Methodology An improved strategy based on RAPD markers efficiently identified 95 peach cultivars

Abstract

ABSTRACT. DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time-and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach genotypes (from the National Peach Germplasm Repository, in Nanjing, China) were distinguished by a combination of 54 primers. The utilization of the CID among these 95 peach cultivars was also verified by the identification of three randomly chosen groups of cultivars. This identification showed some advantages including the use of fewer primers and easy separation of all cultivars by the corresponding primers marked in the right position on the CID. This peach CID could provide the information to separate any peach cultivars of these 95, which may be of help to the peach industry in China and for the utilization of DNA markers to identify other plant species.

Comparative analysis of DNA polymorphisms and phylogenetic relationships among Syzygium cumini Skeels based on phenotypic characters and RAPD technique

“…olive [26], mango [27], Peach [28], grape [29], guava [30] and Eucalyptus [31]. Few reports on the use of RAPD markers are also available to study the phylogenetic relationships among jamun accessions [32].…”

Molecular analysis of native cultivars of sweet cherry in Southern Italy

“…Among the narrow amount of molecular techniques already available in cherry, randomly amplified polymorphic DNA (RAPD) markers have proven to be a reliable marker system for genetic fingerprinting and also in determining the genetic relationships among germplasm collections. RAPD have the advantage of being simple, able to detect relatively small amounts of genetic variation and do not need prior information on the genome (Yu et al 2012). These technique has been already used successfully to reveal genetic variations both in cherry (Gerlach et al 1997;Hormaza 1999;Downey, Iezzoni 2000;Wuensch, Hormaza 2002) and other crops, as grapevine (Ulanovsky et al 2002;Kocsis et al 2005;Karataş, Ağaoğlu 2010), blueberry (Rowland, Levi 1994), peach (Downey, Iezzoni 2000), apricot (Di Vaio et al 2010) and Brassica oleracea (Dos Santos et al 1994).…”