatomic characteristics to understand root formation mechanisms of pecan cuttings. The expressed proteins of different periods during the adventitious rooting process of pecan seedling hardwood cuttings were identified and analyzed to evaluate the rooting mechanism. The expressed proteins of pecan cutting seedlings were also compared with other cultivar cuttings during the rooting period. Pecan seedling cuttings were developed at different air and substrate temperatures to induce root formation. Adventitious root formation of pecan hardwood cuttings was described, and the phloem at the base of the prepared cuttings was selected as the sample for the differential protein analysis. The results showed that adventitious root formation of pecan hardwood cuttings was the only product of callus differentiation, which originated from the cells of the cambium or vascular ray parenchyma. Such adventitious root primordia were developed from those calluses that formed the regenerative structure, and the expressed proteins during the adventitious rooting of pecan hardwood cutting were identified and analyzed by matrix-assisted laser desorption ionization–time of flight–mass spectrometry (MALDI-TOF-MS) to evaluate the rooting mechanism. Eight differentially expressed proteins were found in the rooting periods, and 15 differential proteins were found by comparing pecan cutting types, which were analyzed by peptide mass fingerprinting homology. The results show that the primordial cells were differentiated from the meristematic cells. Furthermore, the differentially expressed proteins contained energy metabolism proteins, adversity stress proteins, and signal transmission proteins. The energy metabolism-related proteins were adenosine triphosphate (ATP) synthase, photosynthesis-related proteins, and enolase. The adversity-stress proteins containing heat shock-related proteins and signal transmission proteins were mainly cytochrome enzymes and heme-binding proteins. Adventitious root formation of pecan cultivar hardwood cuttings was difficult. More trials should be performed from the potential aspects of high defensive protection and phloem morphologic structure.

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“…The reasons for reestablishing post-grafting are complex and involve many physiological and biochemical processes, such as the peroxidase. Peroxidase is intrinsically linked to the beginning of grafting process, because during the lignification there are specific functions in the lignin biosynthesis (Fernández-Pérez et al, 2015;Mo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of peroxidase may be used as a marker to predict the compatibility of pecan (Carya illinoensis) (Mo et al, 2017); likewise, are found similarly in peroxidase activity between scion and rootstock in interstock of the Prunus genus (Telles et al, 2009). Besides, studies claim that the presence of peroxidase isozymes in grafting is an experimental approach to predict the incompatibility reaction (Irisarri et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Can early peroxidase quantification detect graft-compatible in anonaceous rootstocks?

Báron¹,

Amaro²,

Macedo³

et al. 2018

Afr. J. Agric. Res.

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This study aimed to quantify the class III peroxidase activity in young plants of different species belonging to Annonaceae botany family, with high potential as a rootstock, for early detection of graftcompatible atemoya (Annona x atemoya Mabb.). The experimental design was randomized block, that evaluated the species araticum-de-terra-fria (Annona emarginata (Schltdl.) H. Rainer 'variety terra-fria'), araticum-mirim (Annona emarginata (Schltdl.) H. Rainer 'variety mirim) and biribá (Annona mucosa (Schltdl.) H. Rainer), with rootstock potential, and atemoya (scion), which were divided into four blocks, each with four plants. The peroxidase was quantified on the stem before grafting. Statistical analysis showed that only the biribá presented different peroxidase activity compared to atemoya plants; and araticum-mirim and araticum-de-terra-fria were similar to atemoya plants. Thus, the peroxidase activity could not be possible or be used as a tool in the early diagnosis of the graft-compatible in atemoya combinations.

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Analysis of differentially accumulated proteins associated with graft union formation in pecan ( Carya illinoensis )

Cited by 33 publications

References 47 publications

Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Differential Protein Analysis of Pecan Hardwood Cuttings

Can early peroxidase quantification detect graft-compatible in anonaceous rootstocks?

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Analysis of differentially accumulated proteins associated with graft union formation in pecan ( Carya illinoensis )

Cited by 33 publications

References 47 publications

Plasma membrane H+‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Plasma membrane H+‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Differential Protein Analysis of Pecan Hardwood Cuttings

Can early peroxidase quantification detect graft-compatible in anonaceous rootstocks?

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Product

Resources

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Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)

Plasma membrane H⁺‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)