Analysis of Floral Color Differences between Different Ecological Conditions of Clematis tangutica (Maxim.) Korsh

Guo, Xiaozhu; Gui, Weihua; Li, Juan; Li, Jiang; Sun, Xuemei

doi:10.3390/molecules28010462

Cited by 4 publications

(1 citation statement)

References 51 publications

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“…The biological replicate correlation was assessed using Pearson's correlation coefficient R . As the absolute value of R approaches 1, it indicates a higher degree of correlation between the two replicate samples 55 . Figure 6B illustrates a correlation ranging from 0.80 to 0.98 ( R > 0.80), indicating strong reproducibility within the group.…”

Section: Resultsmentioning

confidence: 94%

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Peng,

Li,

Zeng

et al. 2024

J of Cosmetic Dermatology

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BackgroundResearch has demonstrated the anti‐photoaging properties of glabridin and bakuchiol.MethodsThe impact of glabridin, glabridin + bakuchiol, and bakuchiol on the levels of tumor necrosis factor alpha (TNF‐α) and interleukin‐1 beta (IL‐1β) in mice skin fibroblasts was observed. Furthermore, we investigated the potential roles of fibronectin (FN), interferon‐γ (IFN‐γ), interleukin‐22 (IL‐22), and transforming growth factor‐β (TGF‐β) in the tissues, and evaluated their impact on the enzymatic levels in the skin. In conjunction with transcriptomic analysis, metabolomic profiling, and network pharmacology, all samples underwent comprehensive metabolomic and principal component analysis. The Venny2.1 method was utilized to identify variances in shared metabolites between the treatment group and the UVB group, as well as between the UVB group and the control group. Subsequently, a cluster heat map was generated to forecast and analyze metabolic pathways and targets.ResultsThe outcomes from the hematoxylin and eosin and toluidine blue staining revealed that glabridin and bakuchiol markedly decreased dermal thickness and suppressed mast cell infiltration in photoaged mice. Immunohistochemistry and Elisa analysis revealed that glabridin and bakuchiol effectively attenuated the levels of pro‐inflammatory factors, including IL‐1β, tumor necrosis factor‐α, IL‐22, and IFN‐γ. Furthermore, an increase in the levels of anti‐inflammatory factors such as FN and TGF‐β was also observed. The determination of the contents of superoxide dismutase, hydroxypropyltransferase and malondialdehyde in mice dorsal skin revealed that glabridin and bakuchiol not only elevated the levels of superoxide dismutase and hydroxyproline, but also reduced malondialdehyde content. Due to the limited number of shared differential metabolites exclusively within Kyoto Encyclopedia of Genes and Genomes, comprehensive pathway enrichment analysis was not feasible.ConclusionThis study demonstrates that glabridin and bakuchiol effectively impede photoaging and alleviate skin inflammation in mice.

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Section: Resultsmentioning

confidence: 94%

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Peng,

Li,

Zeng

et al. 2024

J of Cosmetic Dermatology

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The complete chloroplast genome of the C. tangutica (Maxim.) Korsh. and an adaptive evolutionary analysis of the ycf2 gene

Guo,

Jiang,

Yang

et al. 2024

Preprint

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Clematis. tangutica (Maxim.) Korsh. is widely cultivated in Northwest China. The whole plant was called “Ye-Mang-Na-Bao” in traditional Tibetan medicine. In this study, Illumina sequencing technology was utilized to assemble and annotate the complete chloroplast genome sequences of it. The total length was 159, 584 bp, including four conserved regions: A pair of reverse repeat regions (IRa 31,042 bp and IRb 31,042 bp), a large single-copy region (79,515 bp), and a small single-copy region (17,985 bp). The genome had a total of 136 genes, with 16 presented in the reverse direction in the IR region. A total of 23 simple sequence repeats (SSRs) were identified in the coding and non-coding regions, most of which were biased toward A/T bases. A total of 15 SSRs were distributed in the non-coding regions. A comparative analysis of the chloroplast genome sequence of the C. tangutica (Maxim.) Krosh. and other species of the Ranunculaceae revealed that the chloroplast genome sequences of plants of the Ranunculaceae were highly conserved. Differences were observed in 13 gene loci in the coding region, with the degree of differentiation of the ycf2 gene being the most obvious. A phylogenetic analysis showed that Clematis glauca had the closest relationship with C. tangutica (Maxim.) Krosh., both members of the Clematis genus. Selective locus detection of the ycf2 gene in nine species of the Ranunculaceae was performed to explore adaptive evolution traits of the ycf2 gene in it. The results show that there are significant and extremely significant positive selection sites at the 103L, 352L, 356S, 362S, 363D, 366P, 368C, 369L, 374R, 376F, 377T and 382Q loci, respectively, indicating that the ycf2 gene has been subject to adaptive evolution.Insights from our assessment of the complete chloroplast genome sequences of C. tangutica (Maxim.) Krosh. will aid in the in-depth study of the evolutionary relationship of the Ranunculaceae and provide significant sequencing information for the genetic improvement of it.

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Integrated metabolomic and transcriptomic strategies to reveal adaptive mechanisms in barley plant during germination stage under waterlogging stress

luan,

gao,

et al. 2024

Preprint

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Waterlogging stress is one of prominent abiotic stresses that has a significant impact on the yield and quality of barley. Seed germination is crucial for seedling establishment and is highly sensitive to waterlogging stress. However, there is a limited understanding of the regulatory mechanisms of gene expression and metabolic processes in barley during the germination stage under waterlogging stress. This study aimed to investigate the metabolome and transcriptome responses in germinating barley seeds under waterlogging stress. The findings of the study revealed that waterlogging stress sharply decreased seedling growth. The tolerant genotype (LLZDM) exhibited higher levels of antioxidase activities and lower malondialdehyde (MDA) content in comparison to the sensitive genotype (NN). In addition, waterlogging induced 86 and 85 differentially expressed metabolites (DEMs) in LLZDM and NN, respectively. Concurrently, transcriptome analysis identified 1776 and 839 differentially expressed genes (DEGs) in LLZDM and NN, respectively. Notably, the expression of genes associated with redox reactions, hormone regulation, and other biological processes were altered in response to waterlogging stress. Furthermore, the integrated transcriptomic and metabolomic analyses revealed that the DEGs and DEMs implicated in mitigating waterlogging stress primarily pertained to the regulation of pyruvate metabolism and flavonoid biosynthesis. Moreover, waterlogging might promote flavonoid biosynthesis by regulating 15 flavonoid-related genes and 10 metabolites. The present research provides deeper insights into overall understanding waterlogging-tolerant mechanisms in barley during the germination process.

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Analysis of Floral Color Differences between Different Ecological Conditions of Clematis tangutica (Maxim.) Korsh

Cited by 4 publications

References 51 publications

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

The complete chloroplast genome of the C. tangutica (Maxim.) Korsh. and an adaptive evolutionary analysis of the ycf2 gene

Integrated metabolomic and transcriptomic strategies to reveal adaptive mechanisms in barley plant during germination stage under waterlogging stress

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