Analysis of Essential Leucine Residue for Catalytic Activity of Novel Thermostable Chitosanase by Site-Directed Mutagenesis

Yoon, Ho Geun; Yang, Sung Woo; Kim, Hee Yun; Kim, Hye Kyung; Shin, Dong Hoon; Hong, Bum Shik; Cho, Hong Yon

doi:10.1023/a:1007147214796

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…First, they are highly stable in most cases since dendrimers molecules are strongly adsorbed to the initial surface in contrast to the monopodal attachment of linear alkylbased self-assembled monolayers. Moreover, since such molecules contain a large number of peripheral reactive groups as well as a structure that is molecularly controllable (type of core, the choice of branch point, and distance between successive branch junctions), they turned out to be very efficient in influencing the self-assembly properties of biomolecules [11] such as antibodies or plasmids.…”

Section: Dendrimeric Patterned Surfacesmentioning

confidence: 99%

Nano-Structuring by Molecular Self-Assembly

Minelli¹,

Blondiaux²,

Losson³

et al. 2003

Chimia

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the new physical, chemical, or biological properties of surfaces structured using these methods. There is a special emphasis on structuring mechanisms relying on selfassembly, self-organization, and non-equilibrium processes, i.e. parallel approaches to the fabrication of large-area structures with high short-range order and some long-range order. In addition to this purely bottom-up approach based on self-organization of molecules, we also combine these selforganization methods with top-down, soft lithography techniques such as microfluidics for larger scale patterning of surfaces.Here we describe some different approaches to structuring surfaces. The first approach uses the ability of a spin-coated solution of two different homopolymers to demix and form micro-to nano-structured thin films with different morphologies. A very similar approach, used to create even smaller structures, with a higher degree of order is based on the phase separation of block-copolymers. This method gives chemically distinct domains with sizes ranging from 10 nm to 100 nm. Combining homopolymer and copolymer phase separation even allows the creation of polymeric films with structures on two length scales. The final example described here demonstrates the self-assembly and complexation properties of dendrimers, highly branched monodisperse macromolecules with welldefined structure, geometry, and monodispersity.Abstract: In contrast to conventional lithography techniques which will soon hit their limits in terms of feature size and fabrication cost, recent years have seen considerable progress in the development of selfassembling nano-structured surfaces. In the work reported here, surface structures on the submicron scale have been created from polymer, copolymer, nanoparticle, and dendrimer building blocks by employing phenomena such as self-assembly, self-organization and non-equilibrium processes. This low-cost approach is expected to result in a new generation of surfaces with novel physical and chemical properties.

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Section: Dendrimeric Patterned Surfacesmentioning

confidence: 99%

Nano-Structuring by Molecular Self-Assembly

Minelli¹,

Blondiaux²,

Losson³

et al. 2003

Chimia

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Microbial Chitinases: Natural Sources, Mutagenesis, and Directed Evolution to Obtain Thermophilic Counterparts

Sarma

Prakash

Das

et al. 2013

Thermophilic Microbes in Environmental and Industrial Biotechnology

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Recent Progress in Chitosanase Production of Monomer-Free Chitooligosaccharides: Bioprocess Strategies and Future Applications

Sinha

Chand

Tripathi

2016

Appl Biochem Biotechnol

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Biological activities of chitosan oligosaccharides (COS) are well documented, and numerous reports of COS production using specific and non-specific enzymes are available. However, strategies for improving the overall yield by making it monomer free need to be developed. Continuous enzymatic production from chitosan derived from marine wastes is desirable and is cost-effective. Isolation of potential microbes showing chitosanase activity from various ecological niches, gene cloning, enzyme immobilization, and fractionation/purification of COS are some areas, where lot of work is in progress. This review covers recent measures to improve monomer-free COS production using chitosanase/non-specific enzymes and purification/fractionation of these molecules using ultrafiltration and column chromatographic techniques. Various bioprocess strategies, gene cloning for enhanced chitosanase enzyme production, and other measures for COS yield improvements have also been covered in this review. COS derivative preparation as well as COS-coated nanoparticles for efficient drug delivery are being focused in recent studies.

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Analysis of Essential Leucine Residue for Catalytic Activity of Novel Thermostable Chitosanase by Site-Directed Mutagenesis

Cited by 5 publications

References 27 publications

Nano-Structuring by Molecular Self-Assembly

Nano-Structuring by Molecular Self-Assembly

Microbial Chitinases: Natural Sources, Mutagenesis, and Directed Evolution to Obtain Thermophilic Counterparts

Recent Progress in Chitosanase Production of Monomer-Free Chitooligosaccharides: Bioprocess Strategies and Future Applications

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