Analysis of Fructus Arctii from Different Regions of China by HPLC Coupled with Chemometrics Methods

Abstract

Summary.To evaluate the quality of Fructus Arctii, an accurate and reliable method of high-performance liquid chromatography/diode array detection-electrospray ionization-mass spectrometry (HPLC/DAD-ESI-MS) was developed. Nine compounds, including chlorogenic acid, caffeic acid, trans-p-hydroxycinnamic acid, arctiin, arctignan A, ethyl caffeate, matairesinol, arctigenin, and lappaol B, were determined simultaneously in 19 batches of Fructus Arctii samples collected from different localities. Nineteen common peaks were identified or tentatively assigned by comparing their mass spectrometric data with reference compounds, self-established compound library, and published literatures. Also, the 19 common peaks were selected as characteristic peaks to assess the similarity of chromatographic fingerprinting of these samples. Moreover, hierarchical clustering analysis (HCA) and principal components analysis (PCA) were successfully applied to demonstrate the variability of samples. The results indicated the content of nine compounds that varied greatly among the samples, and 19 samples collected from different localities could be discriminated. Furthermore, chlorogenic acid, arctiin, and arctigenin were found to be chemical markers for evaluating the quality of Fructus Arctii.

Purification of lignans from Fructus Arctii using off‐line two‐dimensional supercritical fluid chromatography/reversed‐phase liquid chromatography

“…These pure compounds were then characterized by MS and NMR spectra. Based on comparison of the spectral data with the literature values , the results showed that they were all lignans with similar structures. The data of structure, purity and weight of compounds was listed in Table and the NMR and MS data are illustrated in the Supporting Information.…”

Quality control of camellia oil based on HPLC fingerprinting techniques combined with chemometric methods for triglycerides

“…It was reported in the literature that the RSD of the peak area of the characteristic peak was less than 3%, which meant that the method met requirements of fingerprint analysis. [ 40 ] Therefore, the established chromatography method could be used for the quality evaluation of camellia oil.…”

Quality evaluation of moluodan concentrated pill using high‐performance liquid chromatography fingerprinting coupled with chemometrics

“…After reducing dimensions, every sample obtained a score plot and based on the score plot, a classification was made . HCA is an intuitive classification method, dividing samples into categories according to the similar distance degree (in this study, the single linkage method was used) . These two methods could support each other to some degree.…”