Quality evaluation of moluodan concentrated pill using high‐performance liquid chromatography fingerprinting coupled with chemometrics

Tao, Lingyan; Zhang, Qing; Wu, Yongjiang; Liu, Xuesong

doi:10.1002/jssc.201600895

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…As for compounds which had low content, especially Ginseng Radix et Rhizoma, Rehmanniae Radix, and Poria, or were difficult to be completely separated, we used LC-MS/MS to quantify them. The candidate ingredients were chosen based on the references of pertinent herbs, including the following 14 chemical components: XS and XZ from the minister medicine of Xanthii fructus [ 21 ]; JA and JB from the minister medicine of Ziziphi Spinosae Semen [ 22 ]; MPA from the minister medicine of Ophiopogonis Radix [ 23 ]; RD from the minister medicine of Rehmanniae Radix [ 24 ]; GRF, GRG, GRR, and GRB from the minister medicine of Ginseng Radix et Rhizoma [ 25 ]; DA, PAC, and ATA from the adjuvants medicine of Poria [ 26 ]; and PX from adjuvants medicine of the guide medicine of Polygalae Radix [ 27 ]. The chemical structures of the 25 compounds are shown in Figure S5 .…”

Section: Resultsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.

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Section: Resultsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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“…The mobile phase was composed of 70% methanol (A) and 30% water (B) with a 0.8 mL/min eluent velocity, under a detection temperature of 30 °C. The UV detection wavelength of GA was set at 254 nm. − The UV detection wavelength of Res was set at 306 nm. − The content of GA was calculated according to the standard curve ( y = 18666 x – 67.804, R adj 2 = 0.9999). The content of Res was calculated according to the standard curve ( y = 71002 x + 65.388, R adj 2 = 0.9999).…”

Section: Methodsmentioning

confidence: 99%

Resveratrol Triggered the Quick Self-Assembly of Gallic Acid into Therapeutic Hydrogels for Healing of Bacterially Infected Wounds

et al. 2022

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Programing self-assembly of naturally bioactive molecules has been a wide topic of great significance for biomedical uses. Despite the fact that plant-derived polyphenols with catechol or pyrogallol moieties have been widely studied to construct nanocomplexes or nanocoatings via self-polymerization, there is no report on the self-assembly of these polyphenols into therapeutic hydrogels for potential applications. Here, we reported that adding a very small amount of resveratrol (Res) into the gallic acid (GA) aqueous solution could trigger the quick self-assembly of GA to form a fibrous hydrogel within 5 min through hydrogen bonds and π−π interactions. The length of GA/Res (GR) fibrils in gels varied from 100 to 1000 microns, with a diameter of around 1 μm. Notably, these GR hydrogels showed excellent colloid stability, providing better slow release and outstanding biocompatibility. Also, in vivo experiments indicated the hydrogels had high antibacterial effects and excellent wound healing capabilities in a total skin defect model via regulating the expression of inflammatory factors (IL-6, IL-1β, and TNF-α) due to the release of therapeutic agents (GA and Res) into the matrix. Overall, our results provide a new strategy to accelerate self-assembly of GA by adding Res to form hydrogels, which is further proved as a promising therapeutic carrier for wound healing.

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“…The developing solvent systems, such as ethyl acetate/formic acid/acetic acid/water, ethyl acetate/nbutanol/ acetic acid/water, butyl acetate/formic acid/acetic (12), isoquercitrin (13), wogonin (15), and genkwanin (16) acid/water with different ratios were investigated on the RP system. Results showed that the appropriate R f values and entire separation of four compounds obtained by developing solvent system of ethyl acetate/formic acid/acetic acid/water (10:1.5:1.5:1.0, v/v/v/v).…”

Section: Optimization Of Hptlc Analysismentioning

confidence: 99%

“…Up to now, a few methods have been reported to evaluate the quality of TCM [15][16][17][18][19][20][21]. For Herba Leonuri, the authentication and quantification of stachydrine and leonurine using normal-phase (NP) and reversed-phase (RP) chromatographic methods has been adopt in Chinese Pharmacopoeia (2015 edition) [22].…”

mentioning

confidence: 99%

Quality assessment of Herba Leonuri based on the analysis of multiple components using normal‐ and reversed‐phase chromatographic methods

Dong

Hou

et al. 2017

J of Separation Science

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A novel, improved, and comprehensive method for quality evaluation and discrimination of Herba Leonuri has been developed and validated based on normal- and reversed-phase chromatographic methods. To identify Herba Leonuri, normal- and reversed-phase high-performance thin-layer chromatography fingerprints were obtained by comparing the colors and R values of the bands, and reversed-phase high-performance liquid chromatography fingerprints were obtained by using an Agilent Poroshell 120 SB-C18 within 28 min. By similarity analysis and hierarchical clustering analysis, we show that there are similar chromatographic patterns in Herba Leonuri samples, but significant differences in counterfeits and variants. To quantify the bio-active components of Herba Leonuri, reversed-phase high-performance liquid chromatography was performed to analyze syringate, leonurine, quercetin-3-O-robiniaglycoside, hyperoside, rutin, isoquercitrin, wogonin, and genkwanin simultaneously by single standard to determine multi-components method with rutin as internal standard. Meanwhile, normal-phase high-performance liquid chromatography was performed by using an Agilent ZORBAX HILIC Plus within 6 min to determine trigonelline and stachydrine using trigonelline as internal standard. Innovatively, among these compounds, bio-active components of quercetin-3-O-robiniaglycoside and trigonelline were first determined in Herba Leonuri. In general, the method integrating multi-chromatographic analyses offered an efficient way for the standardization and identification of Herba Leonuri.

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Quality evaluation of moluodan concentrated pill using high‐performance liquid chromatography fingerprinting coupled with chemometrics

Cited by 11 publications

References 24 publications

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Resveratrol Triggered the Quick Self-Assembly of Gallic Acid into Therapeutic Hydrogels for Healing of Bacterially Infected Wounds

Quality assessment of Herba Leonuri based on the analysis of multiple components using normal‐ and reversed‐phase chromatographic methods

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