Analysis of DNA polymorphism in a relict Uralian species, large-flowered foxglove (Digitalis grandiflora Mill.), using RAPD and ISSR markers

ABSTRACT. Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed. As verification of the CID validity, 8 randomly selected cultivars were investigated and proven to be well distinguished. In addition, 33 DNA bands were obtained, 20 (60.6%) of which were polymorphic. Genetic distances were calculated with a range of 0.032 to 1.402. Clustering analysis showed that the 64 tomato cultivars were divided into 4 groups with a similarity coefficient of 0.40. Using this novel strategy, with the same RAPD data, both CID and clustering analysis can simultaneously determine tomato cultivars and their genetic diversity.

Section: Discussionmentioning

confidence: 99%

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Cao

Zhou

et al. 2015

“…Even though several generations of DNA markers have been developed and used for cultivar identification (Saker et al, 2006;Chiu et al, 2010), genetic analysis (Boronnikova et al, 2007;Silvestrini et al, 2008;Bhau et al, 2009;Baysal et al, 2010), and in the process of thousands of papers published, they are not readily and efficiently used in genotyping. Whenever the need to distinguish some cultivars arises, we still do not know which primer or marker to use and how many primers or reactions are needed to identify some cultivars.…”

Section: Discussionmentioning

confidence: 99%

Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints

J¹,

Xc²,

Chang³

et al. 2011

ABSTRACT. Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plant individuals. These fingerprints can be used as efficient referential information for quick plant identification. We made a random amplified polymorphic DNA (RAPD) marker analysis of 68 pear cultivars. All pear genotypes could be distinguished by a combination of eight 11-mer primers. The efficiency of the method was further verified by correct identification of four cultivars randomly chosen from the initial 68. The advantages of this identification include use of fewer primers and ease of cultivar separation by the corresponding primers marked on the cultivar identification diagram. The cultivar identification diagram can efficiently serve for pear cultivar identification by readily providing the information Strategy for identification of Pyrus spp needed to separate cultivars. To the best of our knowledge, this is the most efficient strategy for identification of plant varieties using DNA markers; it could be employed for the development of the pear industry and for the utilization of DNA markers to identify other plant species.

“…The need to demystify science by developing strategies for applying new knowledge and technology to practical activities to solve the above problem is therefore both necessary and significant. DNA markers are a powerful technique that can be used to accurately identify plant cultivars and species, with several generations of DNA markers having been developed and used for cultivar identification (Saker et al, 2006;Chiu et al, 2010) and genetic analysis (Boronnikova et al, 2007;Silvestrini et al, 2008;Bhau et al, 2009;Baysal et al, 2010). Thousands of papers on this subject have also been published.…”

Section: Discussionmentioning

confidence: 99%

Methodology An improved strategy based on RAPD markers efficiently identified 95 peach cultivars

Wang²,

Ma³

et al. 2012

ABSTRACT. DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time-and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach genotypes (from the National Peach Germplasm Repository, in Nanjing, China) were distinguished by a combination of 54 primers. The utilization of the CID among these 95 peach cultivars was also verified by the identification of three randomly chosen groups of cultivars. This identification showed some advantages including the use of fewer primers and easy separation of all cultivars by the corresponding primers marked in the right position on the CID. This peach CID could provide the information to separate any peach cultivars of these 95, which may be of help to the peach industry in China and for the utilization of DNA markers to identify other plant species.