Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis

Tallini, Luciana R.; Pedrazza, Graziele Pereira Ramos; Bordignon, Sérgio Augusto de Loreto; Costa, Ana Carolina Oliveira; Steppe, Martin; Zuanazzi, José Ângelo Silveira

doi:10.1016/j.bjp.2015.04.003

Cited by 28 publications

(17 citation statements)

References 32 publications

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“…This physical–chemical characteristic of the solvent expresses its capability to absorb microwave radiation and convert it to thermal energy . Bearing this in mind, and based also on previous studies, different pure solvents, i.e. water, methanol and ethanol, and mixtures of these solvents in several ratios (1:4, 1:1, 4:1 v/v; organic solvent:water) were tested using a sample weight of 0.50 g and a solvent volume of 20 mL at 100 °C for 20 min.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have demonstrated that mulberries are polyphenol‐rich plants with a large variety of positive health effects such as hyperglycemic, antinephritis, and anti‐inflammatory properties . In fact, there is information about M. nigra fruits, but very little about their leaves . Thus, the need of new sources of antioxidants, as feasible, natural and inexpensive alternatives to synthetic antioxidants, associated with the recognized health benefits reported for mulberry plants, promote interest in the exploitation of mulberry leaves for the agro‐food and other related industries.…”

Section: Introductionmentioning

confidence: 99%

“…9 In fact, there is information about M. nigra fruits, 5,10-14 but very little about their leaves. 10,12,15,16 Thus, the need of new sources of antioxidants, as feasible, natural and inexpensive alternatives to synthetic antioxidants, associated with the recognized health benefits reported for mulberry plants, promote interest in the exploitation of mulberry leaves for the agro-food and other related industries. Extraction is usually the limiting analytical step in the yield of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

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Microwave‐assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition

Radojković

Moreira

Soares

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Morus nigra (mulberry) fruit has been reported as a source of bioactive compounds, although information about their leaves is very limited. Usually, they are considered wastes and have been traditionally used only for tea preparation. The main objective of this work was to explore the valorization of mulberry leaves by polyphenols microwave‐assisted extraction (MAE) and characterization of their antioxidant activity and phenolic composition. A 23 factorial design combined with response surface methodology were applied to characterize the effect of main microwave parameters on total phenolic content (TPC). RESULTS The optimized MAE conditions were 20 mL of ethanol:water (1:1; v/v), 120 °C, 28 min, 0.414 g and medium stirring speed. Under these conditions, TPC was 19.7 ± 2.0 mg gallic acid equivalents (GAE) g‐1 dry plant (DP), and antioxidant activity was 15.3 ± 1.0 mg ascorbic acid (AA) g‐1 DP (ferric reduction activity power –FRAP– assay), 18.6 ± 1.3 mg Trolox equivalents (TE) g‐1 DP (2,2‐diphenyl‐1‐picrylhydrazyl –DPPH– assay) and 186 ± 15 mg TE g‐1 DP (oxygen radical absorbance capacity –ORAC– assay). When compared with ultrasound extraction and Pharmacopeia reference method, MAE was more efficient, representing a valuable technology. Of the 13 compounds identified by HPLC, the most abundant were rutin, chlorogenic, β‐resorcylic and caffeic acids. CONCLUSION These results indicate that mulberry leaves are of potential interest for agro‐food industries as a new source of natural antioxidants. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microwave‐assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition

Radojković

Moreira

Soares

et al. 2018

J of Chemical Tech & Biotech

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“…During this study, a method was developed by using the HPLC technique in order to obtain a chromatographic system that was able to elute and provide good resolution in the separation of compounds from extracts (Mn-EtOH and Mn-AcOEt) obtained from leaves of M. nigra L. For this purpose, in a preliminary way, the chromatographic conditions of the method developed by Tallini et al (2015) were selected, in which modifications were made. Tallini et al (2015) performed the identification and quantification of kaempferol and quercetin in hydrolysed extracts of M. nigra using 0.01% TFA as the mobile phase A and 0.08% ACN as the mobile phase B. Moreover, Hunyadi et al, (2012) conducted a study to quantify chlorogenic acid, rutin, and isoquercetin in M. alba extracts using these same solvents as mobile phase.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of a high performance liquid chromatography-diode array detection (HPLC-DAD) method for the quantification of rutin and isoquercetin in Morus nigra L. (Moraceae)

Sampaio¹,

Souza²,

Sá³

et al. 2018

Afr. J. Biotechnol.

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Morus nigra L. is widely used by the population of the São Francisco Valley (Brazil) for the treatment of several diseases such as hypertension, diabetes, arthritis etc. The objective of this study was to develop and validate an analytical method that is capable of identifing and quantifing flavonoids in the ethanolic crude extract (Mn-EtOH) and its ethyl acetate phase (Mn-AcOEt) obtained from M. nigra leaves using high performance liquid chromatography (HPLC). The plant material was collected and processed to obtain Mn-EtOH, which was subjected to a process of liquid/liquid partition, from which the AcOEt phase was obtained. The method was developed using an reverse phase (RP) C-18 column (250 × 4.6 mm, 5 μm) as the stationary phase. A mixture of acidified water (0.01% trifluoroacetic acid): acetonitrile was used as the mobile phase in a gradient system with a flow rate of 0.8 mL/min at a wavelength of 340 nm for the detection and determination. The method developed was considered to be specific: linear (R 2 > 0.99), selective, accurate, and robust.

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“…Produz seus frutos com abundância na Ásia e está inteiramente aclimada e cultivada nas regiões sul e sudeste do Brasil. As plantas do gênero Morus têm importância e interesse de pesquisadores devido ao seu potencial fitoterápico e uso na medicina popular 10 .…”

Section: Características Botânicasunclassified

Morus nigra L.: revisão sistematizada das propriedades botânicas, fitoquímicas e farmacológicas

Oliveira¹,

Costa²,

Estevam³

et al. 2018

Arch Health Invest

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Morus nigra é uma espécie vegetal popularmente conhecida por Amora negra. Esta espécie amplamente cultivada na Ásia e nas regiões sul e sudeste do Brasil, possui importância econômica devido a sua ampla utilização na alimentação e confeitaria, sendo também utilizada na medicina popular como anti-inflamatório, diurético, antitussígeno, anti-hemorrágico, para o tratamento do climatério e de doenças hepáticas e renais. Diversos estudos têm sido conduzidos a respeito das atividades farmacológicas da Morus nigra. Neste trabalho de revisão narrativa da literatura foram explorados estudos nas bases de dados acerca da atividade hipolipemiante e de outras atividades farmacológicas dos compostos fenólicos, estilbenos, flavonoide, isoprenilados, cumarinas, cromonas e xantonas da Morus nigra, para verificar sua capacidade de diminuição dos níveis de colesterol total, triglicerídeos, LDL e ainda, aumento dos níveis de HDL, como também outras propriedades antioxidantes, anticarcinogênica, antimutagênica. Desse modo, conclui-se a necessidade da realização de mais estudos com Morus nigrae principalmente mais ensaios clínicos que avaliem a segurança e eficácia na utilização desta planta.Descritores: Farmacologia; Fitoterapia; Hiperlipidemias; Morus.

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Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis

Cited by 28 publications

References 32 publications

Microwave‐assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition

Microwave‐assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition

Development and validation of a high performance liquid chromatography-diode array detection (HPLC-DAD) method for the quantification of rutin and isoquercetin in Morus nigra L. (Moraceae)

Morus nigra L.: revisão sistematizada das propriedades botânicas, fitoquímicas e farmacológicas

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