Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves

Bruggmann, Rémy; Abderhalden, Olaf; Reymond, Philippe; Dudler, Robert

doi:10.1007/s11103-005-3099-9

Cited by 35 publications

(37 citation statements)

References 87 publications

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“…B. graminis f. sp. tritici infection of wheat caused increased expression of defenserelated transcripts in mesophyll cells bordering infected epidermal cells (Bruggmann et al 2005). Gjetting and associates (2007) studied gene expression in B. graminis f. sp.…”

Section: Time Course Analysis Identifies Biphasic Soybean Responses Tmentioning

confidence: 99%

Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

Mortel¹,

Recknor²,

Graham³

et al. 2007

MPMI

122

125

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Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. Within the first 12 h after inoculation (hai), which corresponds to fungal germination and penetration of the epidermal cells, differential gene expression changes were evident in both genotypes. mRNA expression of these genes mostly returned to levels found in mock-inoculated plants by 24 hai. In the susceptible genotype, gene expression remained unaffected by rust infection until 96 hai, a time period when rapid fungal growth began. In contrast, gene expression in the resistant genotype diverged from the mock-inoculated control earlier, at 72 h, demonstrating that Rpp2-mediated defenses were initiated prior to this time. These data suggest that ASR initially induces a non-specific response that is transient or is suppressed when early steps in colonization are completed in both soybean genotypes. The race-specific resistance phenotype of Rpp2 is manifested in massive gene expression changes after the initial response prior to the onset of rapid fungal growth that occurs in the susceptible genotype.

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Section: Time Course Analysis Identifies Biphasic Soybean Responses Tmentioning

confidence: 99%

Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

Mortel¹,

Recknor²,

Graham³

et al. 2007

MPMI

122

125

View full text Add to dashboard Cite

show abstract

“…Recently, high-throughput transcriptome analyses have helped define the responses triggered in plant tissues upon recognition of these fungal pathogens. These studies were performed under conditions promoting activation of defenses involving either host resistance (Caldo et al, 2004;Eckey et al, 2004;Eulgem et al, 2004;Zierold et al, 2005;Michel et al, 2006) or nonhost resistance (Zimmerli et al, 2004;Bruggmann et al, 2005). Under conditions leading to compatibility, basic gene expression changes have been examined in Vicia faba (Wirsel et al, 2001), barley (Hordeum vulgare; Gjetting et al, 2004), and Brassica oleracea (Casimiro et al, 2006).…”

mentioning

confidence: 99%

Genome-Wide Expression Profiling Arabidopsis at the Stage ofGolovinomyces cichoracearumHaustorium Formation

et al. 2008

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(T.S., K.D.)Compatibility between plants and obligate biotrophic fungi requires fungal mechanisms for efficiently obtaining nutrients and counteracting plant defenses under conditions that are expected to induce changes in the host transcriptome. A key step in the proliferation of biotrophic fungi is haustorium differentiation. Here we analyzed global gene expression patterns in Arabidopsis thaliana leaves during the formation of haustoria by Golovinomyces cichoracearum. At this time, the endogenous levels of salicylic acid (SA) and jasmonic acid (JA) were found to be enhanced. The responses of wild-type, npr1-1, and jar1-1 plants were used to categorize the sensitivity of gene expression changes to NPR1 and JAR1, which are components of the SA and JA signaling pathways, respectively. We found that the infection process was the major source of variation, with 70 genes identified as having similarly altered expression patterns regardless of plant genotype. In addition, principal component analysis (PCA) identified genes responding both to infection and to lack of functional JAR1 (17 genes) or NPR1 (18 genes), indicating that the JA and SA signaling pathways function as secondary sources of variation. Participation of these genes in the SA or JA pathways had not been described previously. We found that some of these genes may be sensitive to the balance between the SA and JA pathways, representing novel markers for the elucidation of cross-talk points between these signaling cascades. Conserved putative regulatory motifs were found in the promoter regions of each subset of genes. Collectively, our results indicate that gene expression changes in response to infection by obligate biotrophic fungi may support fungal nutrition by promoting alterations in host metabolism. In addition, these studies provide novel markers for the characterization of defense pathways and susceptibility features under this infection condition.

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“…Effects of syringolin A on the transcriptome of infected and uninfected wheat and Arabidopsis plants To monitor transcriptional changes in wheat, 307 cDNA clones representing 158 unigenes from powdery mildew infected, syringolin A sprayed wheat leaves were cloned by a suppression subtractive hybridization cloning procedure. These cDNAs were microarrayed onto glass slides together with 1088 cDNA-AFLP clones obtained from powdery mildewinfected wheat (Bruggmann et al, 2005). Microarray hybridization experiments were performed with probes derived from leaves, epidermal tissue, and mesophyll preparations of mildewed or uninfected wheat plants 12 h and 24 h after syringolin A or control treatment.…”

Section: Resultsmentioning

confidence: 99%

Syringolin A: Action on Plants, Regulation of Biosynthesis and Phylogenetic Occurrence of Structurally Related Compounds

Schellenberg

Ramel

Dudler

2008

Pseudomonas Syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics

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Cloning of the syringolin A synthetase genes has allowed us to build a detailed model of syringolin A synthesis based on the gene structure. This model in turn enabled us to clone the genes responsible for the synthesis of glidobactins (syn. cepafungins), antibiotics with a structure related to syringolin A that were reported to have antitumor activity, from an unknown species belonging to the order Burkholderiales. Comparisons to the approximately 700 complete eubacterial genomic sequences known resulted in the identification of a small but very intriguing group of pathogenic bacteria postulated to produce glidobacting-like molecules.

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Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves

Cited by 35 publications

References 87 publications

Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

Genome-Wide Expression Profiling Arabidopsis at the Stage ofGolovinomyces cichoracearumHaustorium Formation

Syringolin A: Action on Plants, Regulation of Biosynthesis and Phylogenetic Occurrence of Structurally Related Compounds

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